DOI: 10.1111/j.1471-4159.1973.tb04224.x. BIOSYNTHESIS OF CHOLEST‐5‐ENE‐3β, 24‐DIOL (CEREBROSTEROL) BY BOVINE CEREBRAL CORTICAL MICROSOMES<sup>1</sup>

BIOSYNTHESIS OF CHOLEST‐5‐ENE‐3β, 24‐DIOL (CEREBROSTEROL) BY BOVINE CEREBRAL CORTICAL MICROSOMES1

10.1111/j.1471-4159.1973.tb04224.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractThe presence of cholest‐5‐ene‐3β, 24‐diol (cerebrosterol) in samples of human, bovine, and rabbit brains has been established by isolation of the sterol therefrom and identification by comparison of physical properties. Cholest‐5‐ene‐3β, 24‐diol was present at the level of 66.5 sg/g of dried tissue in human brain, 42.9 μg/g in cattle brain, and 89.5 pg/g in rabbit brain. Cholest‐5‐ene‐3β, 24‐diol was the only readily detectable hydroxycholesterol derivative in these brain tissues and was concentrated in the 105,000 g pellet (microsomal fraction) of both human and bovine cerebral cortex, with no demonstrable amounts of the sterol present in nuclear or mitochondrial fractions. Incubation of [1,2‐3H]‐ or of [4‐14C]‐cholesterol with the 105,000 g microsomal pellet from bovine cerebral cortical homogenates demonstrated 0.1‐0.38 per cent conversions to radioactive cholest‐5‐ene‐3β, 24‐diol, isolated and purified as the 3β, 24‐dibenzoate. The bioconversion required oxygen, and a stimulation of hydroxylation by added NADPH2 was demonstrated. Our observations establish that a sterol 24‐hydroxylase system is present in bovine cerebral cortex.

Bibliography

Dhar, A. K., Teng, J. I., & Smith, L. L. (1973). BIOSYNTHESIS OF CHOLESTâ5âENEâ3Î², 24âDIOL (CEREBROSTEROL) BY BOVINE CEREBRAL CORTICAL MICROSOMES1. Journal of Neurochemistry, 21(1), 51â60. Portico.

Authors 3

A. K. Dhar (first)
J. I. Teng (additional)
Leland L. Smith (additional)

References 50 Referenced 38

10.1016/0005-2760(66)90055-5
10.1016/0021-9150(71)90025-6
10.1111/j.1471-4159.1962.tb07489.x
{'key': 'e_1_2_1_5_1', 'first-page': '1351', 'volume': '29', 'author': 'DiFrlsco S.', 'year': '1953', 'journal-title': 'Boll. Soc. ital. Biol. sper.'} / Boll. Soc. ital. Biol. sper. by DiFrlsco S. (1953)
{'key': 'e_1_2_1_6_1', 'first-page': '720', 'volume': '83', 'author': 'Ercoli A.', 'year': '1953', 'journal-title': 'Gazz. chim. ital.'} / Gazz. chim. ital. by Ercoli A. (1953)
10.1021/ja01109a515
{'key': 'e_1_2_1_8_1', 'first-page': '738', 'volume': '83', 'author': 'Ercoli A.', 'year': '1953', 'journal-title': 'Gazz. chim. ital.'} / Gazz. chim. ital. by Ercoli A. (1953)
{'key': 'e_1_2_1_9_1', 'first-page': '494', 'volume': '29', 'author': 'Ercoli A.', 'year': '1953', 'journal-title': 'Boll. Soc. ital. Biol. sper.'} / Boll. Soc. ital. Biol. sper. by Ercoli A. (1953)
{'key': 'e_1_2_1_10_1', 'first-page': '78', 'volume': '83', 'author': 'Ercoli A.', 'year': '1953', 'journal-title': 'Gazz. chim. ital.'} / Gazz. chim. ital. by Ercoli A. (1953)
10.1021/ja01114a003
10.1021/ja01114a006
10.1021/jo01362a021
10.1016/0024-3205(71)90221-9
10.1126/science.162.3861.1495
{'key': 'e_1_2_1_16_1', 'first-page': '17', 'volume-title': 'Bile Salts', 'author': 'Haslewood G. A. D.', 'year': '1967'} / Bile Salts by Haslewood G. A. D. (1967)
10.1016/S0022-2275(20)39525-0 / J. Lipid Res. by Kamel A. M. (1971)
10.1016/0021-9797(71)90200-1
10.1083/jcb.30.2.405
{'key': 'e_1_2_1_20_1', 'first-page': 'III', 'volume': '42', 'author': 'Manzur F. N.', 'year': '1970', 'journal-title': 'Circulation'} / Circulation by Manzur F. N. (1970)
10.1007/BF02533198
10.1016/S0022-2275(20)42655-0
10.1016/S0039-128X(70)80115-5
10.1007/BF02532752
10.1007/BF02636893
10.1212/WNL.14.6.549
10.1515/bchm2.1932.204.1-2.13
{'key': 'e_1_2_1_28_1', 'first-page': '321', 'volume': '93', 'author': 'Polyakova N. M.', 'year': '1953', 'journal-title': 'Dok. Akad. Nauk. S.S.S.R.'} / Dok. Akad. Nauk. S.S.S.R. by Polyakova N. M. (1953)
10.1007/BF02533029
10.1111/j.1471-4159.1971.tb00011.x
10.1111/j.1471-4159.1972.tb01258.x
10.1515/bchm2.1969.350.2.1213
{'key': 'e_1_2_1_33_1', 'first-page': '93', 'volume': '192', 'author': 'Schönheimer R.', 'year': '1930', 'journal-title': "Hoppe-Seyler's Z. physiol. Chem."} / Hoppe-Seyler's Z. physiol. Chem. by Schönheimer R. (1930)
10.1515/bchm2.1930.192.1-3.73
10.1515/bchm2.1961.326.1.235
10.1016/0014-5793(72)80020-6
10.1016/S0021-9673(01)85851-9
10.1111/j.1471-4159.1972.tb01406.x
10.1016/0021-9150(70)90078-X
{'key': 'e_1_2_1_40_1', 'first-page': '37', 'volume': '31', 'author': 'Smith L. L.', 'year': '1973', 'journal-title': 'Texas Reports Biol. Med.'} / Texas Reports Biol. Med. by Smith L. L. (1973)
{'key': 'e_1_2_1_41_1', 'first-page': '51P', 'volume': '99', 'author': 'Steel G.', 'year': '1966', 'journal-title': 'Biochem. J.'} / Biochem. J. by Steel G. (1966)
10.1021/jo00966a043
10.1021/bi00862a037
10.1016/S0021-9673(01)92905-X
10.1016/0003-2697(68)90147-4
{'key': 'e_1_2_1_46_1', 'first-page': '167', 'volume': '27', 'author': 'Lier J. E.', 'year': '1969', 'journal-title': 'Texas Reports Biol. Med.'} / Texas Reports Biol. Med. by Lier J. E. (1969)
10.1016/S0021-9673(00)93677-X
10.1021/jo00833a031
10.1007/BF02531321
10.1021/jo00806a038
10.1111/j.1471-4159.1968.tb08955.x

Dates

Type	When
Created	18 years, 10 months ago (Oct. 4, 2006, 11:35 p.m.)
Deposited	1 year, 9 months ago (Nov. 4, 2023, 7:37 a.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 5:43 p.m.)
Issued	52 years, 1 month ago (July 1, 1973)
Published	52 years, 1 month ago (July 1, 1973)
Published Online	18 years, 10 months ago (Oct. 4, 2006)
Published Print	52 years, 1 month ago (July 1, 1973)

Funders 0

None

BibTeX

@article{Dhar_1973, title={BIOSYNTHESIS OF CHOLEST‐5‐ENE‐3β, 24‐DIOL (CEREBROSTEROL) BY BOVINE CEREBRAL CORTICAL MICROSOMES1}, volume={21}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1973.tb04224.x}, DOI={10.1111/j.1471-4159.1973.tb04224.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Dhar, A. K. and Teng, J. I. and Smith, Leland L.}, year={1973}, month=jul, pages={51–60} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        14
      ]
    ],
    "date-time": "2024-01-14T22:43:56Z",
    "timestamp": 1705272236846
  },
  "reference-count": 50,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            10,
            4
          ]
        ],
        "date-time": "2006-10-04T00:00:00Z",
        "timestamp": 1159920000000
      },
      "content-version": "vor",
      "delay-in-days": 12148,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The presence of cholest\u20105\u2010ene\u20103\u03b2, 24\u2010diol (cerebrosterol) in samples of human, bovine, and rabbit brains has been established by isolation of the sterol therefrom and identification by comparison of physical properties. Cholest\u20105\u2010ene\u20103\u03b2, 24\u2010diol was present at the level of 66.5 sg/g of dried tissue in human brain, 42.9 \u03bcg/g in cattle brain, and 89.5 pg/g in rabbit brain. Cholest\u20105\u2010ene\u20103\u03b2, 24\u2010diol was the only readily detectable hydroxycholesterol derivative in these brain tissues and was concentrated in the 105,000 g pellet (microsomal fraction) of both human and bovine cerebral cortex, with no demonstrable amounts of the sterol present in nuclear or mitochondrial fractions. Incubation of [1,2\u2010<jats:sup>3</jats:sup>H]\u2010 or of [4\u2010<jats:sup>14</jats:sup>C]\u2010cholesterol with the 105,000 g microsomal pellet from bovine cerebral cortical homogenates demonstrated 0.1\u20100.38 per cent conversions to radioactive cholest\u20105\u2010ene\u20103\u03b2, 24\u2010diol, isolated and purified as the 3\u03b2, 24\u2010dibenzoate. The bioconversion required oxygen, and a stimulation of hydroxylation by added NADPH<jats:sub>2</jats:sub> was demonstrated. Our observations establish that a sterol 24\u2010hydroxylase system is present in bovine cerebral cortex.</jats:p>",
  "DOI": "10.1111/j.1471-4159.1973.tb04224.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T03:35:47Z",
    "timestamp": 1160019347000
  },
  "page": "51-60",
  "source": "Crossref",
  "is-referenced-by-count": 38,
  "title": "BIOSYNTHESIS OF CHOLEST\u20105\u2010ENE\u20103\u03b2, 24\u2010DIOL (CEREBROSTEROL) BY BOVINE CEREBRAL CORTICAL MICROSOMES<sup>1</sup>",
  "prefix": "10.1111",
  "volume": "21",
  "author": [
    {
      "given": "A. K.",
      "family": "Dhar",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J. I.",
      "family": "Teng",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Leland L.",
      "family": "Smith",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        10,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2760(66)90055-5"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0021-9150(71)90025-6"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1962.tb07489.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "1351",
      "volume": "29",
      "author": "DiFrlsco S.",
      "year": "1953",
      "journal-title": "Boll. Soc. ital. Biol. sper."
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "720",
      "volume": "83",
      "author": "Ercoli A.",
      "year": "1953",
      "journal-title": "Gazz. chim. ital."
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja01109a515"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "738",
      "volume": "83",
      "author": "Ercoli A.",
      "year": "1953",
      "journal-title": "Gazz. chim. ital."
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "494",
      "volume": "29",
      "author": "Ercoli A.",
      "year": "1953",
      "journal-title": "Boll. Soc. ital. Biol. sper."
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "78",
      "volume": "83",
      "author": "Ercoli A.",
      "year": "1953",
      "journal-title": "Gazz. chim. ital."
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja01114a003"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja01114a006"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jo01362a021"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0024-3205(71)90221-9"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.162.3861.1495"
    },
    {
      "key": "e_1_2_1_16_1",
      "first-page": "17",
      "volume-title": "Bile Salts",
      "author": "Haslewood G. A. D.",
      "year": "1967"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "155",
      "DOI": "10.1016/S0022-2275(20)39525-0",
      "volume": "12",
      "author": "Kamel A. M.",
      "year": "1971",
      "journal-title": "J. Lipid Res."
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0021-9797(71)90200-1"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.30.2.405"
    },
    {
      "key": "e_1_2_1_20_1",
      "first-page": "III",
      "volume": "42",
      "author": "Manzur F. N.",
      "year": "1970",
      "journal-title": "Circulation"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02533198"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2275(20)42655-0"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0039-128X(70)80115-5"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02532752"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02636893"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1212/WNL.14.6.549"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1932.204.1-2.13"
    },
    {
      "key": "e_1_2_1_28_1",
      "first-page": "321",
      "volume": "93",
      "author": "Polyakova N. M.",
      "year": "1953",
      "journal-title": "Dok. Akad. Nauk. S.S.S.R."
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02533029"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1971.tb00011.x"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1972.tb01258.x"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1969.350.2.1213"
    },
    {
      "key": "e_1_2_1_33_1",
      "first-page": "93",
      "volume": "192",
      "author": "Sch\u00f6nheimer R.",
      "year": "1930",
      "journal-title": "Hoppe-Seyler's Z. physiol. Chem."
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1930.192.1-3.73"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1961.326.1.235"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(72)80020-6"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(01)85851-9"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1972.tb01406.x"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0021-9150(70)90078-X"
    },
    {
      "key": "e_1_2_1_40_1",
      "first-page": "37",
      "volume": "31",
      "author": "Smith L. L.",
      "year": "1973",
      "journal-title": "Texas Reports Biol. Med."
    },
    {
      "key": "e_1_2_1_41_1",
      "first-page": "51P",
      "volume": "99",
      "author": "Steel G.",
      "year": "1966",
      "journal-title": "Biochem. J."
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jo00966a043"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00862a037"
    },
    {
      "key": "e_1_2_1_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(01)92905-X"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(68)90147-4"
    },
    {
      "key": "e_1_2_1_46_1",
      "first-page": "167",
      "volume": "27",
      "author": "Lier J. E.",
      "year": "1969",
      "journal-title": "Texas Reports Biol. Med."
    },
    {
      "key": "e_1_2_1_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9673(00)93677-X"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jo00833a031"
    },
    {
      "key": "e_1_2_1_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02531321"
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jo00806a038"
    },
    {
      "key": "e_1_2_1_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1968.tb08955.x"
    }
  ],
  "container-title": "Journal of Neurochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.1973.tb04224.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.1973.tb04224.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        4
      ]
    ],
    "date-time": "2023-11-04T11:37:21Z",
    "timestamp": 1699097841000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1973.tb04224.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1973,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1471-4159.1973.tb04224.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1471-4159.1973.tb04224.x",
  "relation": {},
  "ISSN": [
    "0022-3042",
    "1471-4159"
  ],
  "subject": [],
  "container-title-short": "Journal of Neurochemistry",
  "published": {
    "date-parts": [
      [
        1973,
        7
      ]
    ]
  }
}