DOI: 10.1111/j.1471-4159.1978.tb06251.x. CEREBRAL METABOLIC AND CIRCULATORY CHANGES INDUCED BY HYPOXIA IN STARVED RATS

CEREBRAL METABOLIC AND CIRCULATORY CHANGES INDUCED BY HYPOXIA IN STARVED RATS

10.1111/j.1471-4159.1978.tb06251.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractIn order to study cerebral metabolic and circulatory effects of hypoxia under conditions of restricted glucose supply, the arterial Po2, was reduced to 25–30mm Hg in artificially ventilated and lightly anaesthetized rats that were starved for 24 or 48 h prior to experiments. Arterial glucose concentrations, that were initially around 6μmol g‐1, were significantly reduced after 15min of hypoxia, and decreased to 50o of control after 30min.In animals studied after 30min of hypoxia (24 h of starvation), cerebral blood flow had increased 4‐fold and there was a moderate (25%) rise in cerebral oxygen consumption. During the course of hypoxia, cerebral cortical concentrations of glucose fell to low values. In spite of this, concentrations of pyruvate and lactate rose with time, and the sum of citric acid cycle intermediates (citrate, α‐ketoglutarate, fumarate. malate and oxaloacetate) increased. Changes in amino acids were dominated by a fall in aspartate and a rise in alanine concentration. There was a moderate reduction in phosphocreatine and a slight rise in ADP concentration, but concentrations at ATP and AMP were unchanged. The changes observed are similar to those previously obtained in fed animals. It is concluded that even if blood glucose concentrations fall to 3μmol g‐1, and cerebral energy flux is maintained, substrate supply is sufficient to cover the energy requirements of the tissue.Hypoxia was accompanied by increases in the lactate/pyruvate and β‐hydroxybutyrate acetoacetate ratios of blood. In the tissue, NADH/NAD+ ratios derived from the lactate, malate and β‐hydroxybutyrate dehydrogenase systems rose, while that derived from the glutamate dehydrogenase reaction fell. It is concluded that the latter system is not well suited for estimating mitochondrial redox changes in brain tissue.

Bibliography

Berntman, L., & Siesjou, B. K. (1978). CEREBRAL METABOLIC AND CIRCULATORY CHANGES INDUCED BY HYPOXIA IN STARVED RATS. Journal of Neurochemistry, 31(5), 1265â1276. Portico.

Authors 2

L. Berntman (first)
B. K. Siesjou (additional)

References 42 Referenced 19

10.1021/bi00851a033
10.1111/j.1471-4159.1974.tb07605.x
{'key': 'e_1_2_2_4_1', 'article-title': 'Cerebral oxygen consumption and blood flow in hypoxia: influence of sympathoadrenal activation', 'author': 'Berntman L.', 'journal-title': 'Stroke'} / Stroke / Cerebral oxygen consumption and blood flow in hypoxia: influence of sympathoadrenal activation by Berntman L.
{'key': 'e_1_2_2_5_1', 'article-title': 'Influence of extreme hypercapnia on cerebral blood flow and oxygen consumption in the rat brain', 'author': 'Berntman L.', 'journal-title': 'Anesthesial'} / Anesthesial / Influence of extreme hypercapnia on cerebral blood flow and oxygen consumption in the rat brain by Berntman L.
10.1152/jappl.1967.23.5.726
{'key': 'e_1_2_2_7_1', 'article-title': 'Effects of bicuculline‐induced seizures on cerebral metabolism and circulation of rats rendered hypoglycemic by starvation', 'author': 'Blennow G.', 'journal-title': 'Ann Neurol'} / Ann Neurol / Effects of bicuculline‐induced seizures on cerebral metabolism and circulation of rats rendered hypoglycemic by starvation by Blennow G.
{'key': 'e_1_2_2_8_1', 'first-page': '375', 'article-title': 'Polarographic determination of total oxygen content in small blood samples', 'volume': '34', 'author': 'Borgström L.', 'year': '1974', 'journal-title': 'J. clin. Invest.'} / J. clin. Invest. / Polarographic determination of total oxygen content in small blood samples by Borgström L. (1974)
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10.1111/j.1471-4159.1972.tb01417.x
{'key': 'e_1_2_2_13_1', 'first-page': '32', 'article-title': 'Eine schnelle Micromethode zur serienmassigen Bestimmung der O2‐Konzentration im Blut', 'volume': '281', 'author': 'Fabel H.', 'year': '1964', 'journal-title': 'Pflügers Arch. ges. Physiol.'} / Pflügers Arch. ges. Physiol. / Eine schnelle Micromethode zur serienmassigen Bestimmung der O2‐Konzentration im Blut by Fabel H. (1964)
10.1111/j.1471-4159.1975.tb04350.x
10.1001/archneurpsyc.1944.02290290059010
{'key': 'e_1_2_2_16_1', 'first-page': '328', 'article-title': 'Hypoglycemia in the infant rat', 'volume': '133', 'author': 'Himwktch E.', 'year': '1941', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. / Hypoglycemia in the infant rat by Himwktch E. (1941)
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{'key': 'e_1_2_2_24_1', 'volume-title': 'A Flexible System of Enzymatic Analysis', 'author': 'Lowry O. H.', 'year': '1972'} / A Flexible System of Enzymatic Analysis by Lowry O. H. (1972)
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10.1152/ajplegacy.1967.212.2.367

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:30 a.m.)
Deposited	1 year, 9 months ago (Nov. 4, 2023, 3:03 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 9, 2024, 1:56 a.m.)
Issued	46 years, 10 months ago (Nov. 1, 1978)
Published	46 years, 10 months ago (Nov. 1, 1978)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	46 years, 10 months ago (Nov. 1, 1978)

Funders 0

None

BibTeX

@article{Berntman_1978, title={CEREBRAL METABOLIC AND CIRCULATORY CHANGES INDUCED BY HYPOXIA IN STARVED RATS}, volume={31}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1978.tb06251.x}, DOI={10.1111/j.1471-4159.1978.tb06251.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Berntman, L. and Siesjou, B. K.}, year={1978}, month=nov, pages={1265–1276} }

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