DOI: 10.1111/j.1470-8744.1989.tb00068.x. Purification of Two Muscle Enzymes by Chromatography on Immobilized Ferric Ions

Purification of Two Muscle Enzymes by Chromatography on Immobilized Ferric Ions

10.1111/j.1470-8744.1989.tb00068.x

Crossref journal-article

Wiley

Biotechnology and Applied Biochemistry (311)

Abstract

Two enzymes, glycogen phosphorylase and lactate dehydrogenase, were purified simultaneously in a single step. Ferric ions immobilized on a chelating gel were used as the adsorbent. Adsorption and desorption steps were accomplished by changes in buffer composition. The recoveries were better than 80% and the capacities were about 5 mg of protein per milliliter of adsorbent. The procedure worked well both on a small and on a preparative scale. The homogeneity of the purified enzymes was checked by FPLC.

Bibliography

Chaga, G., Andersson, L., Ersson, B., & Porath, J. (1989). Purification of Two Muscle Enzymes by Chromatography on Immobilized Ferric Ions. Biotechnology and Applied Biochemistry, 11(4), 424â431. Portico.

Authors 4

G. Chaga (first)
L. Andersson (additional)
B. Ersson (additional)
J. Porath (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	1 year, 10 months ago (Oct. 20, 2023, 10:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 10:42 p.m.)
Indexed	1 year, 5 months ago (March 24, 2024, 6:19 a.m.)
Issued	36 years ago (Aug. 1, 1989)
Published	36 years ago (Aug. 1, 1989)
Published Online	14 years, 8 months ago (Dec. 23, 2010)
Published Print	36 years ago (Aug. 1, 1989)

Funders 0

None

BibTeX

@article{Chaga_1989, title={Purification of Two Muscle Enzymes by Chromatography on Immobilized Ferric Ions}, volume={11}, ISSN={1470-8744}, url={http://dx.doi.org/10.1111/j.1470-8744.1989.tb00068.x}, DOI={10.1111/j.1470-8744.1989.tb00068.x}, number={4}, journal={Biotechnology and Applied Biochemistry}, publisher={Wiley}, author={Chaga, G. and Andersson, L. and Ersson, B. and Porath, J.}, year={1989}, month=aug, pages={424–431} }

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