DOI: 10.1111/j.1460-9568.1997.tb01362.x. Locatization of Voltaae‐sensitive Ca<sup>2+</sup> Fluxes and Neuropeptide Y Immunoreactivity to Varicosities in SH‐SY5Y Human Neuroblastoma Cells Differentiated by Treatment with the Protein Kinase Inhibitor Staurosporine

Locatization of Voltaae‐sensitive Ca2+ Fluxes and Neuropeptide Y Immunoreactivity to Varicosities in SH‐SY5Y Human Neuroblastoma Cells Differentiated by Treatment with the Protein Kinase Inhibitor Staurosporine

10.1111/j.1460-9568.1997.tb01362.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractThe distribution of voltage‐sensitive elevations of the level of Ca2+ in untreated SH‐SY5Y cells and cells that had been induced to differentiate with staurosporine was investigated by monitoring fura‐2 fluorescence in cell suspensions, and by using microfluorometry and quantitative fluorescence imaging on cell bodies and on cellular processes. Cell bodies of both types of cells displayed small Ca2+ elevations, which were composed of transient and sustained components. Elevations were partially sensitive to the L‐ and N‐channel blockers nifedipine (1 μM) and ω‐conotoxin GVIA (100 nM) respectively. Up to ten times higher Ca2+ elevations were observed in varicosities of treated cells than in cell bodies of treated and untreated cells. These elevations were insensitive to compounds known to release Ca2+ from intracellular stores. Elevations of Ca2+ were sustained, and they were insensitive to 5 pM nifedipine, 100 nM ω‐agatoxin IVA and 100 nM ω‐conotoxin GVIA, and partially sensitive to 2 pM ω‐conotoxin GVIA, indicating predominance of non‐L‐type, non‐N‐type, non‐P‐type channel activity. The intracellular localization of neuropeptide Y, a marker of differentiation in these cells, was also investigated by fluorescence immunocytochemistry. Varicosities of treated cells displayed marked fluorescence when viewed in a confocal microscope. These findings show that the varicosities of staurosporine‐treated cells exhibit some of the functional properties of nerve terminals. The varicosities resemble boutons en passant nerve endings and they seem to express Ca2+ channels different from those in the cell body.

Bibliography

Kukkonen, J. P., Shariatmadari, R., Courtney, M. J., & Akerman, K. E. O. (1997). Locatization of Voltaaeâsensitive Ca2+ Fluxes and Neuropeptide Y Immunoreactivity to Varicosities in SHâSY5Y Human Neuroblastoma Cells Differentiated by Treatment with the Protein Kinase Inhibitor Staurosporine. European Journal of Neuroscience, 9(1), 140â150. Portico.

Authors 4

Jyrki P. Kukkonen (first)
Ramin Shariatmadari (additional)
Michael J. Courtney (additional)
Karl E. O. Akerman (additional)

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Dates

Type	When
Created	19 years, 4 months ago (April 7, 2006, 10:26 p.m.)
Deposited	1 year, 10 months ago (Oct. 3, 2023, 7:04 a.m.)
Indexed	1 year, 6 months ago (Feb. 20, 2024, 12:39 p.m.)
Issued	28 years, 7 months ago (Jan. 1, 1997)
Published	28 years, 7 months ago (Jan. 1, 1997)
Published Online	19 years, 4 months ago (April 7, 2006)
Published Print	28 years, 7 months ago (Jan. 1, 1997)

Funders 0

None

BibTeX

@article{Kukkonen_1997, title={Locatization of Voltaae‐sensitive Ca2+ Fluxes and Neuropeptide Y Immunoreactivity to Varicosities in SH‐SY5Y Human Neuroblastoma Cells Differentiated by Treatment with the Protein Kinase Inhibitor Staurosporine}, volume={9}, ISSN={1460-9568}, url={http://dx.doi.org/10.1111/j.1460-9568.1997.tb01362.x}, DOI={10.1111/j.1460-9568.1997.tb01362.x}, number={1}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Kukkonen, Jyrki P. and Shariatmadari, Ramin and Courtney, Michael J. and Akerman, Karl E. O.}, year={1997}, month=jan, pages={140–150} }

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