DOI: 10.1111/j.1460-9568.1995.tb00635.x. Epidermal Growth Factor Prevents Oxygen‐triggered Apoptosis and Induces Sustained Signalling in Cultured Rat Cerebral Cortical Neurons

Epidermal Growth Factor Prevents Oxygen‐triggered Apoptosis and Induces Sustained Signalling in Cultured Rat Cerebral Cortical Neurons

10.1111/j.1460-9568.1995.tb00635.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractEpidermal growth factor (EGF), a conventional mitogenic factor, acts as a neurotrophic factor on several types of neurons in the central nervous system. We found that EGF prevented the death of rat cerebral cortical neurons cultured in a 50% oxygen atmosphere. This high‐oxygen‐triggered cell death showed features of apoptotic cell death, which was blocked by inhibitors of RNA or protein synthesis. EGF prevented the oxygen‐induced death of the cultured cortical neurons in a dose‐dependent manner. Basic fibroblast growth factor (bFGF) also prevented this cell death, although there was no apparent additive effect of EGF and bFGF. Among the cultured cortical neurons, we observed neurons possessing the EGF receptor and cells expressing c‐Fos protein in response to EGF. The cortical neurons were cultured in the presence of cytosine arabinoside, and the number of glial fibrillary acidic protein‐positive astroglial cells was >0.5% of that of the corresponding microtubule‐associated protein 2‐positive neurons. Therefore, the effect of EGF on the cultured cortical neurons is thought to be due to a direct action. We also examined EGF‐induced signalling in the cultured cortical neurons. We found that EGF induced the sustained tyrosine phosphorylation of the EGF receptor and sustained the activation of mitogen‐activated protein kinase in the cultured cortical neurons. We suggest that EGF may exert the survival effect through the prolonged activation of the EGF signalling.

Bibliography

Yamada, M., Enokido, Y., Ikeuchi, T., & Hatanaka, H. (1995). Epidermal Growth Factor Prevents Oxygenâtriggered Apoptosis and Induces Sustained Signalling in Cultured Rat Cerebral Cortical Neurons. European Journal of Neuroscience, 7(10), 2130â2138. Portico.

Authors 4

Masashi Yamada (first)
Yasushi Enokido (additional)
Toshihiko Ikeuchi (additional)
Hiroshi Hatanaka (additional)

References 53 Referenced 47

10.1016/0006-8993(92)91433-F
10.1016/0012-1606(84)90007-1
10.1073/pnas.90.17.7915
10.1016/0896-6273(89)90040-8
10.1016/0092-8674(93)90508-N
10.1016/0304-3940(88)90132-2
10.1146/annurev.bi.56.070187.004313
10.1146/annurev.bi.48.070179.001205
10.1007/BF00174615
10.1126/science.6474175
10.1016/S0092-8674(88)80001-1
10.1016/0006-291X(91)92101-O
10.1016/S0021-9258(19)37174-1 / J. Biol. Chem. / Amyloidogenicity of A4‐ and A4‐bearing amyloid protein precursor fragments by metal‐catalyzed oxidation by Dyrks T. (1992)
10.1146/annurev.cb.07.110191.003311
10.1016/0006-8993(90)90004-U
10.1016/0306-4522(93)90041-D
10.1016/0006-8993(92)90400-4
10.1007/978-1-4757-9145-7_48
10.1016/0006-8993(88)91369-8
10.1111/j.1432-1033.1990.tb19384.x
10.1073/pnas.73.7.2424 / Proc. Natl Acad. Sci. USA / Establishment of noradrenergic clonal cell line of rat adrenal pheochromocytoma cells which respond to nerve growth factor by Greene L. A. (1976)
10.1016/0896-6273(95)90287-2
10.1016/0022-1759(89)90397-9
10.1046/j.1471-4159.1994.63051987.x
10.1016/0006-8993(81)91029-5
10.1016/0092-8674(93)80066-N
10.1083/jcb.88.1.189
10.1002/jnr.490250308
10.1016/0006-8993(90)91608-J
10.1016/0006-8993(90)90003-T
10.1523/JNEUROSCI.12-05-01688.1992 / J. Neurosci. / Regional distribution and developmental expression of epidermal growth factor and transforming growth factor‐α mRNA in mouse brain by a quantitative nuclease protection assay by Lazar L. M. (1992)
10.1111/j.1462-5822.2007.00901.x
10.1073/pnas.90.17.7951
10.1016/0092-8674(95)90401-8
10.1083/jcb.106.3.829
10.1073/pnas.83.19.7537
10.1126/science.3498986
10.1002/jnr.490210111
10.1016/0022-1759(83)90303-4
10.1038/312716a0
10.1016/0092-8674(93)90509-O
10.1016/0012-1606(90)90180-Q
10.1146/annurev.ne.14.030191.002321
10.1523/JNEUROSCI.12-05-01658.1992 / J. Neurosci. / Transgenic mice with increased Cu/Zn‐superoxide dismutase activity are resistant to N‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine‐induced neurotoxicity by Przedborski S. (1992)
10.1046/j.1471-4159.1994.62010376.x
10.1038/362059a0
10.1016/0968-0004(93)90031-H
10.1016/0896-6273(92)90177-F
10.1111/j.1471-4159.1992.tb08880.x
10.1016/0968-0004(94)90134-1
10.1111/j.1471-4159.1993.tb13631.x
10.1016/0165-3806(88)90009-0
10.1002/path.1711420112

Dates

Type	When
Created	19 years, 4 months ago (April 7, 2006, 9:34 p.m.)
Deposited	1 year, 11 months ago (Oct. 1, 2023, 5:46 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:39 p.m.)
Issued	29 years, 11 months ago (Oct. 1, 1995)
Published	29 years, 11 months ago (Oct. 1, 1995)
Published Online	19 years, 4 months ago (April 7, 2006)
Published Print	29 years, 11 months ago (Oct. 1, 1995)

Funders 0

None

BibTeX

@article{Yamada_1995, title={Epidermal Growth Factor Prevents Oxygen‐triggered Apoptosis and Induces Sustained Signalling in Cultured Rat Cerebral Cortical Neurons}, volume={7}, ISSN={1460-9568}, url={http://dx.doi.org/10.1111/j.1460-9568.1995.tb00635.x}, DOI={10.1111/j.1460-9568.1995.tb00635.x}, number={10}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Yamada, Masashi and Enokido, Yasushi and Ikeuchi, Toshihiko and Hatanaka, Hiroshi}, year={1995}, month=oct, pages={2130–2138} }

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