DOI: 10.1111/j.1471-4159.1969.tb09904.x. METABOLISM OF GLUCOSE AND GLUTAMATE BY SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX

METABOLISM OF GLUCOSE AND GLUTAMATE BY SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX

10.1111/j.1471-4159.1969.tb09904.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract—(1) Synaptosomes incubated in high sodium, low potassium media showed high linear respiration in the presence of glucose which was converted into lactate, aspartate, glutamate, glutamine, alanine and GABA during 1 hr incubation periods.(2) Total conversion of glucose into most of these substrates over the incubation period was similar in synaptosomes and cortex slices. Half the lactate and only a small fraction of the glutamine made by slices was formed by synaptosomes.(3) Pool sizes of amino acids in cortex slices after incubation with glucose were, in general, higher than in synaptosomes, glutamate and glutamine being four‐fold higher in slices.(4) Most of the amino acids made from glucose by synaptosomes were contained within their structure and not lost to the medium.(5) Glutamate was actively metabolized by synaptosomes to aspartate, glutamine, alanine and GABA. The specific radioactivities of the amino acids (except glutamine) after 1 hr incubation, approached that of the glutamate.(6) Pyridoxal phosphate added to the incubation medium increased GABA production from glutamate but not from glucose.

Bibliography

Bradford, H. F., & Thomas, A. J. (1969). METABOLISM OF GLUCOSE AND GLUTAMATE BY SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX. Journal of Neurochemistry, 16(11), 1495â1504. Portico.

Authors 2

H. F. Bradford (first)
A. J. Thomas (additional)

References 24 Referenced 126

10.1016/0304-4165(66)90129-2
10.1111/j.1471-4159.1966.tb10285.x
10.1098/rspb.1955.0031
{'key': 'e_1_2_2_5_1', 'volume-title': 'Methods of Enzymatic Analysis', 'author': 'Bergmeyer A. U.', 'year': '1965'} / Methods of Enzymatic Analysis by Bergmeyer A. U. (1965)
10.1111/j.1471-4159.1966.tb04291.x
BradfordH. F.(1967)Abstr.1st Int. Neurochem. Congr. Strasbourg.p.30.
10.1111/j.1471-4159.1969.tb06444.x
10.1111/j.1471-4159.1969.tb08987.x
10.1111/j.1471-4159.1962.tb07489.x
{'key': 'e_1_2_2_11_1', 'first-page': '88', 'volume-title': 'Nerve as a Tissue', 'author': 'Robertis E.', 'year': '1967'} / Nerve as a Tissue by Robertis E. (1967)
10.1152/jn.1948.11.6.473 / J. Neurophysiol. by Elliott K. A. C. (1948)
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{'key': 'e_1_2_2_14_1', 'first-page': '79', 'volume': '96', 'author': 'Gray E. G.', 'year': '1962', 'journal-title': 'J. Anat. (Lond.)'} / J. Anat. (Lond.) by Gray E. G. (1962)
10.1042/bj0880404
10.1111/j.1471-4159.1968.tb10316.x
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{'key': 'e_1_2_2_19_1', 'volume-title': 'Practical Neurochemistry', 'author': 'McIlwain H.', 'year': '1962'} / Practical Neurochemistry by McIlwain H. (1962)
10.1111/j.1471-4159.1964.tb06124.x
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10.1098/rspb.1962.0035
10.1111/j.1471-4159.1965.tb06751.x
10.1042/bj0990005C / Biochem. J. by Thomas A. J. (1966)
{'key': 'e_1_2_2_25_1', 'volume-title': 'Automation, Mechanisation and Data Handling in Microbiology', 'author': 'Thomas A. J.', 'year': '1969'} / Automation, Mechanisation and Data Handling in Microbiology by Thomas A. J. (1969)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 4, 2006, 9:58 p.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 9:14 a.m.)
Indexed	1 day, 17 hours ago (Sept. 4, 2025, 10:17 a.m.)
Issued	55 years, 10 months ago (Nov. 1, 1969)
Published	55 years, 10 months ago (Nov. 1, 1969)
Published Online	18 years, 11 months ago (Oct. 4, 2006)
Published Print	55 years, 10 months ago (Nov. 1, 1969)

Funders 0

None

BibTeX

@article{Bradford_1969, title={METABOLISM OF GLUCOSE AND GLUTAMATE BY SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX}, volume={16}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1969.tb09904.x}, DOI={10.1111/j.1471-4159.1969.tb09904.x}, number={11}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Bradford, H. F. and Thomas, A. J.}, year={1969}, month=nov, pages={1495–1504} }

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