DOI: 10.1111/j.1432-1033.1995.766_3.x. The Functional Integration of a Polytopic Membrane Protein of <i>Escherichia coli</i> is Dependent on the Bacterial Signal‐Recognition Particle

The Functional Integration of a Polytopic Membrane Protein of Escherichia coli is Dependent on the Bacterial Signal‐Recognition Particle

10.1111/j.1432-1033.1995.766_3.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

In eukaryotes, the cotranslational targeting of proteins to the endoplasmic reticular membrane is initially mediated by the signal‐recognition particle (SRP), a ribonucleoprotein complex consisting of the 7SL RNA and six protein subunits. Since the discovery of sequence homology between (a) the Escherichia coli 4.5s RNA (Ffs) and 7SL RNA, and (b) the E. coli P48 (Ffh) and SRP 54‐kDa subunit, more evidence has been obtained that E. coli also possesses an SRP‐type pathway that acts in the translocation of secreted proteins. Such a pathway could possibly be involved in the cotranslational integration of hydrophobic membrane proteins that cannot be effectively targeted post‐translationally due to folding and aggregation. In this study, we report that disruption of the E. coli SRP complex with a dominant lethal 4.5s RNA mutant in vivo prevents functional membrane integration of the E. coli lactose permease (Lacue). Likewise, depletion of the P48 (Ffh) protein also results in a decrease in the amount of functional Laue inserted into the E. coli plasma membrane. In direct contrast, inhibition of SecA function does not affect Laue integration. These results suggest a major function of the bacterial SRP in the targeting and subsequent integration of hydrophobic membrane proteins as opposed to SecA mediating the post‐translational targeting of secretory proteins.

Bibliography

Macfarlane, J., & MÃ¼ller, M. (1995). The Functional Integration of a Polytopic Membrane Protein of Escherichia coli is Dependent on the Bacterial SignalâRecognition Particle. European Journal of Biochemistry, 233(3), 766â771. Portico.

Authors 2

Juan Macfarlane (first)
Matthias Müller (additional)

References 32 Referenced 90

10.1007/BF00763169
10.1128/mr.53.3.333-366.1989 / Microbiol. Rev. / Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eukaryotic process by Saier M. H. (1989)
10.1146/annurev.ge.24.120190.001243
10.1146/annurev.bi.60.070191.000533
{'key': 'e_1_2_3_6_2', 'first-page': '327', 'volume-title': 'Subcellular biochemistry', 'author': 'Müller M.', 'year': '1994'} / Subcellular biochemistry by Müller M. (1994)
10.1016/0092-8674(90)90111-Q
10.1002/j.1460-2075.1993.tb06015.x
10.1016/0092-8674(88)90003-7
10.1038/340478a0
10.1038/340482a0
10.1016/0092-8674(90)90454-M
10.1126/science.1701272
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10.1002/j.1460-2075.1994.tb06511.x
10.1038/359744a0
10.1038/367657a0
10.1073/pnas.90.11.5229
10.1016/S0021-9258(18)54790-6 / J. Biol. Chem. / Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery by McCovern K. (1991)
10.1016/S0021-9258(18)35796-X
10.1111/j.1432-1033.1992.tb17111.x
10.1111/j.1365-2958.1991.tb02154.x
10.1093/oxfordjournals.jbchem.a123777
10.1083/jcb.108.5.1637
{'key': 'e_1_2_3_25_2', 'first-page': '117', 'article-title': 'The role of permease in transport', 'volume': '79', 'author': 'Koch A. L.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta.'} / Biochim. Biophys. Acta. / The role of permease in transport by Koch A. L. (1964)
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10.1128/jb.113.2.798-812.1973 / J. Bacteriol. / Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K12 by Low K. B. (1973)
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{'key': 'e_1_2_3_30_2', 'first-page': '45', 'volume-title': 'The lactose operon', 'author': 'Kennedy E. P.', 'year': '1970'} / The lactose operon by Kennedy E. P. (1970)
10.1073/pnas.60.2.725
10.1007/BF00763170
10.1016/0092-8674(93)90483-7

Dates

Type	When
Created	20 years, 11 months ago (Sept. 7, 2004, 8:20 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 4:12 p.m.)
Indexed	3 months, 1 week ago (May 18, 2025, 10:45 a.m.)
Issued	29 years, 9 months ago (Nov. 1, 1995)
Published	29 years, 9 months ago (Nov. 1, 1995)
Published Online	20 years, 11 months ago (Sept. 7, 2004)
Published Print	29 years, 9 months ago (Nov. 1, 1995)

Funders 0

None

BibTeX

@article{Macfarlane_1995, title={The Functional Integration of a Polytopic Membrane Protein of Escherichia coli is Dependent on the Bacterial Signal‐Recognition Particle}, volume={233}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1995.766_3.x}, DOI={10.1111/j.1432-1033.1995.766_3.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Macfarlane, Juan and Müller, Matthias}, year={1995}, month=nov, pages={766–771} }

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