DOI: 10.1111/j.1440-1681.1996.tb01167.x. WHERE ARE THE GATES IN GAP JUNCTION CHANNELS?

WHERE ARE THE GATES IN GAP JUNCTION CHANNELS?

10.1111/j.1440-1681.1996.tb01167.x

Crossref journal-article

Wiley

Clinical and Experimental Pharmacology and Physiology (311)

Abstract

SUMMARY1. In the formation and function of gap junction channels two types of gates ought to be discriminated: the docking gate and the channel gates proper. The docking gate is involved in the transformation of a closed hemichannel to a patent gap junction channel. By definition the trigger mechanism for this gate and maybe even the gate itself is contained within the extracellular loops of the gap junction proteins, the connexins. The channel gates proper determine the open and closed states of the complete gap junction channels.2. Probing the docking gate by mutagenesis of connexins and by synthetic peptides indicates that this gate is the consequence of complex interactions between a large fraction of the amino acids comprising the extracellular loops. Probably both inter‐ and intra‐molecular interactions are involved, and disulfide exchange may be entailed in the stabilization of the open and closed states.3. Of the various effectors on the channel gate(s) the voltage effects have obtained the most scrutiny to date. The response of gap junction channels and hemichannels is diverse, the various channels respond differently to transjunctional and membrane potential. No equivalent to the S4 segment representing the voltage sensor in other voltage dependent ion channels is present in the connexin sequences, instead mutations in various segments of connexins have been reported to affect the voltage dependence of gap junction channels. To understand the complexity of voltage effects on gap junction channels, non‐connexin peptides may need to be considered as voltage sensors or as modifiers thereof.

Bibliography

Dahl, G. (1996). WHERE ARE THE GATES IN GAP JUNCTION CHANNELS? Clinical and Experimental Pharmacology and Physiology, 23(12), 1047â1052. Portico.

Authors 1

Gerhard Dahl (first)

References 51 Referenced 18

10.1016/0896-6273(91)90241-Q
10.1126/science.3035715
10.1126/science.2466337
10.1016/0092-8674(89)90180-3
10.1073/pnas.86.14.5380
10.1083/jcb.114.5.1049
{'key': 'e_1_2_1_8_2', 'first-page': '143', 'volume-title': 'Cell‐Cell Interactions. A Practical Approach', 'author': 'Dahl G.', 'year': '1993'} / Cell‐Cell Interactions. A Practical Approach by Dahl G. (1993)
10.1016/S0006-3495(93)81508-X
10.1242/jcs.107.4.955
10.1038/371208a0
10.1016/S0006-3495(92)81804-0
10.1113/jphysiol.1959.sp006143
{'key': 'e_1_2_1_14_2', 'first-page': '1', 'article-title': 'Gap junctions', 'volume': '137', 'author': 'Beyer EC.', 'year': '1993', 'journal-title': 'Int. Rev. Cytol.'} / Int. Rev. Cytol. / Gap junctions by Beyer EC. (1993)
10.1038/329732a0
{'key': 'e_1_2_1_16_2', 'first-page': '2672', 'article-title': 'Molecular organization of gap junctions', 'volume': '43', 'author': 'Revel J‐P', 'year': '1984', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Molecular organization of gap junctions by Revel J‐P (1984)
10.1083/jcb.107.5.1817
{'issue': '19', 'key': 'e_1_2_1_18_2', 'first-page': '105', 'article-title': 'Topology of the Mr 27 000 liver gap junction protein', 'volume': '263', 'author': 'Hertzberg EL', 'year': '1988', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Topology of the Mr 27 000 liver gap junction protein by Hertzberg EL (1988)
10.1002/j.1460-2075.1988.tb03159.x
10.1083/jcb.108.6.2241
10.1242/jcs.100.3.567 / J. Cell Sci. / Topography of connexin32 in rat liver gap junctions. Evidence for an intramolecular disulphide linkage connecting the two extracellular loops by Rahman S. (1991)
10.1007/BF00232671
{'issue': '18', 'key': 'e_1_2_1_23_2', 'first-page': '572', 'article-title': 'Mapping of cystic fibrosis transmembrane conductance regulator membrane topology by glycosylation site insertion', 'volume': '269', 'author': 'Chang X‐B', 'year': '1994', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Mapping of cystic fibrosis transmembrane conductance regulator membrane topology by glycosylation site insertion by Chang X‐B (1994)
10.1016/S0006-3495(94)80663-0
10.1083/jcb.103.1.123
10.1016/0896-6273(89)90235-3
10.1016/S0006-3495(92)81809-X
10.1126/science.2000494
10.1126/science.2000495
10.1083/jcb.115.5.1357
10.1083/jcb.115.4.1077
10.1016/S0006-3495(92)81803-9
10.1016/B978-0-444-89871-5.50008-0
10.1111/j.1432-1033.1991.tb15892.x
10.1016/0006-291X(91)91037-D
10.1098/rspb.1991.0002
10.1006/dbio.1993.1095
10.1016/0014-5793(94)00819-1
10.1073/pnas.88.9.3525
10.1016/S0006-3495(92)81775-7
10.1085/jgp.77.1.95
10.1085/jgp.77.1.77
10.1038/349305a0
10.1038/368348a0
10.1038/365847a0
10.1016/S0006-3495(92)81664-8
10.1146/annurev.ph.47.030185.001433
10.1083/jcb.103.1.135
10.1016/S0006-3495(92)81774-5
10.1016/0896-6273(95)90321-6
{'key': 'e_1_2_1_51_2', 'first-page': '287', 'volume-title': 'Gap Junctions', 'author': 'Bennett MVL', 'year': '1988'} / Gap Junctions by Bennett MVL (1988)
10.1007/BF01870342

Dates

Type	When
Created	18 years, 2 months ago (June 29, 2007, 2:18 a.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 4:20 a.m.)
Indexed	1 year, 10 months ago (Oct. 28, 2023, 1:40 a.m.)
Issued	28 years, 9 months ago (Dec. 1, 1996)
Published	28 years, 9 months ago (Dec. 1, 1996)
Published Online	18 years, 2 months ago (June 28, 2007)
Published Print	28 years, 9 months ago (Dec. 1, 1996)

Funders 0

None

BibTeX

@article{Dahl_1996, title={WHERE ARE THE GATES IN GAP JUNCTION CHANNELS?}, volume={23}, ISSN={1440-1681}, url={http://dx.doi.org/10.1111/j.1440-1681.1996.tb01167.x}, DOI={10.1111/j.1440-1681.1996.tb01167.x}, number={12}, journal={Clinical and Experimental Pharmacology and Physiology}, publisher={Wiley}, author={Dahl, Gerhard}, year={1996}, month=dec, pages={1047–1052} }

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