DOI: 10.1111/j.1438-8677.1996.tb00586.x. Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*

Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*

10.1111/j.1438-8677.1996.tb00586.x

Crossref journal-article

Wiley

Botanica Acta (311)

Abstract

Abstract:Specific inhibitors of the secretory pathway represent important tools for investigation of cell wall synthesis and tip growth in pollen tubes. Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM. Ultrastructural investigation of pollen tube cytoplasm showed that brefeldin A caused the appearance of reticular structures and “brefeldin A compartments” containing unesterified pectins. Monensin caused inhibition of pollen tube germination at 80 nM. The drug induced swelling of the Golgi cisternae, many of which contained methyl‐esterified pectins. Cytochalasin D was effective at 1 μg/ml, the inhibition of germination being fully reversible. Application of the drug caused accumulation of secretory vesicles containing methyl‐esterified pectin around the dictyosomes. In contrast to brefeldin A and monensin, cytochalasin D caused a slowdown of cytoplasmic streaming. Monensin, but not the other drugs, caused a considerable decrease in pollen tube diameter. The characterization and quantification of the effects of the drugs on pollen tubes represents a necessary prerequisite for their application in physiological studies.

Bibliography

Geitmann, A., Wojciechowicz, K., & Cresti, M. (1996). Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*. Botanica Acta, 109(5), 373â381. Portico.

Authors 3

Anja Geitmann (first)
Kasia Wojciechowicz (additional)
M. Cresti (additional)

References 49 Referenced 28

10.1002/j.1537-2197.1963.tb06564.x
10.1111/j.1399-3054.1985.tb02318.x
10.1111/j.1438-8677.1993.tb00722.x
10.1111/j.1438-8677.1993.tb00711.x
10.1083/jcb.105.4.1473
10.1111/j.1438-8677.1995.tb00773.x
10.1083/jcb.111.6.2295
{'key': 'e_1_2_1_9_1', 'first-page': '68', 'article-title': 'Metabolism of noncellulosic polysaccharides', 'volume': '13', 'author': 'Fincher G. B.', 'year': '1981', 'journal-title': 'Encycl. Plant Physiol. New Ser'} / Encycl. Plant Physiol. New Ser / Metabolism of noncellulosic polysaccharides by Fincher G. B. (1981)
{'key': 'e_1_2_1_10_1', 'first-page': '217', 'article-title': 'Tubular and filamentous structures in pollen tubes: possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles', 'volume': '105', 'author': 'Franke W. W.', 'year': '1994', 'journal-title': 'Planta'} / Planta / Tubular and filamentous structures in pollen tubes: possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles by Franke W. W. (1994)
Geitmann A. Li Y. ‐Q.andCresti M.The role of the cytoskeleton and dictyosome activity in the pulsatory growth ofNicotiana tabacumandPetunia hybridapollen tubes.Bot. Acta(1996) in press. (10.1111/j.1438-8677.1996.tb00549.x)
10.1007/BF01276814
10.1002/j.1460-2075.1993.tb05727.x
10.1016/S0074-7696(08)61072-4
10.1083/jcb.116.5.1071
10.1007/BF00193004
{'key': 'e_1_2_1_17_1', 'first-page': '153', 'article-title': 'The pollen tube growth test: a new alternative to the Draize eye irritation assay', 'volume': '44', 'author': 'Kristen U.', 'year': '1993', 'journal-title': 'J. Soc. Cosmet. Chem.'} / J. Soc. Cosmet. Chem. / The pollen tube growth test: a new alternative to the Draize eye irritation assay by Kristen U. (1993)
{'key': 'e_1_2_1_18_1', 'first-page': '262', 'article-title': 'Estimation of golgi membrane flow rates in ovary glands of Aptenia cordifolia using Cytochalasin B', 'volume': '29', 'author': 'Kristen U.', 'year': '1983', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / Estimation of golgi membrane flow rates in ovary glands of Aptenia cordifolia using Cytochalasin B by Kristen U. (1983)
10.1007/BF01384453
10.1016/0092-8674(89)90685-5
10.1016/S0040-8166(72)80002-8
10.1007/BF01623957
10.1007/BF01280222
10.1016/0304-4157(90)90008-Z
{'key': 'e_1_2_1_25_1', 'first-page': '25', 'article-title': 'Kinetics of golgi apparatus membrane flux following Monensin treatment of embryogenic carrot cells', 'volume': '30', 'author': 'Morre D. J.', 'year': '1983', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / Kinetics of golgi apparatus membrane flux following Monensin treatment of embryogenic carrot cells by Morre D. J. (1983)
10.1016/0092-8674(91)90517-3
10.1242/jcs.49.1.261 / J. Cell Sci / Determination of secretory vesicle production rates by dictyosomes in pollen tubes of Tradescantia using Cytochalasin D by Picton J. M. (1981)
10.1016/0022-5193(82)90054-6
{'key': 'e_1_2_1_29_1', 'first-page': '133', 'article-title': 'The effect of cycloheximide on dictyosome activity in Tradescantia pollen tubes determined using Cytochalasin D', 'volume': '29', 'author': 'Picton J. M.', 'year': '1983', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / The effect of cycloheximide on dictyosome activity in Tradescantia pollen tubes determined using Cytochalasin D by Picton J. M. (1983)
10.1016/S0074-7696(08)61094-3
10.1007/BF00391581
{'key': 'e_1_2_1_32_1', 'volume-title': 'Plant Membranes', 'author': 'Robinson D. G.', 'year': '1985'} / Plant Membranes by Robinson D. G. (1985)
10.1111/j.1438-8677.1993.tb00345.x
{'key': 'e_1_2_1_34_1', 'first-page': '247', 'article-title': 'Brefeldin A effects on tobacco pollen tubes', 'volume': '61', 'author': 'Rutten T. L. M.', 'year': '1993', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / Brefeldin A effects on tobacco pollen tubes by Rutten T. L. M. (1993)
10.1093/jxb/45.6.685
10.1016/0248-4900(92)90044-2
10.1242/jcs.103.4.1153 / J. Cell Sci / Redistribution of a golgi glycoprotein in plant cells treated with Brefeldin A by Satiat‐Jeunemaitre B. (1992)
10.1006/cbir.1993.1054
10.1007/BF01276269
10.1093/jxb/35.11.1697
10.1093/jxb/35.11.1708
10.1016/S0176-1617(89)80079-3
10.1007/BF00189817
{'key': 'e_1_2_1_44_1', 'first-page': '183', 'volume-title': 'Endocytosis, Exocytosis and Vesicle Traffic in Plants', 'author': 'Staehelin L. A.', 'year': '1991'} / Endocytosis, Exocytosis and Vesicle Traffic in Plants by Staehelin L. A. (1991)
10.1016/B978-0-12-335845-5.50008-2
10.1002/cm.970120404
10.1016/0092-8674(83)90286-6
10.1016/S0074-7696(08)62374-8
{'key': 'e_1_2_1_49_1', 'first-page': '38', 'article-title': 'Effects of Brefeldin A on the golgi complex, endoplasmic reticulum and viral envelope glycoproteins in murine erythroleukemia cells', 'volume': '54', 'author': 'Ulmer J. B.', 'year': '1991', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol / Effects of Brefeldin A on the golgi complex, endoplasmic reticulum and viral envelope glycoproteins in murine erythroleukemia cells by Ulmer J. B. (1991)
10.1104/pp.99.3.1070

Dates

Type	When
Created	11 years, 6 months ago (Feb. 27, 2014, 5:06 a.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 1:26 p.m.)
Indexed	1 year, 10 months ago (Oct. 28, 2023, 5:12 a.m.)
Issued	28 years, 11 months ago (Oct. 1, 1996)
Published	28 years, 11 months ago (Oct. 1, 1996)
Published Online	11 years, 6 months ago (Feb. 27, 2014)
Published Print	28 years, 11 months ago (Oct. 1, 1996)

Funders 0

None

BibTeX

@article{Geitmann_1996, title={Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*}, volume={109}, ISSN={0932-8629}, url={http://dx.doi.org/10.1111/j.1438-8677.1996.tb00586.x}, DOI={10.1111/j.1438-8677.1996.tb00586.x}, number={5}, journal={Botanica Acta}, publisher={Wiley}, author={Geitmann, Anja and Wojciechowicz, Kasia and Cresti, M.}, year={1996}, month=oct, pages={373–381} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        28
      ]
    ],
    "date-time": "2023-10-28T09:12:36Z",
    "timestamp": 1698484356075
  },
  "reference-count": 49,
  "publisher": "Wiley",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2014,
            2,
            27
          ]
        ],
        "date-time": "2014-02-27T00:00:00Z",
        "timestamp": 1393459200000
      },
      "content-version": "vor",
      "delay-in-days": 6358,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        10
      ]
    ]
  },
  "abstract": "<jats:title>Abstract:</jats:title><jats:p>Specific inhibitors of the secretory pathway represent important tools for investigation of cell wall synthesis and tip growth in pollen tubes. Brefeldin A completely inhibits germination of <jats:italic>Nicotiana tabacum</jats:italic> pollen tubes at 2.2 \u03bcM. Ultrastructural investigation of pollen tube cytoplasm showed that brefeldin A caused the appearance of reticular structures and \u201cbrefeldin A compartments\u201d containing unesterified pectins. Monensin caused inhibition of pollen tube germination at 80 nM. The drug induced swelling of the Golgi cisternae, many of which contained methyl\u2010esterified pectins. Cytochalasin D was effective at 1 \u03bcg/ml, the inhibition of germination being fully reversible. Application of the drug caused accumulation of secretory vesicles containing methyl\u2010esterified pectin around the dictyosomes. In contrast to brefeldin A and monensin, cytochalasin D caused a slowdown of cytoplasmic streaming. Monensin, but not the other drugs, caused a considerable decrease in pollen tube diameter. The characterization and quantification of the effects of the drugs on pollen tubes represents a necessary prerequisite for their application in physiological studies.</jats:p>",
  "DOI": "10.1111/j.1438-8677.1996.tb00586.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        2,
        27
      ]
    ],
    "date-time": "2014-02-27T10:06:55Z",
    "timestamp": 1393495615000
  },
  "page": "373-381",
  "source": "Crossref",
  "is-referenced-by-count": 28,
  "title": "Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*",
  "prefix": "10.1111",
  "volume": "109",
  "author": [
    {
      "given": "Anja",
      "family": "Geitmann",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Kasia",
      "family": "Wojciechowicz",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M.",
      "family": "Cresti",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2014,
        2,
        27
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1537-2197.1963.tb06564.x"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3054.1985.tb02318.x"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1438-8677.1993.tb00722.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1438-8677.1993.tb00711.x"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.105.4.1473"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1438-8677.1995.tb00773.x"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.111.6.2295"
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "68",
      "article-title": "Metabolism of noncellulosic polysaccharides",
      "volume": "13",
      "author": "Fincher G. B.",
      "year": "1981",
      "journal-title": "Encycl. Plant Physiol. New Ser"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "217",
      "article-title": "Tubular and filamentous structures in pollen tubes: possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesicles",
      "volume": "105",
      "author": "Franke W. W.",
      "year": "1994",
      "journal-title": "Planta"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Geitmann A. Li Y. \u2010Q.andCresti M.The role of the cytoskeleton and dictyosome activity in the pulsatory growth ofNicotiana tabacumandPetunia hybridapollen tubes.Bot. Acta(1996) in press.",
      "DOI": "10.1111/j.1438-8677.1996.tb00549.x"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01276814"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1993.tb05727.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)61072-4"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.116.5.1071"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00193004"
    },
    {
      "key": "e_1_2_1_17_1",
      "first-page": "153",
      "article-title": "The pollen tube growth test: a new alternative to the Draize eye irritation assay",
      "volume": "44",
      "author": "Kristen U.",
      "year": "1993",
      "journal-title": "J. Soc. Cosmet. Chem."
    },
    {
      "key": "e_1_2_1_18_1",
      "first-page": "262",
      "article-title": "Estimation of golgi membrane flow rates in ovary glands of Aptenia cordifolia using Cytochalasin B",
      "volume": "29",
      "author": "Kristen U.",
      "year": "1983",
      "journal-title": "Eur. J. Cell Biol"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01384453"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90685-5"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0040-8166(72)80002-8"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01623957"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01280222"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4157(90)90008-Z"
    },
    {
      "key": "e_1_2_1_25_1",
      "first-page": "25",
      "article-title": "Kinetics of golgi apparatus membrane flux following Monensin treatment of embryogenic carrot cells",
      "volume": "30",
      "author": "Morre D. J.",
      "year": "1983",
      "journal-title": "Eur. J. Cell Biol"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90517-3"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "crossref",
      "first-page": "261",
      "DOI": "10.1242/jcs.49.1.261",
      "article-title": "Determination of secretory vesicle production rates by dictyosomes in pollen tubes of Tradescantia using Cytochalasin D",
      "volume": "49",
      "author": "Picton J. M.",
      "year": "1981",
      "journal-title": "J. Cell Sci"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-5193(82)90054-6"
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "133",
      "article-title": "The effect of cycloheximide on dictyosome activity in Tradescantia pollen tubes determined using Cytochalasin D",
      "volume": "29",
      "author": "Picton J. M.",
      "year": "1983",
      "journal-title": "Eur. J. Cell Biol"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)61094-3"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00391581"
    },
    {
      "key": "e_1_2_1_32_1",
      "volume-title": "Plant Membranes",
      "author": "Robinson D. G.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1438-8677.1993.tb00345.x"
    },
    {
      "key": "e_1_2_1_34_1",
      "first-page": "247",
      "article-title": "Brefeldin A effects on tobacco pollen tubes",
      "volume": "61",
      "author": "Rutten T. L. M.",
      "year": "1993",
      "journal-title": "Eur. J. Cell Biol"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/jxb/45.6.685"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0248-4900(92)90044-2"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "crossref",
      "first-page": "1153",
      "DOI": "10.1242/jcs.103.4.1153",
      "article-title": "Redistribution of a golgi glycoprotein in plant cells treated with Brefeldin A",
      "volume": "103",
      "author": "Satiat\u2010Jeunemaitre B.",
      "year": "1992",
      "journal-title": "J. Cell Sci"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/cbir.1993.1054"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01276269"
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/jxb/35.11.1697"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/jxb/35.11.1708"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0176-1617(89)80079-3"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00189817"
    },
    {
      "key": "e_1_2_1_44_1",
      "first-page": "183",
      "volume-title": "Endocytosis, Exocytosis and Vesicle Traffic in Plants",
      "author": "Staehelin L. A.",
      "year": "1991"
    },
    {
      "key": "e_1_2_1_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-335845-5.50008-2"
    },
    {
      "key": "e_1_2_1_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cm.970120404"
    },
    {
      "key": "e_1_2_1_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(83)90286-6"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)62374-8"
    },
    {
      "key": "e_1_2_1_49_1",
      "first-page": "38",
      "article-title": "Effects of Brefeldin A on the golgi complex, endoplasmic reticulum and viral envelope glycoproteins in murine erythroleukemia cells",
      "volume": "54",
      "author": "Ulmer J. B.",
      "year": "1991",
      "journal-title": "Eur. J. Cell Biol"
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.99.3.1070"
    }
  ],
  "container-title": "Botanica Acta",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1438-8677.1996.tb00586.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1438-8677.1996.tb00586.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        27
      ]
    ],
    "date-time": "2023-10-27T17:26:37Z",
    "timestamp": 1698427597000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.1996.tb00586.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        10
      ]
    ]
  },
  "references-count": 49,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1996,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1438-8677.1996.tb00586.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1438-8677.1996.tb00586.x",
  "relation": {},
  "ISSN": [
    "0932-8629"
  ],
  "subject": [],
  "container-title-short": "Botanica Acta",
  "published": {
    "date-parts": [
      [
        1996,
        10
      ]
    ]
  }
}