DOI: 10.1111/j.1471-4159.1983.tb09047.x. Multiple Forms of Choline‐<i>O</i>‐Acetyltransferase in Mouse and Rat Brain: Solubilization and Characterization

Multiple Forms of Choline‐O‐Acetyltransferase in Mouse and Rat Brain: Solubilization and Characterization

10.1111/j.1471-4159.1983.tb09047.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Three forms of acetyl coenzyme A: choline‐O‐acetyltransferase (EC 2.3.1.6, ChAT) have been isolated from mouse and rat forebrain synaptosomes with a 100 mM sodium phosphate (NaP) buffer of pH 7.4, a high‐salt solution (500 mM NaCl), and a 2% Triton DN‐65 solution, respectively. The Triton‐solubilized form of ChAT differed from the other two forms in its capacity to acetylate homocholine, its pH profile, and its sensitivity to denaturation. NaCl‐solubilized ChAT could be distinguished from the other two forms with respect to pH profile, sensitivity to inhibition by 4–(1‐naphthylvinyl) pyridine (in the presence of Triton), and apparent Km value for choline acetylation. The caudate and putamen of rat brain contained the highest amount of ChAT activity, based on tissue wet weight, and the cerebellum contained the least of the brain regions examined; only the cerebellum had more membrane‐bound than soluble ChAT. Septal lesion reduced ChAT activity in the NaP‐ and Triton‐solubilized fractions prepared from hippocampus by 68% and 64%, respectively, whereas it reduced the activity of the NaCl‐solubilized fraction by only 21%. These results suggest that three different forms of ChAT may exist in both mouse and rat brain.

Bibliography

Benishin, C. G., & Carroll, P. T. (1983). Multiple Forms of CholineâOâAcetyltransferase in Mouse and Rat Brain: Solubilization and Characterization. Journal of Neurochemistry, 41(4), 1030â1039. Portico.

Authors 2

Christina G. Benishin (first)
Paul T. Carroll (additional)

References 32 Referenced 79

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 9:55 a.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 11:01 p.m.)
Indexed	1 month, 4 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	42 years, 5 months ago (April 1, 1983)
Published	42 years, 5 months ago (April 1, 1983)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	42 years, 5 months ago (April 1, 1983)

Funders 0

None

BibTeX

@article{Benishin_1983, title={Multiple Forms of Choline‐O‐Acetyltransferase in Mouse and Rat Brain: Solubilization and Characterization}, volume={41}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1983.tb09047.x}, DOI={10.1111/j.1471-4159.1983.tb09047.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Benishin, Christina G. and Carroll, Paul T.}, year={1983}, month=apr, pages={1030–1039} }

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