DOI: 10.1111/j.1471-4159.1982.tb10875.x. Cerebroside Sulfotransferase in Golgi‐Enriched Fractions from Rat Brain

Cerebroside Sulfotransferase in Golgi‐Enriched Fractions from Rat Brain

10.1111/j.1471-4159.1982.tb10875.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Golgi‐enriched fractions were prepared from brainstems of 17‐day‐old rats by first floating off myelin, then fractionating the remaining pellet by a series of differential and density gradient centrifugations in sucrose. Fractions enriched in Golgi membranes were recovered at 0.46/0.76 m and 0.76/0.87 m interfaces on the final sucrose gradient as indicated by morphology and the biochemical markers thiamine pyrophosphatase and [3H]fucose‐labeled glycoprotein. Morphology of the two fractions indicated very little contamination with myelin lamellae; however, the presence of significant levels of 2′, 3′‐cyclic nucleotidase in the lighter fraction suggested a contribution from oligodendroglial or myelin‐related membranes. Cerebroside sulfotransferase was highly enriched in the lighter Golgi‐enriched fraction relative to the denser fraction, the post‐34, 880 x g microsomes, and the myelin‐like fraction. In contrast, ceramide galactosyl transferase was more evenly distributed among the fractions. Our results show a more highly localized distribution of sulfatide synthesis than of galactocerebroside synthesis, probably in Golgi membranes or oligodendroglia‐related membranes with similar properties.

Bibliography

Benjamins, J. A., Hadden, T., & Skoff, R. P. (1982). Cerebroside Sulfotransferase in GolgiâEnriched Fractions from Rat Brain. Journal of Neurochemistry, 38(1), 233â241. Portico.

Authors 3

Joyce A. Benjamins (first)
Timothy Hadden (additional)
Robert P. Skoff (additional)

References 25 Referenced 53

10.1111/j.1471-4159.1979.tb04576.x
10.1111/j.1471-4159.1976.tb12283.x
10.1016/0005-2760(79)90043-2
10.1111/j.1471-4159.1979.tb11044.x
10.1083/jcb.31.1.181
{'key': 'e_1_2_2_7_1', 'first-page': '225', 'article-title': 'Functional aspects of the Golgi apparatus', 'volume': '1', 'author': 'Dauwalder M.', 'year': '1971', 'journal-title': 'Subcell Biochem.'} / Subcell Biochem. / Functional aspects of the Golgi apparatus by Dauwalder M. (1971)
10.1111/j.1471-4159.1970.tb03730.x
10.1016/0304-4165(78)90024-7
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10.1042/bj0920091
10.1016/S0021-9258(18)61991-X / J. Biol. Chem. / Characterization of cerebroside sulfotransferase from rat brain by Farrell D. F. (1971)
{'key': 'e_1_2_2_14_1', 'first-page': '180', 'volume-title': 'Methods in Enzymology', 'author': 'Fleischer B.', 'year': '1974'} / Methods in Enzymology by Fleischer B. (1974)
10.1002/jss.400070108
10.1016/S0021-9258(20)79917-5 / J. Biol. Chem. / Golgi apparatus of rat kidney. Preparation and role in sulfatide formation by Fleischer B. (1974)
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10.1016/S0021-9258(17)45217-3 / J. Biol. Chem. / Observations on cerebroside metabolism in vivo by Kanfer J. (1965)
10.1016/S0022-2275(20)39651-6 / J. Lipid Res. / In vivo conversions of cerebroside and ceramide in rat brain by Kopaczyk K. C. (1965)
{'key': 'e_1_2_2_20_1', 'first-page': '130', 'volume-title': 'Methods in Enzymology', 'author': 'Morre D. J.', 'year': '1971'} / Methods in Enzymology by Morre D. J. (1971)
10.1111/j.1471-4159.1973.tb07519.x
10.1016/S0021-9258(17)34906-2
10.1111/j.1471-4159.1979.tb05180.x
10.1002/cne.901690303
10.1083/jcb.32.2.415
10.1083/jcb.57.1.175

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:08 a.m.)
Deposited	1 year, 9 months ago (Nov. 4, 2023, 3:59 a.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 6:44 a.m.)
Issued	43 years, 7 months ago (Jan. 1, 1982)
Published	43 years, 7 months ago (Jan. 1, 1982)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	43 years, 7 months ago (Jan. 1, 1982)

Funders 0

None

BibTeX

@article{Benjamins_1982, title={Cerebroside Sulfotransferase in Golgi‐Enriched Fractions from Rat Brain}, volume={38}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1982.tb10875.x}, DOI={10.1111/j.1471-4159.1982.tb10875.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Benjamins, Joyce A. and Hadden, Timothy and Skoff, Robert P.}, year={1982}, month=jan, pages={233–241} }

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