DOI: 10.1111/j.1440-169x.1987.00627.x. Cytological Characterization of Self Incompatibility in Gametes of the Ascidian, <i>Ciona intestinalis</i>

Cytological Characterization of Self Incompatibility in Gametes of the Ascidian, Ciona intestinalis

10.1111/j.1440-169x.1987.00627.x

Crossref journal-article

Wiley

Development, Growth & Differentiation (311)

Abstract

We have determined how many elements are involved in the regulation of self‐fertilization in the solitary ascidian, Ciona intestinalis that is an incompletely self‐sterile species. Animals collected in the field were repeatedly induced to spawn in order to examine their selfing ratios. About 20% of them were self‐fertile, although the ratios fluctuated considerably among respective spawnings. Naturally or acid‐induced self‐fertile gametes required much longer time for selfing than that for crossing. Egg‐suspending seawater (egg water) as such activated sperm motility, but it lowered conspicuously the self‐fertilization ratio. Self‐sterile spermatozoa could scarcely bind to the vitelline coat (VC) of glycerinated autologous eggs. or in case they bound well to it the sperm flagella ceased to beat within five min of the ‘insemination’. The staining of sectioned gametes with DAPI, a fluorescent dye of DNA, showed that in selfing the spermatozoa could hardly penetrate the VC even though they bound well to it. The results of this study show that the block of self‐fertilization can be classified into four elements from a phenomenal viewpoint, such as egg water, low affinity of sperm‐VC binding, inactivation of bound sperm and difficulty in sperm penetration through the VC.

Bibliography

KAWAMURA, K., FUJITA, H., & NAKAUCHI, M. (1987). Cytological Characterization of Self Incompatibility in Gametes of the Ascidian, Ciona intestinalis. Development, Growth & Differentiation, 29(6), 627â642. Portico.

Authors 3

KAZUO KAWAMURA (first)
HIROKO FUJITA (additional)
MITSUAKI NAKAUCHI (additional)

References 34 Referenced 40

10.1007/BF00848815
10.1016/0014-4827(82)90196-3
10.1002/jez.1400780303
10.1016/0014-4827(78)90531-1
10.1002/jez.1400800103
10.1002/jez.1400780304
10.1002/jez.1400850102
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{'key': 'e_1_2_1_16_2', 'first-page': '58', 'volume': '23', 'author': 'Minganti A.', 'year': '1951', 'journal-title': 'Pubbl. Staz. Zool. Napoli'} / Pubbl. Staz. Zool. Napoli by Minganti A. (1951)
10.1038/254244a0
10.1093/icb/22.4.827
10.1093/icb/22.4.841
10.1002/jez.1400800104
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10.1016/0012-1606(81)90322-5
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10.1007/BF01963132
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{'key': 'e_1_2_1_30_2', 'first-page': '107', 'volume-title': 'In The Sperm Cell. Fertilization Power, Surface Properties, Motility, Nucleus and Acrosome, Evolutionary Aspects', 'author': 'Hoshi M.', 'year': '1983'} / In The Sperm Cell. Fertilization Power, Surface Properties, Motility, Nucleus and Acrosome, Evolutionary Aspects by Hoshi M. (1983)
{'key': 'e_1_2_1_31_2', 'first-page': '65', 'volume': '2', 'author': 'Hoshi M.', 'year': '1985', 'journal-title': 'Zool. Sci.'} / Zool. Sci. by Hoshi M. (1985)
{'key': 'e_1_2_1_32_2', 'first-page': '27', 'volume-title': 'In Advances in Invertebrate Reproduction', 'author': 'Hoshi M.', 'year': '1984'} / In Advances in Invertebrate Reproduction by Hoshi M. (1984)
10.1016/0012-1606(80)90449-2
10.1007/BF00848304
10.1016/0012-1606(83)90317-2

Dates

Type	When
Created	20 years, 7 months ago (Jan. 26, 2005, 8:29 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 11:55 p.m.)
Indexed	4 months, 3 weeks ago (April 4, 2025, 2:03 a.m.)
Issued	37 years, 9 months ago (Nov. 1, 1987)
Published	37 years, 9 months ago (Nov. 1, 1987)
Published Online	17 years, 1 month ago (July 7, 2008)
Published Print	37 years, 9 months ago (Nov. 1, 1987)

Funders 0

None

BibTeX

@article{KAWAMURA_1987, title={Cytological Characterization of Self Incompatibility in Gametes of the Ascidian, Ciona intestinalis: (Ciona gametes/self incompatibility/egg water/sperm‐vitelline coat interaction/DAPI staining)}, volume={29}, ISSN={1440-169X}, url={http://dx.doi.org/10.1111/j.1440-169x.1987.00627.x}, DOI={10.1111/j.1440-169x.1987.00627.x}, number={6}, journal={Development, Growth & Differentiation}, publisher={Wiley}, author={KAWAMURA, KAZUO and FUJITA, HIROKO and NAKAUCHI, MITSUAKI}, year={1987}, month=nov, pages={627–642} }

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