DOI: 10.1111/j.1471-4159.1970.tb00495.x. TRIPHOSPHOINOSITIDE PHOSPHODIESTERASE IN DEVELOPING BRAIN OF THE RAT AND IN SUBCELLULAR FRACTIONS OF BRAIN

TRIPHOSPHOINOSITIDE PHOSPHODIESTERASE IN DEVELOPING BRAIN OF THE RAT AND IN SUBCELLULAR FRACTIONS OF BRAIN

10.1111/j.1471-4159.1970.tb00495.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract— An assay system for the measurement of triphosphoinositide phosphodiesterase in homogenates of rat brain is described. With triphosphoinositide (TPI) as substrate, and in the presence of 0·1 m‐KCI and saturating amounts of diethyl ether, the activity of phosphodiesterase in myelinated brain was 400–500 μmoles of TPI hydrolysed per g wet wt. per hr. One quarter of the adult level of the enzyme was present in rat brain one day after birth, with the remainder being added prior to and during the early stages of myelination. On subfractionation of brain homogenates, substantial activity of the enzyme was located in the soluble portion and in the paniculate fractions enriched in myelin and synaptosomes. The enzyme associated with the particulate fractions could not be detached from the membranes by any of several methods employed. There was a rough correlation between distribution of phosphodiesterase and that of 5′‐nucleotidase, an enzyme associated with plasma membrane in a number of tissues. Some implications of the results are discussed.

Bibliography

Keough, K. M. W., & Thompson, W. (1970). TRIPHOSPHOINOSITIDE PHOSPHODIESTERASE IN DEVELOPING BRAIN OF THE RAT AND IN SUBCELLULAR FRACTIONS OF BRAIN. Journal of Neurochemistry, 17(1), 1â11. Portico.

Authors 2

K. M. W Keough (first)
W Thompson (additional)

References 33 Referenced 43

10.1042/bj0730270
10.1016/S0021-9258(18)70226-3
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10.1016/0005-2744(69)90107-7
{'key': 'e_1_2_2_14_1', 'first-page': '155', 'volume': '331', 'author': 'Horhammer L.', 'year': '1959', 'journal-title': 'Biochem. Z.'} / Biochem. Z. by Horhammer L. (1959)
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{'key': 'e_1_2_2_21_1', 'first-page': '273', 'volume-title': 'Biochemistry and the Central Nervous System', 'author': 'Ilwain H.', 'year': '1966'} / Biochemistry and the Central Nervous System by Ilwain H. (1966)
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10.1139/o67-095
10.1042/bj0910233 / Biochem. J. by Thompson W. (1964)
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10.1021/bi00862a026

Dates

Type	When
Created	18 years, 10 months ago (Oct. 4, 2006, 10:20 p.m.)
Deposited	1 year, 9 months ago (Nov. 4, 2023, 6:33 a.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:26 a.m.)
Issued	55 years, 7 months ago (Jan. 1, 1970)
Published	55 years, 7 months ago (Jan. 1, 1970)
Published Online	18 years, 10 months ago (Oct. 4, 2006)
Published Print	55 years, 7 months ago (Jan. 1, 1970)

Funders 0

None

BibTeX

@article{Keough_1970, title={TRIPHOSPHOINOSITIDE PHOSPHODIESTERASE IN DEVELOPING BRAIN OF THE RAT AND IN SUBCELLULAR FRACTIONS OF BRAIN}, volume={17}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1970.tb00495.x}, DOI={10.1111/j.1471-4159.1970.tb00495.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Keough, K. M. W and Thompson, W}, year={1970}, month=jan, pages={1–11} }

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