DOI: 10.1111/j.1471-4159.1983.tb00844.x. The Role of Phosphatidylserine Decarboxylase in Brain Phospholipid Metabolism

The Role of Phosphatidylserine Decarboxylase in Brain Phospholipid Metabolism

10.1111/j.1471-4159.1983.tb00844.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: In brain, phosphatidylethanolamine can be synthesized from free ethanolamine either by a pathway involving the formation of CDP‐ethanolamine and its transfer to diglyceride, or by base‐exchange of ethanolamine with existing phospholipids. Although de novo synthesis from serine has also been demonstrated, the metabolic pathway involved is not known. The enzyme phosphatidylserine decarboxylase appears to be involved in the synthesis of much of the phosphatidylethanolamine in liver, but the significance of this route in brain has been challenged. Our in vitro studies demonstrate the existence of phosphatidylserine decarboxylase activity in rat brain and characterize some of its properties. This enzyme is localized in the mitochondrial fraction, whereas the enzymes involved in base‐exchange and the cytidine pathway are localized to microsomal membranes. Parallel in vivo studies showed that after the intracranial injection of L‐[G‐3H] serine, the specific activity of phosphatidylserine was greater in the microsomal fractions than in the mitochondrial fraction, whereas the opposite was true for phosphatidylethanolamine. When L‐[U‐14C]serine and [13H]ethanolamine were simultaneously injected, the 14C/3H ratio in mitochondrial phosphatidylethanolamine was 10 times that in microsomal phosphatidylethanolamine. The results demonstrate that serine is incorporated into the base moiety of phosphatidylethanolamine primarily through the decarboxylation of phosphatidylserine in brain mitochondria. A minimal value of 7% for the contribution of phosphatidylserine decarboxylase to whole‐brain phosphatidylethanolamine synthesis can be estimated from the in vivo data.

Bibliography

Butler, M., & Morell, P. (1983). The Role of Phosphatidylserine Decarboxylase in Brain Phospholipid Metabolism. Journal of Neurochemistry, 41(5), 1445â1454. Portico.

Authors 2

Madeline Butler (first)
Pierre Morell (additional)

References 49 Referenced 42

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{'key': 'e_1_2_1_4_1', 'first-page': '377', 'volume-title': 'Form and Function of Phospholipids', 'author': 'Ansell G. B.', 'year': '1973'} / Form and Function of Phospholipids by Ansell G. B. (1973)
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{'key': 'e_1_2_1_8_1', 'first-page': '222', 'article-title': 'Identification and properties of methyltransferases that synthesize phosphatidylcholine in rat brain synaptosomes', 'volume': '13', 'author': 'Blusztajn J. K.', 'year': '1982', 'journal-title': 'Trans. Am. Soc. Neurochem'} / Trans. Am. Soc. Neurochem / Identification and properties of methyltransferases that synthesize phosphatidylcholine in rat brain synaptosomes by Blusztajn J. K. (1982)
{'key': 'e_1_2_1_9_1', 'first-page': '518', 'article-title': 'The choline cycle: Evidence for biosynthesis of choline from serine and methionine in rat brain', 'volume': '8', 'author': 'Blusztajn J. K.', 'year': '1982', 'journal-title': 'Soc. Neurosci. Abst'} / Soc. Neurosci. Abst / The choline cycle: Evidence for biosynthesis of choline from serine and methionine in rat brain by Blusztajn J. K. (1982)
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10.1016/0006-3002(60)90470-4
{'key': 'e_1_2_1_12_1', 'first-page': '156', 'article-title': 'Topographic localization of phospholipid synthesis on brain microsomes', 'volume': '12', 'author': 'Butler M.', 'year': '1981', 'journal-title': 'Trans. Am. Soc. Neurochem'} / Trans. Am. Soc. Neurochem / Topographic localization of phospholipid synthesis on brain microsomes by Butler M. (1981)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:01 a.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 9:18 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:48 a.m.)
Issued	42 years, 3 months ago (May 1, 1983)
Published	42 years, 3 months ago (May 1, 1983)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	42 years, 3 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Butler_1983, title={The Role of Phosphatidylserine Decarboxylase in Brain Phospholipid Metabolism}, volume={41}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1983.tb00844.x}, DOI={10.1111/j.1471-4159.1983.tb00844.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Butler, Madeline and Morell, Pierre}, year={1983}, month=may, pages={1445–1454} }

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