DOI: 10.1111/j.1469-7793.2000.t01-2-00083.x. Calcium‐, voltage‐ and osmotic stress‐sensitive currents in <i>Xenopus</i> oocytes and their relationship to single mechanically gated channels

Calcium‐, voltage‐ and osmotic stress‐sensitive currents in Xenopus oocytes and their relationship to single mechanically gated channels

10.1111/j.1469-7793.2000.t01-2-00083.x

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

Patch recordings from Xenopus oocytes indicated that mechanically gated (MG) channels are expressed at a uniform surface density (∼1 channel μm−2) with an estimated > 3 × 106 MG channels per oocyte that could generate microamps of current at ±50 mV. Removal of external Ca2+ induced a membrane conductance that differed from MG channels in ion selectivity, pharmacology and sensitivity to connexion‐38. Depolarization to +50 mV activated a Na+‐selective, a Cl−‐selective and a non‐selective conductance. Hyperpolarization to −150 mV activated a non‐selective conductance. None of these conductances appeared to be mediated by MG channels. Hypotonicity (25 %) failed to evoke any change in membrane conductance in the majority of defolliculated oocytes. Hypertonicity (200 %) evoked a large non‐selective (PK/PCl≈ 1) membrane conductance that was not blocked by 100 μM Gd3+. Although the above stimuli could activate a variety of whole‐oocyte conductances, including three novel conductances, they did not involve MG channel activation. Possible mechanisms underlying the discrepancy between observed conductances and those anticipated from patch‐clamp studies are discussed.

Bibliography

Zhang, Y., & Hamill, O. P. (2000). Calciumâ, voltageâ and osmotic stressâsensitive currents in Xenopus oocytes and their relationship to single mechanically gated channels. The Journal of Physiology, 523(1), 83â99. Portico.

Authors 2

Yong Zhang (first)
Owen P. Hamill (additional)

References 50 Referenced 29

10.1152/ajpcell.1996.270.1.C224
10.1085/jgp.103.2.153
10.1113/jphysiol.1995.sp020971
10.1113/jphysiol.1995.sp020689
10.1113/jphysiol.1983.sp014852
10.1098/rspb.1982.0040
10.1073/pnas.79.10.3188
{'key': 'e_1_2_5_8_1', 'first-page': '85', 'article-title': 'Freeze‐fracture electron microscopy of membrane changes in progesterone‐induced maturing oocytes and eggs of Xenopus laevis', 'volume': '31', 'author': 'Bluemink J. G.', 'year': '1983', 'journal-title': 'European Journal of Cell Biology'} / European Journal of Cell Biology / Freeze‐fracture electron microscopy of membrane changes in progesterone‐induced maturing oocytes and eggs of Xenopus laevis by Bluemink J. G. (1983)
10.1016/S0006-3495(96)79273-1
10.1016/S0006-3495(99)77463-1
10.1111/j.1469-7793.1997.569bj.x
10.1007/978-1-4615-7858-1_24
10.1073/pnas.89.16.7462
10.1385/0-89603-311-2:75
{'key': 'e_1_2_5_15_1', 'first-page': '231', 'article-title': 'The pharmacology of mechanogated membrane ion channels', 'volume': '48', 'author': 'Hamill O. P.', 'year': '1996', 'journal-title': 'Pharmacological Reviews'} / Pharmacological Reviews / The pharmacology of mechanogated membrane ion channels by Hamill O. P. (1996)
{'key': 'e_1_2_5_16_1', 'first-page': 'A348', 'article-title': 'Membrane voltage and tension interactions in the gating of the mechano‐gated cation channel in Xenopus oocytes', 'volume': '70', 'author': 'Hamill O. P.', 'year': '1996', 'journal-title': 'Biophysical Journal'} / Biophysical Journal / Membrane voltage and tension interactions in the gating of the mechano‐gated cation channel in Xenopus oocytes by Hamill O. P. (1996)
10.1146/annurev.physiol.59.1.621
10.1007/BF00656997
{'key': 'e_1_2_5_19_1', 'volume-title': 'Ionic Channels of Excitable Membranes', 'author': 'Hille B.', 'year': '1992'} / Ionic Channels of Excitable Membranes by Hille B. (1992)
10.1085/jgp.103.2.217
10.1113/jphysiol.1991.sp018755
10.1111/j.1476-5381.1992.tb14329.x
10.1111/j.1476-5381.1993.tb13449.x
10.1007/BF00375058
10.1007/BF00582635
10.1126/science.1706535
10.1098/rspb.1990.0005
10.1098/rspb.1988.0018
10.1016/S0005-2736(98)00257-0
10.1146/annurev.ph.57.030195.002001
{'key': 'e_1_2_5_31_1', 'first-page': '31', 'volume-title': 'Single‐channel Recording', 'author': 'Sakmann B.', 'year': '1983'} / Single‐channel Recording by Sakmann B. (1983)
{'key': 'e_1_2_5_32_1', 'first-page': '368', 'article-title': 'A new technique for evaluating volume sensitivity of ion channels', 'volume': '433', 'author': 'Schutt W.', 'year': '1997', 'journal-title': 'Pflügers Archiv'} / Pflügers Archiv / A new technique for evaluating volume sensitivity of ion channels by Schutt W. (1997)
10.1111/j.1469-7793.1997.551ba.x
{'key': 'e_1_2_5_34_1', 'first-page': '139', 'article-title': 'Mechanosensitive ion channels: Single channel vs. whole cell activities', 'volume': '6', 'author': 'Sokabe M.', 'year': '1997', 'journal-title': 'Progress in Cell Research'} / Progress in Cell Research / Mechanosensitive ion channels: Single channel vs. whole cell activities by Sokabe M. (1997)
10.1083/jcb.111.2.599
10.1111/j.1440-169X.1991.00437.x
10.1007/978-1-4419-1229-9_15
10.1113/jphysiol.1988.sp017417
10.1113/jphysiol.1996.sp021718
10.1073/pnas.93.12.5836
10.1073/pnas.92.18.8478
10.1007/s002329900430
10.1007/s004240050554
10.1126/science.2466333
10.1113/jphysiol.1990.sp018322
10.1007/BF00234157
Zhang Y.(1998).Membrane patch and whole cell response ofXenopusoocytes to mechanical electrical and osmotic stimulation.PhD Dissertation University of Texas Medical Branch TX USA.
10.1111/j.1469-7793.2000.t01-1-00117.x
10.1111/j.1469-7793.2000.00101.x
10.1111/j.1469-7793.1998.763bp.x

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 1:06 p.m.)
Deposited	1 year, 11 months ago (Sept. 4, 2023, 5:18 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 3:58 p.m.)
Issued	25 years, 7 months ago (Feb. 1, 2000)
Published	25 years, 7 months ago (Feb. 1, 2000)
Published Online	25 years, 7 months ago (Feb. 1, 2000)
Published Print	25 years, 7 months ago (Feb. 1, 2000)

Funders 0

None

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