DOI: 10.1111/j.1432-1033.1997.01111.x. The Subunit F of Mitochondrial Yeast ATP Synthase

The Subunit F of Mitochondrial Yeast ATP Synthase

10.1111/j.1432-1033.1997.01111.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The subunit f of the yeast F1 F0 ATP synthase has been isolated from the purified enzyme. Amino acid composition, protein and peptide sequencing were performed. The data are in agreement with the sequence of the predicted product of the gene D9481.21 identified on the Saccharomyces cerevisiae chromosome IV. A 303‐bp open reading frame encoding a 101‐amino acid polypeptide is described. The deduced amino acid sequence from the ATP 17 gene is 6 amino acids longer than the mature protein, which displays a molecular mass of 10567 Da. The protein is basic with a short hydrophobic segment located in the C‐terminal part of the subunit. Subunit f remained associated with other F0 subunits upon sodium bromide treatment of the whole enzyme. A null mutant was constructed. The disrupted strain was unable to grow on glycerol medium and the mutation was recessive; rho− cells arose spontaneously. The null mutant mitochondria were devoid of oligomycin‐sensitive ATPase, but still contained an active F1 while the subunits f, 6 and 8 were absent.

Bibliography

Spannagel, C., Vaillier, J., Arselin, G., Graves, P., & Velours, J. (1997). The Subunit F of Mitochondrial Yeast ATP Synthase. European Journal of Biochemistry, 247(3), 1111â1117. Portico.

Authors 5

Christelle Spannagel (first)
Jacques Vaillier (additional)
Geneviève Arselin (additional)
Pierre‐Vincent Graves (additional)
Jean Velours (additional)

References 34 Referenced 40

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10.1007/978-3-662-10367-8_3 / The mycota III, biochemistry and molecular biology by Bowman B. J. (1996)
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10.1016/0378-1119(87)90131-4
10.1016/S0021-9258(19)39321-4 / J. Biol. Chem. / Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an inhibitor in yeast by Ichikawa N. (1990)
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Dates

Type	When
Created	21 years, 1 month ago (July 16, 2004, 7:15 a.m.)
Deposited	1 year, 10 months ago (Oct. 31, 2023, 2:22 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 6:35 a.m.)
Issued	28 years, 1 month ago (Aug. 1, 1997)
Published	28 years, 1 month ago (Aug. 1, 1997)
Published Online	21 years, 1 month ago (July 16, 2004)
Published Print	28 years, 1 month ago (Aug. 1, 1997)

Funders 0

None

BibTeX

@article{Spannagel_1997, title={The Subunit F of Mitochondrial Yeast ATP Synthase: Characterization of the Protein and Disruption of the Structural Gene ATP17}, volume={247}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1997.01111.x}, DOI={10.1111/j.1432-1033.1997.01111.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Spannagel, Christelle and Vaillier, Jacques and Arselin, Geneviève and Graves, Pierre‐Vincent and Velours, Jean}, year={1997}, month=aug, pages={1111–1117} }

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