DOI: 10.1111/j.1471-4159.1983.tb13580.x. Protein Components of the Purified Sodium Channel from Rat Skeletal Muscle Sarcolemma

Protein Components of the Purified Sodium Channel from Rat Skeletal Muscle Sarcolemma

10.1111/j.1471-4159.1983.tb13580.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Sensitive detection systems have been used to study the protein components of the sodium channel purified from rat skeletal muscle sarcolemma. This functional, purified sodium channel contains at least three subunits on 7–20% gradient sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis: a large glycoprotein which migrates anomalously in the high‐molecular‐weight range, a 45,000 molecular weight polypeptide, and a third protein often seen as a doublet at 38,000. The large glycoprotein runs as a diffuse band and stains very poorly with Coomassie blue, but is adequately visualized with silver staining or iodination followed by autoradiography. This glycoprotein exhibits anomalous electrophoretic behavior in SDS‐polyacrylamide gels. The apparent molecular weight of the center of the band varies from ∼230,000 on 13% acrylamide gels to ∼130,000 on 5% gels; on 7–20% gradient gels a value of 160,000 is found. Plots of relative migration versus gel concentration suggest an unusually high apparent free solution mobility. Lectin binding to purified channel peptides separated by gel electrophoresis indicates that the large glycoprotein is the only subunit that binds either concanavalin A or wheat germ agglutinin, and this component has high binding capacity for both lectins. The smaller channel components run consistently at 45,000 and 38,000 molecular weight in a variety of gel systems and do not appear to be glycosylated.

Bibliography

Barchi, R. L. (1983). Protein Components of the Purified Sodium Channel from Rat Skeletal Muscle Sarcolemma. Journal of Neurochemistry, 40(5), 1377â1385. Portico.

Authors 1

Robert L. Barchi (first)

References 32 Referenced 118

10.1021/bi00577a010
10.1073/pnas.75.6.2606
10.1016/0006-291X(80)90782-2
10.1111/j.1471-4159.1981.tb10842.x
10.1016/0005-2736(79)90115-9
10.1073/pnas.77.3.1306
10.1073/pnas.77.1.639
{'key': 'e_1_2_7_9_1', 'first-page': '229', 'article-title': 'Major human erythrocyte glycoprotein spans the cell membrane', 'volume': '231', 'author': 'Bretscher M.', 'year': '1971', 'journal-title': 'Nature'} / Nature / Major human erythrocyte glycoprotein spans the cell membrane by Bretscher M. (1971)
10.1016/0076-6879(78)50007-4
10.1016/0005-2736(81)90196-6
10.1016/S0026-0495(64)80018-4
{'key': 'e_1_2_7_13_1', 'first-page': '6339', 'article-title': 'Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat', 'volume': '246', 'author': 'Glossmann H.', 'year': '1971', 'journal-title': 'J. Biol. Chem'} / J. Biol. Chem / Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat by Glossmann H. (1971)
10.1073/pnas.78.7.4620
10.1016/S0021-9258(19)70342-1 / J. Biol. Chem / Size characteristics of the solubilized saxitoxin receptor of the voltage‐sensitive sodium channel from rat brain by Hartshorne R. (1980)
10.1016/S0076-6879(77)46072-5
10.1038/227680a0
10.1021/bi00566a011
10.1016/0003-2697(79)90045-9
{'key': 'e_1_2_7_20_1', 'first-page': '179', 'volume-title': 'Methods in Virology, Vol. 5', 'author': 'Maizel J.', 'year': '1971'} / Methods in Virology, Vol. 5 by Maizel J. (1971)
10.1016/S0076-6879(81)72018-4
10.1146/annurev.bi.41.070172.003325
10.1126/science.6162199
{'key': 'e_1_2_7_24_1', 'first-page': '385a', 'article-title': 'Purification, composition and visualization of a TTX‐binding glycoprotein', 'volume': '37', 'author': 'Miller J.', 'year': '1982', 'journal-title': 'Biophys. J'} / Biophys. J / Purification, composition and visualization of a TTX‐binding glycoprotein by Miller J. (1982)
10.1016/0003-2697(81)90463-2
10.1113/jphysiol.1976.sp011569
10.1016/0076-6879(72)28007-7
10.1016/0006-291X(71)90612-7
10.1016/0003-2697(81)90131-7
10.1016/S0065-3233(08)60451-9
10.1016/S0021-9258(18)33644-5 / J. Biol. Chem / Reconstitution of neurotoxin‐stimulated sodium transport by the voltage‐sensitive sodium channel purified from rat brain by Talvenheimo J. (1982)
10.1016/0014-5793(78)81199-5
10.1073/pnas.79.11.3651

Dates

Type	When
Created	18 years, 8 months ago (Dec. 15, 2006, 9:20 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 11:10 a.m.)
Indexed	1 day, 4 hours ago (Sept. 4, 2025, 10:21 a.m.)
Issued	42 years, 4 months ago (May 1, 1983)
Published	42 years, 4 months ago (May 1, 1983)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	42 years, 4 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Barchi_1983, title={Protein Components of the Purified Sodium Channel from Rat Skeletal Muscle Sarcolemma}, volume={40}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1983.tb13580.x}, DOI={10.1111/j.1471-4159.1983.tb13580.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Barchi, Robert L.}, year={1983}, month=may, pages={1377–1385} }

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