DOI: 10.1111/j.1432-1033.1995.443_b.x. Purification and Two‐Dimensional Crystallization of Bacterial Cytochrome Oxidases

Purification and Two‐Dimensional Crystallization of Bacterial Cytochrome Oxidases

10.1111/j.1432-1033.1995.443_b.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A novel strategy which employes chromatography on an immobilized metal ion has been developed for the purification of bacterial cytochrome c and quinol oxidases. Many bacterial oxidase complexes appear to have a natural affinity to bind to the chelated copper ion. A combination of three different chromatographic principles (anion exchange, metal‐affinity and gel filtration) makes an effective tool chest for the preparation of homogeneous and protein‐chemically pure bacterial oxidases. These preparations have been used for two‐dimensional crystallization. Until now, crystals have been obtained using the Paracoccus denitrificans and Rhodobacter sphaeroides cytochrome aa3, and the Escherichia coli cytochrome bo. The crystals diffract to approximately 2.5 nm in negative stain and have potential for further structural studies.

Bibliography

Warne, A., Wang, D. N., & Saraste, M. (1995). Purification and TwoâDimensional Crystallization of Bacterial Cytochrome Oxidases. European Journal of Biochemistry, 234(2), 443â451. Portico.

Authors 3

Antony Warne (first)
Da Neng Wang (additional)
Matti Saraste (additional)

References 51 Referenced 16

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10.1111/j.1365-2958.1994.tb00414.x
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10.1016/S0021-9258(18)35760-0 / J. Biol. Chem. / Cytochrome aa 3 of Rhodobacter sphaeroides as a model for mitochondrial cytochrome c oxidase: Purification, kinetics, proton pumping, and spectral analysis by Hosler J. P. (1992)
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Dates

Type	When
Created	20 years, 11 months ago (Sept. 6, 2004, 10:43 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 10:06 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:08 a.m.)
Issued	29 years, 9 months ago (Dec. 1, 1995)
Published	29 years, 9 months ago (Dec. 1, 1995)
Published Online	20 years, 11 months ago (Sept. 6, 2004)
Published Print	29 years, 9 months ago (Dec. 1, 1995)

Funders 0

None

BibTeX

@article{Warne_1995, title={Purification and Two‐Dimensional Crystallization of Bacterial Cytochrome Oxidases}, volume={234}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1995.443_b.x}, DOI={10.1111/j.1432-1033.1995.443_b.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Warne, Antony and Wang, Da Neng and Saraste, Matti}, year={1995}, month=dec, pages={443–451} }

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