DOI: 10.1111/j.1469-7793.1997.605ba.x. Calcium action potentials restricted to distal apical dendrites of rat neocortical pyramidal neurons

Calcium action potentials restricted to distal apical dendrites of rat neocortical pyramidal neurons

10.1111/j.1469-7793.1997.605ba.x

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

Simultaneous whole‐cell voltage and Ca2+ fluorescence measurements were made from the distal apical dendrites and the soma of thick tufted pyramidal neurons in layer 5 of 4‐week‐old (P28–32) rat neocortex slices to investigate whether activation of distal synaptic inputs can initiate regenerative responses in dendrites. Dual whole‐cell voltage recordings from the distal apical trunk and primary tuft branches (540–940 μm distal to the soma) showed that distal synaptic stimulation (upper layer 2) evoking a subthreshold depolarization at the soma could initiate regenerative potentials in distal branches of the apical tuft which were either graded or all‐or‐none. These regenerative potentials did not propagate actively to the soma and axon. Calcium fluorescence measurements along the apical dendrites indicated that the regenerative potentials were associated with a transient increase in the concentration of intracellular free calcium ([Ca2+]i) restricted to distal dendrites. Cadmium added to the bath solution blocked both the all‐or‐none dendritic regenerative potentials and local dendritic [Ca2+]i transients evoked by distal dendritic current injection. Thus, the regenerative potentials in distal dendrites represent local Ca2+ action potentials. Initiation of distal Ca2+ action potentials by a synaptic stimulus required coactivation of AMPA‐ and NMDA‐type glutamate receptor channels. It is concluded that in neocortical layer 5 pyramidal neurons of P28–32 animals glutamatergic synaptic inputs to the distal apical dendrites can be amplified via local Ca2+ action potentials which do not reach threshold for axonal AP initiation. As amplification of distal excitatory synaptic input is associated with a localized increase in [Ca2+]i these Ca2+ action potentials could control the synaptic efficacy of the distal cortico‐cortical inputs to layer 5 pyramidal neurons.

Bibliography

Schiller, J., Schiller, Y., Stuart, G., & Sakmann, B. (1997). Calcium action potentials restricted to distal apical dendrites of rat neocortical pyramidal neurons. The Journal of Physiology, 505(3), 605â616. Portico.

Authors 4

Jackie Schiller (first)
Yitzhak Schiller (additional)
Greg Stuart (additional)
Bert Sakmann (additional)

References 43 Referenced 423

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Dates

Type	When
Created	20 years, 11 months ago (Sept. 30, 2004, 2:54 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 12:39 a.m.)
Indexed	3 months ago (June 7, 2025, 12:15 a.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Online	20 years, 11 months ago (Sept. 29, 2004)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Schiller_1997, title={Calcium action potentials restricted to distal apical dendrites of rat neocortical pyramidal neurons}, volume={505}, ISSN={1469-7793}, url={http://dx.doi.org/10.1111/j.1469-7793.1997.605ba.x}, DOI={10.1111/j.1469-7793.1997.605ba.x}, number={3}, journal={The Journal of Physiology}, publisher={Wiley}, author={Schiller, Jackie and Schiller, Yitzhak and Stuart, Greg and Sakmann, Bert}, year={1997}, month=dec, pages={605–616} }

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