DOI: 10.1111/j.1460-9568.1995.tb00703.x. Immunohistochemical Visualization of Brain‐derived Neurotrophic Factor in the Rat Brain

Immunohistochemical Visualization of Brain‐derived Neurotrophic Factor in the Rat Brain

10.1111/j.1460-9568.1995.tb00703.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractA purified polyclonal antibody preparation was made against recombinant human brain‐derived neurotrophic factor (BDNF) in guinea pig and characterized for use in immunoassays and immunohistochemistry. The anti‐BDNF antibodies specifically recognized BDNF in Western blots and immunoprecipitation. There was no cross‐reactivity with the other known mammalian members of the neurotrophin family, nerve growth factor, neurotrophin‐3 and neurotrophin‐4/5. In immunohistochemical analysis, the anti‐BDNF recognized exogenous BDNF injected into the brain of rats, whereas no signal was obtained with the other neurotrophins. Preabsorption with native BDNF abolished the immunoreactivity in brain sections. These studies identify the anti‐BDNF as a tool for immunocytochemistry and the development of an immunoassay. Immunohistochemical analysis revealed widespread neuronal localization of BDNF in many brain areas. BDNF was localized in all subpopulations of hippocampal neurons. The distribution in the hippocampus suggests localization in the cytoplasm of cell bodies and dendrites.

Bibliography

DugichâDjordjevic, M. M., Peterson, C., Isono, F., Ohsawa, F., Widmer, H. R., Denton, T. L., Bennett, G. L., & Hefti, F. (1995). Immunohistochemical Visualization of Brainâderived Neurotrophic Factor in the Rat Brain. European Journal of Neuroscience, 7(9), 1831â1839. Portico.

Authors 8

Millicent M. Dugich‐Djordjevic (first)
Christine Peterson (additional)
Fujio Isono (additional)
Fukuichi Ohsawa (additional)
Hans R. Widmer (additional)
Timothy L. Denton (additional)
Gregory L. Bennett (additional)
Franz Hefti (additional)

References 29 Referenced 81

10.1016/0896-6273(89)90040-8
10.1073/pnas.88.22.10352
10.1016/0896-6273(92)90023-7
10.1002/cne.903190310
10.1002/jnr.490320402
10.1073/pnas.87.14.5454
10.1016/0896-6273(90)90090-3
10.1038/368147a0
{'key': 'e_1_2_2_10_1', 'first-page': '449', 'article-title': 'The extracellular domain of HER2/neu is a potential immunogen for active specific immunotherapy of breast cancer', 'volume': '9', 'author': 'Fendly B. M.', 'year': '1990', 'journal-title': 'J. Biol. Resp. Mod.'} / J. Biol. Resp. Mod. / The extracellular domain of HER2/neu is a potential immunogen for active specific immunotherapy of breast cancer by Fendly B. M. (1990)
10.1016/0169-328X(91)90136-L
10.1002/j.1460-2075.1990.tb07423.x
10.1038/344339a0
10.1016/0092-8674(94)90377-8
10.1126/science.2321006
10.1016/0896-6273(90)90089-X
10.1523/JNEUROSCI.13-07-02853.1993
10.1007/BF00966785
10.1126/science.283.5400.401
10.1126/science.1688328
10.1146/annurev.bi.62.070193.004135
10.1210/endo-129-3-1289
10.1179/014788889794651870 / J. Histotech. / A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radiolabeled single‐stranded RNA probes by Simmons D. M. (1989)
10.1002/cne.902800203
10.1016/0092-8674(94)90048-5
10.1111/j.1460-9568.1993.tb00526.x
10.1016/0014-4886(90)90068-4
10.1177/41.4.8450192
10.1523/JNEUROSCI.14-03-01688.1994 / J. Neurosci. / Regulation of brain‐derived neurotrophic factor (BDNF) expression and release from hippocampal neurons is mediated by non‐NMDA type glutamate receptors by Wetmore C. (1994)
10.1016/0378-5955(93)90202-C

Dates

Type	When
Created	19 years, 4 months ago (April 7, 2006, 9:55 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 4:50 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 11, 2024, 5:50 a.m.)
Issued	30 years ago (Sept. 1, 1995)
Published	30 years ago (Sept. 1, 1995)
Published Online	19 years, 4 months ago (April 7, 2006)
Published Print	30 years ago (Sept. 1, 1995)

Funders 0

None

BibTeX

@article{Dugich_Djordjevic_1995, title={Immunohistochemical Visualization of Brain‐derived Neurotrophic Factor in the Rat Brain}, volume={7}, ISSN={1460-9568}, url={http://dx.doi.org/10.1111/j.1460-9568.1995.tb00703.x}, DOI={10.1111/j.1460-9568.1995.tb00703.x}, number={9}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Dugich‐Djordjevic, Millicent M. and Peterson, Christine and Isono, Fujio and Ohsawa, Fukuichi and Widmer, Hans R. and Denton, Timothy L. and Bennett, Gregory L. and Hefti, Franz}, year={1995}, month=sep, pages={1831–1839} }

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