DOI: 10.1111/j.1469-7793.2000.00101.x. On the discrepancy between whole‐cell and membrane patch mechanosensitivity in <i>Xenopus</i> oocytes

On the discrepancy between whole‐cell and membrane patch mechanosensitivity in Xenopus oocytes

10.1111/j.1469-7793.2000.00101.x

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

Mechanical stimulation of voltage‐clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension‐sensitive recruitment of new membrane. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can ‘buffer’ membrane tension changes and thus prevent MG channel activation. High‐resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.

Bibliography

Zhang, Y., & Hamill, O. P. (2000). On the discrepancy between wholeâcell and membrane patch mechanosensitivity in Xenopus oocytes. The Journal of Physiology, 523(1), 101â115. Portico.

Authors 2

Yong Zhang (first)
Owen P. Hamill (additional)

References 50 Referenced 60

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Dates

Type	When
Created	21 years ago (Aug. 13, 2004, 3:40 a.m.)
Deposited	1 year, 11 months ago (Sept. 4, 2023, 5:18 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 3:52 p.m.)
Issued	25 years, 7 months ago (Feb. 1, 2000)
Published	25 years, 7 months ago (Feb. 1, 2000)
Published Online	25 years, 7 months ago (Feb. 1, 2000)
Published Print	25 years, 7 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Zhang_2000, title={On the discrepancy between whole‐cell and membrane patch mechanosensitivity in Xenopus oocytes}, volume={523}, ISSN={1469-7793}, url={http://dx.doi.org/10.1111/j.1469-7793.2000.00101.x}, DOI={10.1111/j.1469-7793.2000.00101.x}, number={1}, journal={The Journal of Physiology}, publisher={Wiley}, author={Zhang, Yong and Hamill, Owen P.}, year={2000}, month=feb, pages={101–115} }

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