DOI: 10.1111/j.1471-4159.1972.tb01418.x. QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREON‐12<sup>1</sup>

QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREON‐121

10.1111/j.1471-4159.1972.tb01418.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract— Murine brains were frozen in situ, either in liquid N2 or in Freon‐12 cooled to its freezing point. The effect of these coolants on cooling rates and times in various CNS regions was determined. In addition, levels of ATP, P‐creatine and lactate were measured in selected regions of brains from both intact animals and severed heads frozen in either coolant. For both the intact animals and severed heads, superficial regions of brain cooled to 0°C and deeper regions to 25°C, at the same rate in either liquid N2 or Freon. Subsequent cooling was more rapid in liquid N2 in both regions. Levels of ATP, P‐creatine and lactate were similar in brains frozen in either coolant, probably because CNS utilization of highenergy phosphates decreased markedly as body temperature fell. In brains of animals frozen intact, levels of ATP and P‐creatine were higher and levels of lactate were lower than those in brains from heads which were severed prior to freezing. This difference may be a result of the marked stimulation which accompanies decapitation and may also reflect continued cerebral circulation in the intact animal for a brief time after immersing the animal in the coolant.

Bibliography

Ferrendelli, J. A., Gay, M. H., Sedgwick, W. G., & Chang, M. M. (1972). QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREONâ121. Journal of Neurochemistry, 19(4), 979â987. Portico.

Authors 4

J. A. Ferrendelli (first)
M. H. Gay (additional)
W. G. Sedgwick (additional)
M. M. Chang (additional)

References 21 Referenced 80

10.1111/j.1471-4159.1971.tb00003.x
{'key': 'e_1_2_1_3_1', 'first-page': '1', 'volume-title': 'Physical Techniques in Biological Research', 'author': 'Bell L. G. E.', 'year': '1956'} / Physical Techniques in Biological Research by Bell L. G. E. (1956)
10.1021/i260002a001
10.1016/0006-2952(68)90225-6
10.1111/j.1471-4159.1971.tb00219.x
10.1111/j.1471-4159.1969.tb05937.x
10.1111/j.1471-4159.1970.tb03733.x
10.1016/S0021-9258(18)75097-7
10.1042/bj0231126
10.1016/S0021-9258(18)51740-3
{'key': 'e_1_2_1_12_1', 'first-page': '267', 'volume-title': 'Handbook of Neurochemistry', 'author': 'Maker H. S.', 'year': '1971'} / Handbook of Neurochemistry by Maker H. S. (1971)
10.1152/ajplegacy.1925.75.1.70 / Am. J. Physiol. by McGinty D. A. (1925)
10.1177/12.10.777
{'key': 'e_1_2_1_15_1', 'first-page': '444', 'volume-title': 'Head Injury Conference Proceedings', 'author': 'Nelson S.', 'year': '1966'} / Head Injury Conference Proceedings by Nelson S. (1966)
10.1152/ajplegacy.1948.154.1.73 / Am. J. Physiol. by Richter D. (1948)
10.1152/ajplegacy.1954.179.1.85 / Am. J. Physiol. by Rosomoff H. L. (1954)
10.1042/bj0321908
10.1111/j.1471-4159.1971.tb05067.x
10.1111/j.1471-4159.1961.tb13509.x
10.1007/BF00362995
{'key': 'e_1_2_1_22_1', 'first-page': '251', 'volume': '101', 'author': 'Zlotnik I.', 'year': '1960', 'journal-title': 'Q. Jl Microsc. Sci.'} / Q. Jl Microsc. Sci. by Zlotnik I. (1960)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 4, 2006, 11:05 p.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 4:11 p.m.)
Indexed	1 day ago (Sept. 4, 2025, 9:44 a.m.)
Issued	53 years, 5 months ago (April 1, 1972)
Published	53 years, 5 months ago (April 1, 1972)
Published Online	18 years, 11 months ago (Oct. 4, 2006)
Published Print	53 years, 5 months ago (April 1, 1972)

Funders 0

None

BibTeX

@article{Ferrendelli_1972, title={QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREON‐121}, volume={19}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1972.tb01418.x}, DOI={10.1111/j.1471-4159.1972.tb01418.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Ferrendelli, J. A. and Gay, M. H. and Sedgwick, W. G. and Chang, M. M.}, year={1972}, month=apr, pages={979–987} }

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