DOI: 10.1111/j.1439-0523.1992.tb00145.x. Further Characterization of <i>rollC</i> Transgenic Tetraploid Potato Clones, and Influence of Daylength and Level of <i>rolC</i> Expression on Yield Parameters

Further Characterization of rollC Transgenic Tetraploid Potato Clones, and Influence of Daylength and Level of rolC Expression on Yield Parameters

10.1111/j.1439-0523.1992.tb00145.x

Crossref journal-article

Wiley

Plant Breeding (311)

Abstract

AbstractTetraploid rolC transformants have been analyzed considering the level of rolC expression and yield parameters. In rolC transformants a maximum of five integrated copies of the gene were detected by Southern analysis. A relation between copy number of the rolC gene, phenotypic rolC expression and intensity of morphological alterations was established. Three experiments were performed to assess the differential effect of the rolC gene on yield and its interaction with growth factors. In the first experiment, the influence of day length on plant morphological and physiological characters was studied. The phenotype of the transgenic plants with a low rolC expression was like the control under the two day‐regimes tested, while the intermediate expressing genotype showed a different reaction. In the second and third experiment, tuber yield, tuber number and weight per tuber were recorded under short and long day conditions. When grown in smaller pots of 1.15 1 volume, the control plants had the highest yield of tubers under both day lengths tested. Yield decreased with increasing rolC expression. Tuber number revealed to be genotype dependent: it was higher for low rolC expressing plants and lower for intermediate rolC transformants (Experiment 2). In the third experiment the influence of variable pot size and shape was analyzed for one clone with intermediate and two with low level of rolC expression. Remarkable differences between pots and flat boxes of the same volume were noted for tuber yield and number: Under the two conditions the control clone and the low rolC expressing genotypes gave similar tuber yield and number in both growth containers, whereas the rolC intermediate genotype yielded significantly more in flat boxes. The effect of variable hormonal levels induced in transgenic plants through prokaryotic genes are discussed with the existence of non‐predictable genotype in relation to environmental interactions. It is concluded that at least the agronomic performance of potatoes transformed with these genes cannot be predicted on the basis of simple linear relationships among the expression of the gene and of the traits.

Bibliography

Fladung, M., & Ballvora, A. (1992). Further Characterization of rollC Transgenic Tetraploid Potato Clones, and Influence of Daylength and Level of rolC Expression on Yield Parameters. Plant Breeding, 109(1), 18â27. Portico.

Authors 2

M. Fladung (first)
A. Ballvora (additional)

References 29 Referenced 18

10.1073/pnas.81.15.4776
10.1146/annurev.ge.16.120182.002041
10.2307/3868985 / The Plant Cell / Oat phytochrome is biologically active in transgenic tomatoes by Boylan M. T. (1989)
{'key': 'e_1_2_1_5_1', 'first-page': '266', 'article-title': 'A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13. Addendum, 1984', 'volume': '137', 'author': 'Feinberg A. P.', 'year': '1983', 'journal-title': 'Anal. Biochem'} / Anal. Biochem / A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13. Addendum, 1984 by Feinberg A. P. (1983)
10.1111/j.1439-0523.1990.tb00439.x
10.1007/BF00388473
Fourney R. M. J.Miyakoshi R. S.DayIII andM. C.Peterson1988:Northern blotting: Efficient RNA Staining and transfer. Focus 10‐1 5–6.
10.1146/annurev.pp.38.060187.002223 / Ann. Rev. Plant Physiol. / Gibberellin biosynthesis and control by Graebe J. E. (1987)
10.2307/3868986 / The Plant Cell / Rice phytochrome is biologically active in transgenic tobacco by Kay S. A. (1989)
10.1007/BF00339593
10.1007/BF00331014
10.1111/j.1475-4754.1982.tb01001.x
10.1016/0147-619X(79)90059-3
10.1111/j.1399-3054.1962.tb08052.x
Petesek G.1989:Isolation of Pgt‐elicitor inducingc‐DNAs from wheat.Diploma thesis RWTH Aachen.
10.1146/annurev.pp.36.060185.002505 / Ann. Rev. Plant Physiol. / Gibberellins and reproductive development in seed plants by Pharis R. P. (1985)
10.1093/nar/14.14.5857
10.1101/gad.5.3.438
10.1073/pnas.81.24.8014
{'key': 'e_1_2_1_21_1', 'first-page': '455', 'volume-title': 'Nucleic Acids and Proteins in Plants II, Encyclopedia of Plant Physiology', 'author': 'Schell J.', 'year': '1983'} / Nucleic Acids and Proteins in Plants II, Encyclopedia of Plant Physiology by Schell J. (1983)
10.1002/j.1460-2075.1988.tb03114.x
10.1007/BF00017921
10.1016/0304-4211(81)90040-7
10.1002/j.1460-2075.1987.tb02729.x
10.1007/BF00259672
{'key': 'e_1_2_1_27_1', 'first-page': '1', 'article-title': 'Growth, form and composition of potato plants as affected by environment', 'volume': '48', 'author': 'Steward F. C.', 'year': '1981', 'journal-title': 'Ann. Dot.'} / Ann. Dot. / Growth, form and composition of potato plants as affected by environment by Steward F. C. (1981)
10.1073/pnas.79.10.3193
10.1128/jb.164.1.33-44.1985 / Journal Bacteriol. / Molecular and genetic analysis of the transferred DNA regions of the root inducing plasmid of Agrobacterium rhizogenes by White F. F. (1985)
10.1016/0092-8674(83)90289-1

Dates

Type	When
Created	19 years, 4 months ago (April 29, 2006, 2:45 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 5:03 a.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:52 a.m.)
Issued	33 years, 1 month ago (Aug. 1, 1992)
Published	33 years, 1 month ago (Aug. 1, 1992)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	33 years, 1 month ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Fladung_1992, title={Further Characterization of rollC Transgenic Tetraploid Potato Clones, and Influence of Daylength and Level of rolC Expression on Yield Parameters}, volume={109}, ISSN={1439-0523}, url={http://dx.doi.org/10.1111/j.1439-0523.1992.tb00145.x}, DOI={10.1111/j.1439-0523.1992.tb00145.x}, number={1}, journal={Plant Breeding}, publisher={Wiley}, author={Fladung, M. and Ballvora, A.}, year={1992}, month=aug, pages={18–27} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:52:28Z",
    "timestamp": 1754398348819
  },
  "reference-count": 29,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            4,
            28
          ]
        ],
        "date-time": "2006-04-28T00:00:00Z",
        "timestamp": 1146182400000
      },
      "content-version": "vor",
      "delay-in-days": 5018,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Tetraploid <jats:italic>rolC</jats:italic> transformants have been analyzed considering the level of <jats:italic>rolC</jats:italic> expression and yield parameters. In <jats:italic>rolC</jats:italic> transformants a maximum of five integrated copies of the gene were detected by Southern analysis. A relation between copy number of the <jats:italic>rolC</jats:italic> gene, phenotypic <jats:italic>rolC</jats:italic> expression and intensity of morphological alterations was established. Three experiments were performed to assess the differential effect of the <jats:italic>rolC</jats:italic> gene on yield and its interaction with growth factors. In the first experiment, the influence of day length on plant morphological and physiological characters was studied. The phenotype of the transgenic plants with a low <jats:italic>rolC</jats:italic> expression was like the control under the two day\u2010regimes tested, while the intermediate expressing genotype showed a different reaction. In the second and third experiment, tuber yield, tuber number and weight per tuber were recorded under short and long day conditions. When grown in smaller pots of 1.15 1 volume, the control plants had the highest yield of tubers under both day lengths tested. Yield decreased with increasing <jats:italic>rolC</jats:italic> expression. Tuber number revealed to be genotype dependent: it was higher for low <jats:italic>rolC</jats:italic> expressing plants and lower for intermediate <jats:italic>rolC</jats:italic> transformants (Experiment 2). In the third experiment the influence of variable pot size and shape was analyzed for one clone with intermediate and two with low level of <jats:italic>rolC</jats:italic> expression. Remarkable differences between pots and flat boxes of the same volume were noted for tuber yield and number: Under the two conditions the control clone and the low <jats:italic>rolC</jats:italic> expressing genotypes gave similar tuber yield and number in both growth containers, whereas the <jats:italic>rolC</jats:italic> intermediate genotype yielded significantly more in flat boxes. The effect of variable hormonal levels induced in transgenic plants through prokaryotic genes are discussed with the existence of non\u2010predictable genotype in relation to environmental interactions. It is concluded that at least the agronomic performance of potatoes transformed with these genes cannot be predicted on the basis of simple linear relationships among the expression of the gene and of the traits.</jats:p>",
  "DOI": "10.1111/j.1439-0523.1992.tb00145.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        4,
        29
      ]
    ],
    "date-time": "2006-04-29T18:45:42Z",
    "timestamp": 1146336342000
  },
  "page": "18-27",
  "source": "Crossref",
  "is-referenced-by-count": 18,
  "title": "Further Characterization of <i>rollC</i> Transgenic Tetraploid Potato Clones, and Influence of Daylength and Level of <i>rolC</i> Expression on Yield Parameters",
  "prefix": "10.1111",
  "volume": "109",
  "author": [
    {
      "given": "M.",
      "family": "Fladung",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "A.",
      "family": "Ballvora",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        4,
        28
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.81.15.4776"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ge.16.120182.002041"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "765",
      "DOI": "10.2307/3868985",
      "article-title": "Oat phytochrome is biologically active in transgenic tomatoes",
      "volume": "1",
      "author": "Boylan M. T.",
      "year": "1989",
      "journal-title": "The Plant Cell"
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "266",
      "article-title": "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6\u201313. Addendum, 1984",
      "volume": "137",
      "author": "Feinberg A. P.",
      "year": "1983",
      "journal-title": "Anal. Biochem"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1439-0523.1990.tb00439.x"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00388473"
    },
    {
      "key": "e_1_2_1_8_1",
      "unstructured": "Fourney R. M. J.Miyakoshi R. S.DayIII andM. C.Peterson1988:Northern blotting: Efficient RNA Staining and transfer. Focus 10\u20101 5\u20136."
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "419",
      "DOI": "10.1146/annurev.pp.38.060187.002223",
      "article-title": "Gibberellin biosynthesis and control",
      "volume": "38",
      "author": "Graebe J. E.",
      "year": "1987",
      "journal-title": "Ann. Rev. Plant Physiol."
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "775",
      "DOI": "10.2307/3868986",
      "article-title": "Rice phytochrome is biologically active in transgenic tobacco",
      "volume": "1",
      "author": "Kay S. A.",
      "year": "1989",
      "journal-title": "The Plant Cell"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00339593"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00331014"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1475-4754.1982.tb01001.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0147-619X(79)90059-3"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3054.1962.tb08052.x"
    },
    {
      "key": "e_1_2_1_16_1",
      "unstructured": "Petesek G.1989:Isolation of Pgt\u2010elicitor inducingc\u2010DNAs from wheat.Diploma thesis RWTH Aachen."
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1146/annurev.pp.36.060185.002505",
      "article-title": "Gibberellins and reproductive development in seed plants",
      "volume": "36",
      "author": "Pharis R. P.",
      "year": "1985",
      "journal-title": "Ann. Rev. Plant Physiol."
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/14.14.5857"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.5.3.438"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.81.24.8014"
    },
    {
      "key": "e_1_2_1_21_1",
      "first-page": "455",
      "volume-title": "Nucleic Acids and Proteins in Plants II, Encyclopedia of Plant Physiology",
      "author": "Schell J.",
      "year": "1983"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1988.tb03114.x"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00017921"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4211(81)90040-7"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1987.tb02729.x"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00259672"
    },
    {
      "key": "e_1_2_1_27_1",
      "first-page": "1",
      "article-title": "Growth, form and composition of potato plants as affected by environment",
      "volume": "48",
      "author": "Steward F. C.",
      "year": "1981",
      "journal-title": "Ann. Dot."
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.79.10.3193"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "crossref",
      "first-page": "33",
      "DOI": "10.1128/jb.164.1.33-44.1985",
      "article-title": "Molecular and genetic analysis of the transferred DNA regions of the root inducing plasmid of Agrobacterium rhizogenes",
      "volume": "164",
      "author": "White F. F.",
      "year": "1985",
      "journal-title": "Journal Bacteriol."
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(83)90289-1"
    }
  ],
  "container-title": "Plant Breeding",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0523.1992.tb00145.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0523.1992.tb00145.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        23
      ]
    ],
    "date-time": "2023-10-23T09:03:25Z",
    "timestamp": 1698051805000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.1992.tb00145.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  },
  "references-count": 29,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1992,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1439-0523.1992.tb00145.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1439-0523.1992.tb00145.x",
  "relation": {},
  "ISSN": [
    "0179-9541",
    "1439-0523"
  ],
  "subject": [],
  "container-title-short": "Plant Breeding",
  "published": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  }
}