DOI: 10.1111/j.1471-4159.1972.tb01326.x. DEMONSTRATION OF ACETYLCHOLINE RELEASE BY MEASURING EFFLUX OF LABELLED CHOLINE FROM CEREBRAL CORTICAL SLICES

DEMONSTRATION OF ACETYLCHOLINE RELEASE BY MEASURING EFFLUX OF LABELLED CHOLINE FROM CEREBRAL CORTICAL SLICES

10.1111/j.1471-4159.1972.tb01326.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract— To demonstrate release of ACh in the absence of inhibition of cholinesterase, slices of cerebral cortex were incubated with [3H]choline, after which they were placed in a tissue bath for superfusion. Hemicholinium (HC‐3) increased the spontaneous efflux of [3H]choline. Electrical stimulation at 4/s increased the efflux of [3H]choline to the same extent whether the slices were stimulated early or late during superfusion. The effect of stimulation on efflux of [3H]choline was abolished by tetrodotoxin and by the absence of calcium. The extent of choline efflux resulting from stimulation, as calculated from the specific radioactivity of the incubation medium, was the same when the slices were incubated with 0.1 or 1.0mM choline, but was less with lower concentrations of choline. We conclude that the increased efflux of [3H]choline evoked by stimulation probably originates from stores of [3H]ACh synthetized during incubation.

Bibliography

Somogyi, G. T., & Szerb, J. C. (1972). DEMONSTRATION OF ACETYLCHOLINE RELEASE BY MEASURING EFFLUX OF LABELLED CHOLINE FROM CEREBRAL CORTICAL SLICES. Journal of Neurochemistry, 19(11), 2667â2677. Portico.

Authors 2

G. T. Somogyi (first)
J. C. Szerb (additional)

References 27 Referenced 82

{'key': 'e_1_2_2_2_1', 'first-page': '31', 'volume': '156', 'author': 'Baldessarini R. J.', 'year': '1967', 'journal-title': 'J. Pharmac. exp. Ther.'} / J. Pharmac. exp. Ther. by Baldessarini R. J. (1967)
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{'key': 'e_1_2_2_6_1', 'first-page': '440p', 'volume': '42', 'author': 'Bourdois P. S.', 'year': '1971', 'journal-title': 'Br. J. Pharmac.'} / Br. J. Pharmac. by Bourdois P. S. (1971)
10.1016/0006-291X(71)90202-6
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{'key': 'e_1_2_2_9_1', 'first-page': '163', 'volume-title': 'Drugs and Cholinergic Mechanisms in rhe CNS', 'author': 'Collier B.', 'year': '1970'} / Drugs and Cholinergic Mechanisms in rhe CNS by Collier B. (1970)
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{'key': 'e_1_2_2_15_1', 'volume-title': 'The Uptake and Storage of Noradrenaline in Sympthetic Nerves', 'author': 'Iversen L. L.', 'year': '1967'} / The Uptake and Storage of Noradrenaline in Sympthetic Nerves by Iversen L. L. (1967)
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10.1111/j.1471-4159.1971.tb11999.x
10.1042/bj0570666 / Biochem. J. by Rowsell E. V. (1954)
10.1111/j.1471-4159.1966.tb09866.x
10.1111/j.1471-4159.1966.tb06809.x
10.1111/j.1471-4159.1969.tb05971.x

Dates

Type	When
Created	18 years, 11 months ago (Oct. 4, 2006, 10:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 8, 2023, 1:48 a.m.)
Indexed	1 day ago (Sept. 4, 2025, 10:03 a.m.)
Issued	52 years, 10 months ago (Nov. 1, 1972)
Published	52 years, 10 months ago (Nov. 1, 1972)
Published Online	18 years, 11 months ago (Oct. 4, 2006)
Published Print	52 years, 10 months ago (Nov. 1, 1972)

Funders 0

None

BibTeX

@article{Somogyi_1972, title={DEMONSTRATION OF ACETYLCHOLINE RELEASE BY MEASURING EFFLUX OF LABELLED CHOLINE FROM CEREBRAL CORTICAL SLICES}, volume={19}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1972.tb01326.x}, DOI={10.1111/j.1471-4159.1972.tb01326.x}, number={11}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Somogyi, G. T. and Szerb, J. C.}, year={1972}, month=nov, pages={2667–2677} }

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