DOI: 10.1111/j.1432-1033.2004.04180.x. Temperature and concentration‐controlled dynamics of rhizobial small heat shock proteins

Temperature and concentration‐controlled dynamics of rhizobial small heat shock proteins

10.1111/j.1432-1033.2004.04180.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A hallmark of α‐crystallin‐type small heat shock proteins (sHsps) is their highly dynamic oligomeric structure which promotes intermolecular interactions involved in subunit exchange and substrate binding (chaperone‐like activity). We studied the oligomeric features of two classes of bacterial sHsps by size exclusion chromatography and nanoelectrospray mass spectrometry. Proteins of both classes formed large complexes that rapidly dissociated upon dilution and at physiologically relevant heat shock temperatures. As the secondary structure was not perturbed, temperature‐ and concentration‐dependent dissociations were fully reversible. Complexes formed between sHsps and the model substrate citrate synthase were stable and exceeded the size of sHsp oligomers. Small Hsps, mutated in a highly conserved glycine residue at the C‐terminal end of the α‐crystallin domain, formed labile complexes that disassembled more readily than the corresponding wild‐type proteins. Reduced complex stability coincided with reduced chaperone activity.

Bibliography

Lentze, N., Aquilina, J. A., Lindbauer, M., Robinson, C. V., & Narberhaus, F. (2004). Temperature and concentrationâcontrolled dynamics of rhizobial small heat shock proteins. European Journal of Biochemistry, 271(12), 2494â2503. Portico.

Authors 5

Nicolas Lentze (first)
J. Andrew Aquilina (additional)
Mareike Lindbauer (additional)
Carol V. Robinson (additional)
Franz Narberhaus (additional)

References 42 Referenced 36

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Dates

Type	When
Created	21 years, 2 months ago (June 4, 2004, 9:16 a.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 8:21 a.m.)
Indexed	2 hours, 37 minutes ago (Aug. 30, 2025, 1:13 p.m.)
Issued	21 years, 2 months ago (June 1, 2004)
Published	21 years, 2 months ago (June 1, 2004)
Published Online	21 years, 2 months ago (June 4, 2004)
Published Print	21 years, 2 months ago (June 1, 2004)

Funders 0

None

BibTeX

@article{Lentze_2004, title={Temperature and concentration‐controlled dynamics of rhizobial small heat shock proteins}, volume={271}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.2004.04180.x}, DOI={10.1111/j.1432-1033.2004.04180.x}, number={12}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Lentze, Nicolas and Aquilina, J. Andrew and Lindbauer, Mareike and Robinson, Carol V. and Narberhaus, Franz}, year={2004}, month=jun, pages={2494–2503} }

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