DOI: 10.1111/j.1460-9568.1994.tb00559.x. Neurotrophin‐4/5 Promotes Survival and Differentiation of Rat Striatal Neurons Developing in Culture

Neurotrophin‐4/5 Promotes Survival and Differentiation of Rat Striatal Neurons Developing in Culture

10.1111/j.1460-9568.1994.tb00559.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractCultures of dissociated striatal neurons from fetal rats were prepared, and were grown in the presence of neurotrophin‐4/5 (NT‐4/5) as well as the other known neurotrophins, nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF) and neurotrophin‐3 (NT‐3). We found that acute administration of NT‐4/5 to 7‐day‐old cultures stimulates the hydrolysis of phosphatidylinositol, an event involved in neurotrophin signal transduction. Growth of striatal cultures in the presence of NT‐4/5 resulted in increased cell survival, as indicated by elevations in cell number, protein content, and a measure of mitochondrial enzyme activity (MTT assay). NT‐4/5 increased GABA uptake and staining intensity in these cultures, as indicated by GABA immunocytochemistry, indicating a trophic action on GABAergic neurons, the predominant neuron type in the striatum. To further identify responsive cell populations we analysed for calretinin, a calcium‐binding protein known to colocalize with GABA in a number of neuronal cells. In cultures prepared from rats of embryonic day 15, NT‐4/5 strongly increased the number of calretinin‐positive cells as well as calretinin levels, as determined by Western blot analysis. When the cultures were prepared from embryonic day 18 rats, NT‐4/5 very strongly increased the morphological differentiation of calretinin‐positive cells, whereas the increase in cell number was less prominent. All effects produced by NT‐4/5 were mimicked by BDNF with similar potency. NT‐3 was less effective than NT‐4/5 and BDNF, and its effects were limited to cultures prepared from embryonic day 15 rats, suggesting a role in the regulation of cell survival at early developmental stages. NGF did not affect any of the measured parameters. Our findings identify NT‐4/5 as potent neurotrophic factor for striatal neurons, able to promote their survival and differentiation.

Bibliography

Widmer, H. R., & Hefti, F. (1994). Neurotrophinâ4/5 Promotes Survival and Differentiation of Rat Striatal Neurons Developing in Culture. European Journal of Neuroscience, 6(11), 1669â1679. Portico.

Authors 2

Hans R. Widmer (first)
Franz Hefti (additional)

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Dates

Type	When
Created	19 years, 4 months ago (April 7, 2006, 9:14 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 12:45 a.m.)
Indexed	1 year, 1 month ago (July 30, 2024, 12:33 a.m.)
Issued	30 years, 10 months ago (Nov. 1, 1994)
Published	30 years, 10 months ago (Nov. 1, 1994)
Published Online	19 years, 5 months ago (April 7, 2006)
Published Print	30 years, 10 months ago (Nov. 1, 1994)

Funders 0

None

BibTeX

@article{Widmer_1994, title={Neurotrophin‐4/5 Promotes Survival and Differentiation of Rat Striatal Neurons Developing in Culture}, volume={6}, ISSN={1460-9568}, url={http://dx.doi.org/10.1111/j.1460-9568.1994.tb00559.x}, DOI={10.1111/j.1460-9568.1994.tb00559.x}, number={11}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Widmer, Hans R. and Hefti, Franz}, year={1994}, month=nov, pages={1669–1679} }

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