DOI: 10.1111/j.1471-4159.1979.tb11730.x. DIFFERENTIAL ENRICHMENT OF CELLS FROM EMBRYONIC RAT CEREBRA BY CENTRIFUGAL ELUTRIATION

DIFFERENTIAL ENRICHMENT OF CELLS FROM EMBRYONIC RAT CEREBRA BY CENTRIFUGAL ELUTRIATION

10.1111/j.1471-4159.1979.tb11730.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract—Centrifugal elutriation was used to obtain different populations of cells dissociated from 16‐day‐old rat embryo cerebra. The cell populations recovered were viable and could be maintained for several weeks in vitro. Sterile conditions were maintained throughout a preparation. Rat pups were removed by Caesarean section, the cerebra dissected and the cells dissociated by brief exposure to trypsin (0.125%, 6 min). An equivalent volume of elutriation medium (Dulbecco's medium containing 1% fetal calf serum, sodium bicarbonate, penicillin and streptomycin, EDTA, and deoxyribonuclease) was added to the trypsin‐cell suspension, the dissociated cells pelleted, resuspended in elutriation medium and counted. Up to 4 x 108 cells were injected into the previously sterilized elutriator. Seven fractions were usually recovered from a preparation. The first fraction contained primarily red blood cells and cell debris, which could not be maintained in vitro. Upon culture, fraction 2 consisted of predominantly non‐neuronal cells, while fractions 3–6 contained neuronal and non‐neuronal cells. The morphological characteristics of the neurons differed in these fractions. Fraction 7 contained cells that had reaggregated during the elutriation procedure and exhibited a variety of cell types in vitro.

Bibliography

Schengrund, C., & Repman, M. A. (1979). DIFFERENTIAL ENRICHMENT OF CELLS FROM EMBRYONIC RAT CEREBRA BY CENTRIFUGAL ELUTRIATION. Journal of Neurochemistry, 33(1), 283â289. Portico.

Authors 2

Cara‐Lynne Schengrund (first)
Mary Ann Repman (additional)

References 29 Referenced 7

10.1016/0003-2697(73)90465-X
10.1002/jcp.1040810215
{'key': 'e_1_2_1_4_1', 'first-page': '134', 'article-title': 'Reconstituted rat tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tubes', 'volume': '7', 'author': 'Bornstein M. B.', 'year': '1958', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Reconstituted rat tail collagen used as substrate for tissue cultures on coverslips in maximow slides and roller tubes by Bornstein M. B. (1958)
10.1080/00327487408068770
10.1016/S0021-9258(18)55986-X
{'key': 'e_1_2_1_7_1', 'first-page': '1', 'volume-title': 'Fractions 1', 'author': 'Grabske R.', 'year': '1978'} / Fractions 1 by Grabske R. (1978)
10.1016/0003-2697(78)90573-0
10.1111/j.1471-4159.1976.tb04433.x
10.1016/B978-0-12-250450-1.50037-5
{'key': 'e_1_2_1_11_1', 'first-page': '117', 'volume-title': 'Developmental Neurobiology', 'author': 'Lavelle A.', 'year': '1970'} / Developmental Neurobiology by Lavelle A. (1970)
10.1016/S0021-9258(19)52451-6
10.1083/jcb.59.2.329
10.1016/0006-8993(78)90795-3
{'key': 'e_1_2_1_15_1', 'first-page': '4291', 'article-title': 'Separation of cells from mouse solid tumors by centrifugal elutriation', 'volume': '37', 'author': 'Meistrich M. L.', 'year': '1977', 'journal-title': 'Cancer Res.'} / Cancer Res. / Separation of cells from mouse solid tumors by centrifugal elutriation by Meistrich M. L. (1977)
{'key': 'e_1_2_1_16_1', 'first-page': '561', 'volume-title': 'Methods in Enzymology, Vol. 35, part B', 'author': 'Poduslo S. E.', 'year': '1975'} / Methods in Enzymology, Vol. 35, part B by Poduslo S. E. (1975)
10.1016/0006-8993(77)90709-0
10.1111/j.1471-4159.1977.tb10737.x
10.1016/S0021-9258(19)43786-1
{'key': 'e_1_2_1_20_1', 'first-page': '765', 'volume-title': 'Methods in Enzymology, Vol. 32, Part B', 'author': 'Schrier B. K.', 'year': '1974'} / Methods in Enzymology, Vol. 32, Part B by Schrier B. K. (1974)
10.1016/S0021-9258(19)41203-9 / J. biol. Chem. / Expression of differentiated activities in reaggregated brain cell cultures by Seeds N. W. (1975)
10.1016/B978-0-12-250450-1.50014-4
10.1016/0006-3002(57)90254-8
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10.1016/0006-8993(69)90065-1
{'key': 'e_1_2_1_27_1', 'first-page': '175', 'article-title': 'Dünnschichtchromatographie von Phosphatiden und Glykolipiden', 'volume': '234', 'author': 'Wagner H.', 'year': '1961', 'journal-title': 'Biochem. Z.'} / Biochem. Z. / Dünnschichtchromatographie von Phosphatiden und Glykolipiden by Wagner H. (1961)
10.1083/jcb.62.2.540
10.2307/3225969
10.1016/0014-4827(78)90048-4

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 5:52 a.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 12:36 p.m.)
Indexed	9 months, 2 weeks ago (Nov. 19, 2024, 11:02 a.m.)
Issued	46 years, 2 months ago (July 1, 1979)
Published	46 years, 2 months ago (July 1, 1979)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	46 years, 2 months ago (July 1, 1979)

Funders 0

None

BibTeX

@article{Schengrund_1979, title={DIFFERENTIAL ENRICHMENT OF CELLS FROM EMBRYONIC RAT CEREBRA BY CENTRIFUGAL ELUTRIATION}, volume={33}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1979.tb11730.x}, DOI={10.1111/j.1471-4159.1979.tb11730.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Schengrund, Cara‐Lynne and Repman, Mary Ann}, year={1979}, month=jul, pages={283–289} }

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