DOI: 10.1111/j.1471-4159.1978.tb06255.x. MECHANISM OF EXTRACELLULAR POTASSIUM STIMULATION OF PROTEIN SYNTHESIS IN THE IN VITRO HIPPOCAMPUS<sup>1</sup>

MECHANISM OF EXTRACELLULAR POTASSIUM STIMULATION OF PROTEIN SYNTHESIS IN THE IN VITRO HIPPOCAMPUS1

10.1111/j.1471-4159.1978.tb06255.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractTransverse slices of guinea‐pig hippocampus respond to small increases in extracellular potassium concentration [K +] by showing a marked augmentation in the rate of incorporation of labelled precursor amino acid into protein. We find that this augmentation is manifested equally in superfusing solutions containing low specific activity/high concentration precursor and high specific activity/low concentration precursor. This provides additional evidence that the effect of extracellular [K +] is on the rate of protein synthesis and not on the precursor pool. Extracellular [K +] augments amino acid incorporation normally in the presence of concentrations of α‐amanatin and actinomycin D which are inhibiting RNA synthesis by over 97%. This suggests the effect is exerted post‐transcriptionally. Extracellular [K +] causes an unattenuated increase in protein synthesis in the presence of high Mg2+ (20mm) or low Ca2+ (0 mm) in the superfusate–suggesting this effect of [K +] does not result from an effect on neurotransmitter release. Increases in extracellular [K+] which increase protein synthesis threefold (1.3‐6.4mm‐K +) increase the intracellular [K +]/[Na +] 2‐fold. 3 x 10‐7m‐Ouabain. which lowers incorporation by 40‐50% lowers [K +]/[Na +] by about 50% while having no effect on the uptake of labelled precursor or on the cell concentrations of ATP. These results suggest that protein synthesis in the cell is sensitive to [K. +]/[Na +] and that an alteration in this ratio is the mechanism by which the concentration of extracellular [K +] affects the rate of in vitro hippocampal protein synthesis. This effect of extracellular [K +] on synthesis is not observed in slices from non‐cerebral organs of the guinea pig.

Bibliography

Lipton, P., & Heimbach, C. J. (1978). MECHANISM OF EXTRACELLULAR POTASSIUM STIMULATION OF PROTEIN SYNTHESIS IN THE IN VITRO HIPPOCAMPUS1. Journal of Neurochemistry, 31(5), 1299â1307. Portico.

Authors 2

Peter Lipton (first)
Catherine J. Heimbach (additional)

References 42 Referenced 25

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10.1152/jn.1973.36.5.855
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10.1042/bj1040524
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10.1016/S0021-9258(17)40350-4 / J. biol. Chem. / Inhibition of protein synthesis by Cl‐ by Weber L. A. (1977)
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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 4:30 a.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 3:03 p.m.)
Indexed	1 year, 10 months ago (Nov. 4, 2023, 3:40 p.m.)
Issued	46 years, 10 months ago (Nov. 1, 1978)
Published	46 years, 10 months ago (Nov. 1, 1978)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	46 years, 10 months ago (Nov. 1, 1978)

Funders 0

None

BibTeX

@article{Lipton_1978, title={MECHANISM OF EXTRACELLULAR POTASSIUM STIMULATION OF PROTEIN SYNTHESIS IN THE IN VITRO HIPPOCAMPUS1}, volume={31}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1978.tb06255.x}, DOI={10.1111/j.1471-4159.1978.tb06255.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Lipton, Peter and Heimbach, Catherine J.}, year={1978}, month=nov, pages={1299–1307} }

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