DOI: 10.1111/j.1432-1033.1997.00848.x. The 70‐kDa Heat‐Shock Protein/DnaK Chaperone System is Required for the Productive Folding of Ribulose‐Bisphosphate Carboxylase Subunits in <i>Escherichia Coli</i>

The 70‐kDa Heat‐Shock Protein/DnaK Chaperone System is Required for the Productive Folding of Ribulose‐Bisphosphate Carboxylase Subunits in Escherichia Coli

10.1111/j.1432-1033.1997.00848.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

We have studied the in vivo requirements of the DnaK chaperone system for the folding of recombinant ribulose‐bisphosphate carboxylase/oxygenase in Escherichia coli. Expression of functional dimeric or hexadecameric ribulose‐bisphosphate carboxylase from different bacterial sources (including purple bacteria and cyanobacteria) was severely impaired in E. coli dnaK, dnaJ, or grpE mutants. These enzymes were synthesized mostly in soluble, fully enzymatically active forms in wild‐type E. coli cells cultured in the temperature range 20–42°C, but aggregated extensively in dnaK null mutants. Co‐expression of dnaK, but not groESL, markedly reduced the aggregation of ribulose‐bisphosphate carboxylase subunits in dnaK null mutants and restored the enzyme activity to levels found in isogenic wild‐type strains. Ribulose‐bisphosphate carboxylase expression in wild‐type E. coli cells growing at 30°C promoted an enhanced synthesis of stress proteins, apparently by sequestering DnaK from its negative regulatory role in this response. The overall results indicate that the DnaK chaperone system assists in vivo the folding pathway of ribulose‐bisphosphate carboxylase large subunits, most probably at its very early stages.

Bibliography

Checa, S. K., & Viale, A. M. (1997). The 70âkDa HeatâShock Protein/DnaK Chaperone System is Required for the Productive Folding of RibuloseâBisphosphate Carboxylase Subunits in Escherichia Coli. European Journal of Biochemistry, 248(3), 848â855. Portico.

Authors 2

Susana K. Checa (first)
Alejandro M. Viale (additional)

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Dates

Type	When
Created	21 years, 1 month ago (July 16, 2004, 5:16 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 4:19 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 8:59 a.m.)
Issued	27 years, 11 months ago (Sept. 1, 1997)
Published	27 years, 11 months ago (Sept. 1, 1997)
Published Online	21 years, 1 month ago (July 16, 2004)
Published Print	27 years, 11 months ago (Sept. 1, 1997)

Funders 0

None

BibTeX

@article{Checa_1997, title={The 70‐kDa Heat‐Shock Protein/DnaK Chaperone System is Required for the Productive Folding of Ribulose‐Bisphosphate Carboxylase Subunits in Escherichia Coli}, volume={248}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1997.00848.x}, DOI={10.1111/j.1432-1033.1997.00848.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Checa, Susana K. and Viale, Alejandro M.}, year={1997}, month=sep, pages={848–855} }

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