DOI: 10.1111/j.1471-4159.1985.tb05504.x. Dephosphorylation of Microtubule‐Associated Protein 2, τ Factor, and Tubulin by Calcineurin

Dephosphorylation of Microtubule‐Associated Protein 2, τ Factor, and Tubulin by Calcineurin

10.1111/j.1471-4159.1985.tb05504.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Calcineurin dephosphorylated microtubule‐associated protein 2 (MAP2) and τ factor phosphorylated by cyclic AMP‐dependent and Ca2+, calmodulin‐dependent protein kinases from the brain. Tubulin, only phosphorylated by the Ca2+, calmodulin‐dependent protein kinase, served as substrate for calcineurin. The concentrations of calmodulin required to give half‐maximal activation of calcineurin were 21 and 16 nM with MAP2 and τ factor as substrates, respectively. The Km and Vmaxvalues were in ranges of 1–3 μM and 0.4–1.7 μmol/mg/ min, respectively, for MAP2 and τ factor. The Km value for tubulin was in a similar range, but the Vmax value was lower. The peptide map analysis revealed that calcineurin dephosphorylated MAP2 and τ factor universally, but not in a site‐specific manner. The autophosphorylated Ca2+, calmodulin‐dependent protein kinase was not dephosphorylated by calcineurin. These results suggest that calcineurin plays an important role in the functions of microtubules via dephosphorylation.

Bibliography

Goto, S., Yamamoto, H., Fukunaga, K., Iwasa, T., Matsukado, Y., & Miyamoto, E. (1985). Dephosphorylation of MicrotubuleâAssociated Protein 2, Ï Factor, and Tubulin by Calcineurin. Journal of Neurochemistry, 45(1), 276â283. Portico.

Authors 6

Satoshi Goto (first)
Hideyuki Yamamoto (additional)
Kohji Fukunaga (additional)
Takafumi Iwasa (additional)
Yasuhiko Matsukado (additional)
Eishichi Miyamoto (additional)

References 54 Referenced 145

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{'key': 'e_1_2_2_55_1', 'first-page': '1313', 'article-title': 'Calmodulins from muscles of marine invertebrates, scallop and sea anemone', 'volume': '87', 'author': 'Yazawa M.', 'year': '1980', 'journal-title': 'Comparison with calmodulins from rabbit skeletal muscle and pig brain. J. Biochem.'} / Comparison with calmodulins from rabbit skeletal muscle and pig brain. J. Biochem. / Calmodulins from muscles of marine invertebrates, scallop and sea anemone by Yazawa M. (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 1:06 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 12:33 p.m.)
Indexed	1 week, 5 days ago (Aug. 19, 2025, 7:11 a.m.)
Issued	40 years, 1 month ago (July 1, 1985)
Published	40 years, 1 month ago (July 1, 1985)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	40 years, 1 month ago (July 1, 1985)

Funders 0

None

BibTeX

@article{Goto_1985, title={Dephosphorylation of Microtubule‐Associated Protein 2, τ Factor, and Tubulin by Calcineurin}, volume={45}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1985.tb05504.x}, DOI={10.1111/j.1471-4159.1985.tb05504.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Goto, Satoshi and Yamamoto, Hideyuki and Fukunaga, Kohji and Iwasa, Takafumi and Matsukado, Yasuhiko and Miyamoto, Eishichi}, year={1985}, month=jul, pages={276–283} }

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