DOI: 10.1111/j.1471-4159.1984.tb02744.x. Cell‐Free Synthesis of Myelin Basic Proteins in Normal and Dysmyelinating Mutant Mice

Cell‐Free Synthesis of Myelin Basic Proteins in Normal and Dysmyelinating Mutant Mice

10.1111/j.1471-4159.1984.tb02744.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Total polyribosomes were isolated from the brains of 16–20‐day C57BL/6 mice, four neurological mutants (qk/qk, shi/shi, mld/mld, and jp/Y), and four heterozygote or littermate controls (qk/+, shi/+, mld, and jp littermates) and translated in a homologous, cell‐free system. No differences were observed among the nine genotypes in either the yield of polysomes (32.2 ± 0.6 A260/g brain) or in the incorporation of [35S]methionine into trichloroacetic acid‐precipitable protein. However, when the four myelin basic proteins (BPs) were isolated from the translation mixtures little incorporation of [35S]methionine into the BPs was noted in those assays directed by polysomes from mld/mld or from shi/shi animals. Compared with C57BL/6 polysomes, mld littermate and shi/+ polysomes incorporated approximately half the levels of label into the four BPs while qk/+ and qk/qk incorporated normal and close‐to‐normal levels. Polysomes from jp littermates and jp/Y brains synthesized 66% and < 15% of the levels of the 14K BP compared with C57BL/6 polysomes. Incorporation of label into the other three BPs was normal with jp littermate polysomes and about half the control levels with jp/Y polysomes. The data indicate that shi/shi and mld/mld mutants either produce altered BPs not recognized by our antibody or synthesize very low levels of BP. The data provide additional support for the notion that the qk/qk mutant synthesizes much higher levels of MBP than are incorporated into myelin. They also indicate that in the jimpy mutant the synthesis of the four BPs is affected to differing extents; thus, the mutant cannot be easily characterized as either an “assembly” or “synthesis” defect.

Bibliography

Campagnoni, A. T., Campagnoni, C. W., Bourre, J. âM., Jacque, C., & Baumann, N. (1984). CellâFree Synthesis of Myelin Basic Proteins in Normal and Dysmyelinating Mutant Mice. Journal of Neurochemistry, 42(3), 733â739. Portico.

Authors 5

A. T. Campagnoni (first)
C. W. Campagnoni (additional)
J.‐M. Bourre (additional)
C. Jacque (additional)
N. Baumann (additional)

References 45 Referenced 44

10.1016/S0021-9258(18)43260-7 / J. Biol. Chem. / In vivo phosphorylation of two myelin basic proteins of developing rabbit brain by Agrawal H. C. (1981)
10.1042/bj2010039 / Biochem. J. / Phosphorylation in vivo of four basic proteins of rat brain myelin by Agrawal H. C. (1982)
{'key': 'e_1_2_2_4_1', 'first-page': '103', 'article-title': 'Level and polymorphism of myelin basic protein in shiverer mice', 'volume': '12', 'author': 'Barbarese E.', 'year': '1981', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Level and polymorphism of myelin basic protein in shiverer mice by Barbarese E. (1981)
10.1073/pnas.74.8.3360
10.1111/j.1471-4159.1979.tb11082.x
{'key': 'e_1_2_2_7_1', 'first-page': '565', 'volume-title': 'Neurological Mutations Affecting Myelination', 'author': 'Baumann N.', 'year': '1980'} / Neurological Mutations Affecting Myelination by Baumann N. (1980)
10.1111/j.1471-4159.1978.tb12479.x
10.1007/BF00965788
{'key': 'e_1_2_2_10_1', 'first-page': '413', 'volume-title': 'Neurological Mutations Affecting Myelinalion, INSERM Symposium No. 14', 'author': 'Braun P. E.', 'year': '1980'} / Neurological Mutations Affecting Myelinalion, INSERM Symposium No. 14 by Braun P. E. (1980)
10.1016/0006-8993(77)90404-8
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10.1111/j.1471-4159.1977.tb10430.x
{'key': 'e_1_2_2_14_1', 'first-page': '256', 'article-title': 'Myelin basic protein gene expression in normal, quaking, jimpy and msd mice', 'volume': '13', 'author': 'Carnow T. B.', 'year': '1982', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Myelin basic protein gene expression in normal, quaking, jimpy and msd mice by Carnow T. B. (1982)
{'key': 'e_1_2_2_15_1', 'first-page': '207', 'article-title': 'Synthesis and degradation of myelin basic protein in normal and jimpy mouse brain', 'volume': '6', 'author': 'Carson J. H.', 'year': '1975', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Synthesis and degradation of myelin basic protein in normal and jimpy mouse brain by Carson J. H. (1975)
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10.1002/jnr.490060305
10.1016/0003-2697(79)90165-9
10.1093/oxfordjournals.jhered.a108850 / J. Heredity / Myelin deficient, a new neurological mutant in the mouse by Doolittle D. P. (1977)
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{'key': 'e_1_2_2_22_1', 'first-page': '171', 'volume-title': 'Neurological Mutations Affecting Myelinalion, INSERM Symposium No. 14', 'author': 'Ganser A. L.', 'year': '1980'} / Neurological Mutations Affecting Myelinalion, INSERM Symposium No. 14 by Ganser A. L. (1980)
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10.1111/j.1471-4159.1970.tb03370.x
{'key': 'e_1_2_2_28_1', 'first-page': '27', 'volume-title': 'Neurological Mutations Affecting Myelination', 'author': 'Lachapelle F.', 'year': '1980'} / Neurological Mutations Affecting Myelination by Lachapelle F. (1980)
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{'key': 'e_1_2_2_37_1', 'first-page': '545', 'volume-title': 'Neurological Mutations Affecting Myelination. INSERM Symposium No. 14', 'author': 'Norton W. T.', 'year': '1980'} / Neurological Mutations Affecting Myelination. INSERM Symposium No. 14 by Norton W. T. (1980)
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10.1111/j.1471-4159.1979.tb04565.x

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 10:18 p.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 11:58 a.m.)
Issued	41 years, 6 months ago (March 1, 1984)
Published	41 years, 6 months ago (March 1, 1984)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	41 years, 6 months ago (March 1, 1984)

Funders 0

None

BibTeX

@article{Campagnoni_1984, title={Cell‐Free Synthesis of Myelin Basic Proteins in Normal and Dysmyelinating Mutant Mice}, volume={42}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1984.tb02744.x}, DOI={10.1111/j.1471-4159.1984.tb02744.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Campagnoni, A. T. and Campagnoni, C. W. and Bourre, J.‐M. and Jacque, C. and Baumann, N.}, year={1984}, month=mar, pages={733–739} }

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  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1984,
        3
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1984,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1471-4159.1984.tb02744.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1471-4159.1984.tb02744.x",
  "relation": {},
  "ISSN": [
    "0022-3042",
    "1471-4159"
  ],
  "subject": [],
  "container-title-short": "Journal of Neurochemistry",
  "published": {
    "date-parts": [
      [
        1984,
        3
      ]
    ]
  }
}