DOI: 10.1111/j.1469-8137.1994.tb02954.x. Extra‐ and intra‐cellular carbonic anhydrase in relation to culture age in a high‐calcifying strain of <i>Emiliania huxleyi</i> Lohmann

Extra‐ and intra‐cellular carbonic anhydrase in relation to culture age in a high‐calcifying strain of Emiliania huxleyi Lohmann

10.1111/j.1469-8137.1994.tb02954.x

Crossref journal-article

Wiley

New Phytologist (311)

Abstract

SUMMARYThe relationships between extra‐ and intra‐cellular carbonic anhydrase, calcification rate, utilization of dissolved inorganic carbon (DIG) and culture age were investigated in a high‐calcifying strain of Emiliania huxleyi. The detection of carbonic anhydrasc was dependent on culture age; neither the extra‐cellular activity of intact cells nor activity in crude homogenates was detected until the stationary phase. By the stationary phase DIC in the medium was totally depleted and the calcification rate had decreased by 60°0. Extra‐ and intra‐cellular carbonic anhydrase was stimulated by Na+but not Cl ions. The isolation of intact organelles by isopycnic gradient centrifugal ion from exponential and stationary phase cultures of high calcifying cells showed high carbonic anhydrase activity in the chloroplast fraction but carbonic anhydrase was not detected in low‐calcifying cells even after gradient centrifugation. Although 50/IM ethoxyzolamide was an effective inhibitor of carbonic anhydrase activity in vitro, photosynthetic 14CO2 fixation was only inhibited 30%, calcification rate 10%, and the internal inorganic carbon pool unaffected in intact cells. It is proposed that chloroplast carbonic anhydrase maintains the steady state flux of COj, from the chloroplast envelope to Rubisco, much of the CO2 arising by release from HCO3−in the cytosol, required to maintain cytoplasmic pH near neutrality in high‐calcifying cells.

Bibliography

NIMER, N. A., GUAN, Q., & MERRETT, M. J. (1994). Extraâ and intraâcellular carbonic anhydrase in relation to culture age in a highâcalcifying strain of Emiliania huxleyi Lohmann. New Phytologist, 126(4), 601â607. Portico.

Authors 3

N. A. NIMER (first)
Q. GUAN (additional)
M. J. MERRETT (additional)

References 38 Referenced 50

10.1016/0014-5793(84)81013-3
10.1104/pp.66.3.407
10.1016/0003-2697(76)90527-3
BrownleeC NimerN DongLF MerrettMJ.1993Cellular regulation during calcification in Emiliania huxleyi.In:GreenJ.LeadbeaterB eds.Biology of the Prymnesiophyres Systemafics Society (in the press).
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10.1111/j.1469-8137.1972.tb04068.x
10.1111/j.0022-3646.1992.00619.x
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10.1111/j.1469-8137.1993.tb03777.x
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{'key': 'e_1_2_1_16_1', 'first-page': '4S3', 'article-title': 'Extinction coefficients of chlorophyll a and b in N, N‐dimethylformamide and 80%, acetone', 'volume': '77', 'author': 'Inskecp WP', 'year': '1985', 'journal-title': 'Plant Physiology'} / Plant Physiology / Extinction coefficients of chlorophyll a and b in N, N‐dimethylformamide and 80%, acetone by Inskecp WP (1985)
10.1093/pcp/24.2.255
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10.1111/j.1399-3054.1963.tb08302.x
{'key': 'e_1_2_1_28_1', 'first-page': '1', 'article-title': 'A tracer study of the inorganic carbon uptake during coccolith formation and photosynthesis in the occolithophorid Coccolithus huxleyi', 'volume': '3', 'author': 'Paasche E.', 'year': '1964', 'journal-title': 'Physiohgia Plantarum Supplementum'} / Physiohgia Plantarum Supplementum / A tracer study of the inorganic carbon uptake during coccolith formation and photosynthesis in the occolithophorid Coccolithus huxleyi by Paasche E. (1964)
10.1007/BF01369778
10.1111/j.0022-3646.1993.00321.x
10.4319/lo.1980.25.2.0248
10.1111/j.1529-8817.1982.tb03205.x
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10.1007/BF00387911
10.1016/S0044-328X(83)80212-8
10.1016/0304-3770(89)90051-X
10.1104/pp.70.2.340
{'key': 'e_1_2_1_39_1', 'first-page': '1509', 'article-title': 'Carbonic anhydrase of a unicellular red alga Porphyridium cruentum R‐l, 11 Distribution and role in photosynthesis', 'volume': '28', 'author': 'Yagawa Y', 'year': '1987', 'journal-title': 'Plant Cell Physiology'} / Plant Cell Physiology / Carbonic anhydrase of a unicellular red alga Porphyridium cruentum R‐l, 11 Distribution and role in photosynthesis by Yagawa Y (1987)

Dates

Type	When
Created	19 years, 3 months ago (April 29, 2006, 7:21 a.m.)
Deposited	1 year, 11 months ago (Sept. 1, 2023, 3:21 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 4:23 p.m.)
Issued	31 years, 4 months ago (April 1, 1994)
Published	31 years, 4 months ago (April 1, 1994)
Published Online	19 years, 3 months ago (April 28, 2006)
Published Print	31 years, 4 months ago (April 1, 1994)

Funders 0

None

BibTeX

@article{NIMER_1994, title={Extra‐ and intra‐cellular carbonic anhydrase in relation to culture age in a high‐calcifying strain of Emiliania huxleyi Lohmann}, volume={126}, ISSN={1469-8137}, url={http://dx.doi.org/10.1111/j.1469-8137.1994.tb02954.x}, DOI={10.1111/j.1469-8137.1994.tb02954.x}, number={4}, journal={New Phytologist}, publisher={Wiley}, author={NIMER, N. A. and GUAN, Q. and MERRETT, M. J.}, year={1994}, month=apr, pages={601–607} }

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