DOI: 10.1111/j.1460-9568.1992.tb00896.x. Expression of Ciliary Neurotrophic Factor and the Neurotrophins – Nerve Growth Factor, Brain‐Derived Neurotrophic Factor and Neurotrophin 3

Expression of Ciliary Neurotrophic Factor and the Neurotrophins – Nerve Growth Factor, Brain‐Derived Neurotrophic Factor and Neurotrophin 3—in Cultured Rat Hippocampal Astrocytes

10.1111/j.1460-9568.1992.tb00896.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractCultured astrocytes are known to possess a range of neurotrophic activities in culture. In order to examine which factors may be responsible for these activities, we have examined the expression of the genes for four known neurotrophic factors – ciliary neurotrophic factor (CNTF), nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3) – in purified astrocyte cultures derived from neonatal rat hippocampus. Hippocampal astrocytes were found to express mRNA for three neurotrophic factors – CNTF, NGF and NT3 – at significantly higher levels than other cultured cell types or cell lines examined. BDNF messenger RNA (mRNA), however, was undetectable in these astrocytes. The levels of CNTF, NGF and NT3 mRNA in astrocytes were largely unaffected by their degree of confluency, while serum removal caused only a transient decrease in mRNA levels, which returned to basal levels within 48 h. Astrocyte‐derived CNTF was found to comigrate with recombinant rat CNTF at 23 kD on a Western blot. Immunocytochemical analysis revealed strong CNTF immunoreactivity in the cytoplasm of astrocytes, weak staining in the nucleus, but no CNTF at the cell surface. NGF and NT3 were undetectable immunocytochemically. CNTF‐like activity, as assessed by bioassay on ciliary ganglion neurons, was found in the extract of cultured astrocytes but not in conditioned medium, whereas astrocyte‐conditioned medium supported survival of dorsal root ganglion neurons but not ciliary or nodose ganglion neurons. This conditioned medium activity was neutralized with antibodies to NGF. Astrocyte extract also supported survival of dorsal root ganglion and nodose ganglion neurons, but these activities were not blocked by anti‐NGF. Part, but not all, of the activity in astrocyte extracts which sustained nodose ganglion neurons could be attributed to CNTF.

Bibliography

Rudge, J. S., Alderson, R. F., Pasnikowski, E., McClain, J., Ip, N. Y., & Lindsay, R. M. (1992). Expression of Ciliary Neurotrophic Factor and the Neurotrophins â Nerve Growth Factor, BrainâDerived Neurotrophic Factor and Neurotrophin 3âin Cultured Rat Hippocampal Astrocytes. European Journal of Neuroscience, 4(6), 459â471. Portico.

Authors 6

John S. Rudge (first)
Ralph F. Alderson (additional)
Elizabeth Pasnikowski (additional)
Joyce McClain (additional)
Nancy Y. Ip (additional)
Ronald M. Lindsay (additional)

References 70 Referenced 167

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Dates

Type	When
Created	19 years, 4 months ago (April 7, 2006, 8:46 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 7:28 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 7, 2024, 3:17 a.m.)
Issued	33 years, 3 months ago (June 1, 1992)
Published	33 years, 3 months ago (June 1, 1992)
Published Online	19 years, 4 months ago (April 7, 2006)
Published Print	33 years, 3 months ago (June 1, 1992)

Funders 0

None

BibTeX

@article{Rudge_1992, title={Expression of Ciliary Neurotrophic Factor and the Neurotrophins – Nerve Growth Factor, Brain‐Derived Neurotrophic Factor and Neurotrophin 3—in Cultured Rat Hippocampal Astrocytes}, volume={4}, ISSN={1460-9568}, url={http://dx.doi.org/10.1111/j.1460-9568.1992.tb00896.x}, DOI={10.1111/j.1460-9568.1992.tb00896.x}, number={6}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Rudge, John S. and Alderson, Ralph F. and Pasnikowski, Elizabeth and McClain, Joyce and Ip, Nancy Y. and Lindsay, Ronald M.}, year={1992}, month=jun, pages={459–471} }

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