DOI: 10.1111/j.1432-1033.1996.00557.x. Structural and Functional Characterization of Vitronectin‐Derived RGD‐Containing Peptides from Human Hemofiltrate

Structural and Functional Characterization of Vitronectin‐Derived RGD‐Containing Peptides from Human Hemofiltrate

10.1111/j.1432-1033.1996.00557.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Bioactive peptides derived from the adhesive plasma protein vitronectin are present at submicromolar concentrations in human hemofiltrate of patients with renal diseases and were isolated by a combination of high‐efficiency chromatographic steps. The structural and functional properties of these peptides were characterized. Sequencing and mass spectrometry revealed the existence of peptide isoforms (5‐6 kDa) which corresponded to the N‐terminus (residues 1 to 44‐50) of vitronectin. The isolated peptides bound directly to plasminogen‐activator inhibitor‐1 (PAI‐1) and were effective competitors of the interaction of PAI‐1 with isolated intact vitronectin or extracellular matrix. These functional properties were indistinguishable from the binding properties of a recombinant fusion protein containing residues 1‐52 of vitronectin linked to a portion of glutathione S‐transferase, expressed in Escherichia coli. Peptides containing the RGD sequence of vitronectin competed for vitronectin binding to the αvβ3 integrin. No indication for direct growth‐factor binding was noted, whereas natural peptides were found associated with PAI‐1 as the major binding protein in plasma. These data demonstrate that functionally active vitronectin‐derived peptides are released by unknown protease(s) from the mature protein and that these peptides are identical, in terms of activity, to recombinant vitronectin fragments. These natural peptides may interact with active PAI‐1 in plasma or at extravascular sites and thereby interfere with established biological functions of intact vitronectin.

Bibliography

StÃ¤ndker, L., Enger, A., SchulzâKnappe, P., Wohn, K., Germer, M., Raida, M., Forssmann, W., & Preissner, K. T. (1996). Structural and Functional Characterization of VitronectinâDerived RGDâContaining Peptides from Human Hemofiltrate. European Journal of Biochemistry, 241(2), 557â563. Portico.

Authors 8

Ludger Ständker (first)
Andreas Enger (additional)
Peter Schulz‐Knappe (additional)
Karl‐Dieter Wohn (additional)
Matthias Germer (additional)
Manfred Raida (additional)
Wolf‐Georg Forssmann (additional)
Klaus T. Preissner (additional)

References 37 Referenced 20

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10.1016/S0021-9258(18)31646-6

Dates

Type	When
Created	21 years, 1 month ago (July 23, 2004, 5:16 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 9:31 a.m.)
Indexed	1 year, 10 months ago (Oct. 16, 2023, 2:15 a.m.)
Issued	28 years, 11 months ago (Oct. 1, 1996)
Published	28 years, 11 months ago (Oct. 1, 1996)
Published Online	21 years, 1 month ago (July 23, 2004)
Published Print	28 years, 11 months ago (Oct. 1, 1996)

Funders 0

None

BibTeX

@article{St_ndker_1996, title={Structural and Functional Characterization of Vitronectin‐Derived RGD‐Containing Peptides from Human Hemofiltrate}, volume={241}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1996.00557.x}, DOI={10.1111/j.1432-1033.1996.00557.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Ständker, Ludger and Enger, Andreas and Schulz‐Knappe, Peter and Wohn, Karl‐Dieter and Germer, Matthias and Raida, Manfred and Forssmann, Wolf‐Georg and Preissner, Klaus T.}, year={1996}, month=oct, pages={557–563} }

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