DOI: 10.1111/j.1471-4159.1981.tb01616.x. Correlations Between Na<sup>+</sup>‐K<sup>+</sup> ATPase Activity and Acetylcholine Release in Rat Cortical Synaptosomes

Correlations Between Na+‐K+ ATPase Activity and Acetylcholine Release in Rat Cortical Synaptosomes

10.1111/j.1471-4159.1981.tb01616.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: [3H]Acetylcholine efflux and Na+‐K+ ATPase ion pump activity were measured concomitantly in rat cortical synaptosomes. Ouabain (500 μM), strophanthidin (500 μM). and parachloromercuribenzene sulfonate (500 μM) each inhibited ouabain‐sensitive 86Rb uptake and elevated [3H]acetylcholine release independently of the external calcium concentration. Veratridine (10 μM), electrical field stimulation (60 V, 60 Hz, 5‐ms pulse duration), or the calcium ionophore A23187 (10μg/ml) also inhibited ouabain‐sensitive 86Rb uptake and released [3H]acetylcholine, but via a calcium‐dependent process. Veratridine‐induced [3H]acetylcholine release and ion pump inhibition were correlated over a wide range of drug concentrations and both effects were blocked by pre‐treatment with tetrodotoxin (1 μM). The rate of [3H]acetyl‐choline efflux from superfused synaptosomes was increased within 15 s of exposure to ouabain, strophanthidin, veratridine, A23187, or field stimulation, while ouabain‐sensitive 86Rb uptake was significantly decreased within a similar interval. These results suggest that [3H]acetylcholine release is due at least in part to inhibition of Na+‐K+ ATPase.

Bibliography

Meyer, E. M., & Cooper, J. R. (1981). Correlations Between Na+âK+ ATPase Activity and Acetylcholine Release in Rat Cortical Synaptosomes. Journal of Neurochemistry, 36(2), 467â475. Portico.

Authors 2

E. M. Meyer (first)
J. R. Cooper (additional)

References 33 Referenced 103

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{'key': 'e_1_2_1_8_1', 'first-page': '824', 'article-title': 'Effects of ethanol on neurotransmitter release by rat brain cortical slices', 'volume': '193', 'author': 'Carmichael F. J.', 'year': '1975', 'journal-title': 'J. Pharmacol. Exp. Ther.'} / J. Pharmacol. Exp. Ther. / Effects of ethanol on neurotransmitter release by rat brain cortical slices by Carmichael F. J. (1975)
10.1016/S0021-9258(19)41385-9 / J. Biol. Chem. / Activation of the action potential Na+‐ionophore of cultured neuroblastoma cells by veratridine and batrachotoxin by Catterall W. A. (1975)
{'key': 'e_1_2_1_10_1', 'first-page': '135', 'volume-title': 'Neurotransmitter Function: Basic and Clinical Aspects', 'author': 'Cooper J. R.', 'year': '1977'} / Neurotransmitter Function: Basic and Clinical Aspects by Cooper J. R. (1977)
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{'key': 'e_1_2_1_12_1', 'first-page': '535', 'article-title': 'Influence of membrane functions on synthesis of acetylcholine increased by cholinomimetic drugs in slices of telencephalon of rats', 'volume': '32', 'author': 'Fischer H. D.', 'year': '1974', 'journal-title': 'Acta Biol. Med. Germ.'} / Acta Biol. Med. Germ. / Influence of membrane functions on synthesis of acetylcholine increased by cholinomimetic drugs in slices of telencephalon of rats by Fischer H. D. (1974)
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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 6:56 a.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 2:35 p.m.)
Indexed	2 days, 19 hours ago (Sept. 4, 2025, 10:24 a.m.)
Issued	44 years, 7 months ago (Feb. 1, 1981)
Published	44 years, 7 months ago (Feb. 1, 1981)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	44 years, 7 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{Meyer_1981, title={Correlations Between Na+‐K+ ATPase Activity and Acetylcholine Release in Rat Cortical Synaptosomes}, volume={36}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1981.tb01616.x}, DOI={10.1111/j.1471-4159.1981.tb01616.x}, number={2}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Meyer, E. M. and Cooper, J. R.}, year={1981}, month=feb, pages={467–475} }

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