DOI: 10.1111/j.1471-4159.1981.tb02377.x. Cleavage of Rabbit Myelin Basic Protein by Pepsin

Cleavage of Rabbit Myelin Basic Protein by Pepsin

10.1111/j.1471-4159.1981.tb02377.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract:Rapid cleavage of bovine and guinea pig myelin basic proteins by pepsin at pH 6.0 is limited to the Phe‐Phe bond in the middle of the molecule. In the rabbit protein, however, rapid cleavages occur elsewhere in addition to the Phe87‐Phe88 bond in regions in which there are amino acid substitutions. Rapid cleavage occurs at the Leu151‐Phe152 bond, at which Ile‐151 has been replaced by Leu, the residue that actually contributes the scissile bond. Rapid cleavages occur at the Phe44‐Phe45 and Leu109‐Ser110 bonds, which in the bovine and guinea pig proteins are relatively resistant under the experimental conditions (pH 6.0). The increased susceptibility of these bonds in the rabbit protein appears to be related to the replacement of Gly‐46 by Ser and the change in the sequence immediately NH2‐terminal to Leu‐109, from Leu‐Ser to Thr‐Val. These cleavages of the rabbit protein at the four very susceptible bonds have permitted us to isolate peptides (1‐44), (45‐87), (88‐109), (110‐151), and (152‐168) in high yield. We have also isolated peptides (88‐151), (1‐14), and (15‐44) in low yield; the latter two result from limited cleavage at the relatively resistant Tyr14Leu15 bond. Peptide (88‐109) has been chromatographically resolved into species differing in the degree of methylation of Arg‐105; this resolution is thought to result from differences in hydrogen bonding ability of the guanidinium groups.

Bibliography

Martenson, R. E., Liithy, V., & Deibler, G. E. (1981). Cleavage of Rabbit Myelin Basic Protein by Pepsin. Journal of Neurochemistry, 36(1), 58â68. Portico.

Authors 3

Russell E. Martenson (first)
Vivian Liithy (additional)
Gladys E. Deibler (additional)

References 32 Referenced 41

10.1016/0020-711X(71)90023-1
{'issue': '3', 'key': 'e_1_2_1_3_1', 'first-page': '2492', 'article-title': 'The base strengths of methylated guanidines', 'author': 'Angyal S. J.', 'year': '1951', 'journal-title': 'J. Chem. Soc. (London)'} / J. Chem. Soc. (London) / The base strengths of methylated guanidines by Angyal S. J. (1951)
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{'key': 'e_1_2_1_8_1', 'first-page': '45', 'article-title': 'Prediction of the secondary structure of proteins from their amino acid sequences', 'volume': '47', 'author': 'Chou P. Y.', 'year': '1978', 'journal-title': 'Adv. Enzymol.'} / Adv. Enzymol. / Prediction of the secondary structure of proteins from their amino acid sequences by Chou P. Y. (1978)
10.1016/S0021-9258(17)33540-8 / J. Biol. Chem. / Basis of microheterogeneity of myelin basic protein by Chou F. C.‐H. (1976)
10.1016/S0021-9258(19)44122-7 / J. Biol. Chem. / Chromatographic fractionation of myelin basic protein. Partial characterization and methylarginine contents of the multiple forms by Deibler G. E. (1973)
10.1080/00327487208061467
10.1016/S0021-9258(19)40905-8 / J. Biol. Chem. / The contribution of phosphorylation and loss of COOH‐terminal arginine to the microheterogeneity of myelin basic protein by Deibler G. E. (1975)
10.1126/science.184.4132.73
10.4049/jimmunol.117.1.110 / J. Immunol. / Protection against experimental allergic encephalomyelitis with peptides derived from myelin basic protein: Presence of intact encephalitogenic site is essential by Driscoll B. F. (1976)
10.4049/jimmunol.110.6.1699 / J. Immunol. / Encephalitogenic activity of peptides from the smaller basic protein of rat myelin by Dunkley P. R. (1973)
10.1016/0304-4165(65)90147-9
10.1126/science.168.3936.1220
{'key': 'e_1_2_1_18_1', 'first-page': '3418', 'article-title': 'Allergic encephalomyelitis. An encephalitogenic peptide derived from the basic protein of myelin', 'volume': '246', 'author': 'Eylar E. H.', 'year': '1971', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Allergic encephalomyelitis. An encephalitogenic peptide derived from the basic protein of myelin by Eylar E. H. (1971)
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{'key': 'e_1_2_1_20_1', 'first-page': '568', 'volume-title': 'Methods in Enzymol‐ogy', 'author': 'Hayashi R.', 'year': '1976'} / Methods in Enzymol‐ogy by Hayashi R. (1976)
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{'key': 'e_1_2_1_22_1', 'first-page': '1784', 'article-title': 'Specificity of rabbit antibodies to myelin basic protein', 'volume': '37', 'author': 'Kies M. W.', 'year': '1978', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Specificity of rabbit antibodies to myelin basic protein by Kies M. W. (1978)
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10.4049/jimmunol.114.2_Part_1.592 / J. Immunol. / The location of regions in guinea pig and bovine myelin basic proteins which induce experimental allergic encephalomyelitis in Lewis rats by Martenson R. E. (1975)
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10.4049/jimmunol.115.5.1456 / J. Immunol. / The immune response against an encephalitogenic fragment of guinea pig basic protein in the Lewis and Brown Norway strains of rat by McFarlin D. E. (1975)
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10.1016/S0021-9258(19)40699-6 / J. Biol. Chem. / Antigenic determinants of bovine myelin encephalitogenic protein recognized by rabbit antibody to myelin encephalitogenic protein by Whitaker J. N. (1975)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:53 a.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 2:54 p.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 11:59 a.m.)
Issued	44 years, 8 months ago (Jan. 1, 1981)
Published	44 years, 8 months ago (Jan. 1, 1981)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	44 years, 8 months ago (Jan. 1, 1981)

Funders 0

None

BibTeX

@article{Martenson_1981, title={Cleavage of Rabbit Myelin Basic Protein by Pepsin}, volume={36}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1981.tb02377.x}, DOI={10.1111/j.1471-4159.1981.tb02377.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Martenson, Russell E. and Liithy, Vivian and Deibler, Gladys E.}, year={1981}, month=jan, pages={58–68} }

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