DOI: 10.1111/j.1432-1033.1997.00812.x. Effects of Brefeldin A on Phosphatidylcholine Phospholipase D and Inositolphospholipid Metabolism in HL‐60 Cells

Effects of Brefeldin A on Phosphatidylcholine Phospholipase D and Inositolphospholipid Metabolism in HL‐60 Cells

10.1111/j.1432-1033.1997.00812.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The involvement of the small GTP‐binding protein ADP‐ribosylation factor (ARF) in guanosine 5′‐[γ‐thio]triphosphate (GTP[S])‐dependent activation of phospholipase D (PLD) in HL‐60 cells has been well established in vitro. In this study, we tested the effect of brefeldin A, which prevents ARF activation by inhibiting guanine‐nucleotide‐exchange activity, on PLD stimulation by receptor agonists (formyl‐Met‐Leu‐Phe and ATP) and by the phorbol ester phorbol 12‐myristate 13‐acetate (PMA) in differentiated HL‐60 cells. However, brefeldin A did not affect the activation of PLD at a time (1 h) when it eliminated the activity of the trans‐Golgi enzyme galactosyltransferase. It also did not inhibit PLD activity in Golgienriched membranes treated with GTP[S] with or without ARF in vitro. However, longer times of brefeldin A treatment (> 6 h), progressively and completely inhibited the activation of PLD by formyl‐Met‐Leu‐Phe and partly inhibited (∼50%) the activation by PMA. In contrast, long‐term brefeldin A treatment did not inhibit the effect of GTP[S] on PLD in permeabilized HL‐60 cells. Long‐term brefeldin A treatment completely inhibited inositol phosphate production in response to formyl‐Met‐Leu‐Phe and ATP, indicating that it affected inositolphospholipid‐specific phospholipase C activity. These data indicate that the rapid inhibitory effect of brefeldin A on Golgi function is not associated with inhibition of receptor‐mediated or PMA‐mediated PLD activation in HL‐60 cells. However, longer‐term effects, presumably arising from the disruption of the Golgi, lead to a total inhibition of agonist activation of PLD and inositolphospholipid‐specific phospholipase C. In summary, these results do not support a role for brefeldin‐A‐sensitive ARF in agonist regulation of PLD in HL‐60 cells.

Bibliography

Guillemain, I., & Exton, J. H. (1997). Effects of Brefeldin A on Phosphatidylcholine Phospholipase D and Inositolphospholipid Metabolism in HLâ60 Cells. European Journal of Biochemistry, 249(3), 812â819. Portico.

Authors 2

Isabella Guillemain (first)
John H. Exton (additional)

References 39 Referenced 22

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{'key': 'e_1_2_2_10_2', 'first-page': '6409', 'article-title': 'ADP‐ribosylation factor, a small GTP‐binding protein, is required for binding of the coatomer protein β‐COP to Golgi membranes', 'volume': '89', 'author': 'Donaldson J. G.', 'year': '1992', 'journal-title': 'Proc. NatlA cad. Sci. USA'} / Proc. NatlA cad. Sci. USA / ADP‐ribosylation factor, a small GTP‐binding protein, is required for binding of the coatomer protein β‐COP to Golgi membranes by Donaldson J. G. (1992)
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10.1038/358512a0

Dates

Type	When
Created	21 years, 2 months ago (June 23, 2004, 11:15 a.m.)
Deposited	1 year, 10 months ago (Oct. 8, 2023, 2:15 p.m.)
Indexed	5 months ago (March 30, 2025, 9:27 a.m.)
Issued	27 years, 10 months ago (Nov. 1, 1997)
Published	27 years, 10 months ago (Nov. 1, 1997)
Published Online	21 years, 2 months ago (June 23, 2004)
Published Print	27 years, 10 months ago (Nov. 1, 1997)

Funders 0

None

BibTeX

@article{Guillemain_1997, title={Effects of Brefeldin A on Phosphatidylcholine Phospholipase D and Inositolphospholipid Metabolism in HL‐60 Cells}, volume={249}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1997.00812.x}, DOI={10.1111/j.1432-1033.1997.00812.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Guillemain, Isabella and Exton, John H.}, year={1997}, month=nov, pages={812–819} }

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