DOI: 10.1111/j.1432-1033.1994.tb19913.x. Lead‐ion‐induced cleavage of RNase P RNA

Lead‐ion‐induced cleavage of RNase P RNA

10.1111/j.1432-1033.1994.tb19913.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Pb2+‐induced hydrolysis of RNase P RNAs from Escherichia coli and the thermophilic eubacterium Thermus thermophilus HB8 revealed one prominent site‐specific cleavage in the two RNAs and several minor cleavage sites in structurally corresponding regions of both RNAs. Data presented here and in a previous study [Kazakov, S. & Altman, S. (1991) Proc. Natl Acad. Sci. USA 88, 9193–9197] provide evidence for several ubiquitous metal‐ion‐binding sites in eubacterial RNase P RNA subunits. With the T. thermophilus RNase P RNA, susceptibility to Pb2+‐induced strand scission at the most prominent site was hypersensitive at the temperature of highest enzyme activity (55°C). Pb2+ hydrolysis at this site was strongly reduced at a temperature of 37°C, where processing is also inefficient. For E. coli RNase P RNA, specific changes in the lead hydrolysis pattern were observed due to the presence of excess tRNA. Thus, Pb2+‐induced hydrolysis seems suitable to sense different conformations of RNase P RNAs. The T. thermophilus RNase P RNA, in particular, displayed significant processing activity after severe fragmentation by Pb2+, and therefore appears to be suited for reconstituting an active enzyme from RNA subfragments.

Bibliography

CIESIOLKA, J., HARDT, W., SCHLEGL, J., ERDMANN, V. A., & HARTMANN, R. K. (1994). Leadâionâinduced cleavage of RNase P RNA. European Journal of Biochemistry, 219(1â2), 49â56. Portico.

Authors 5

Jerzy CIESIOLKA (first)
Wolf‐Dietrich HARDT (additional)
Judith SCHLEGL (additional)
Volker A. ERDMANN (additional)
Roland K. HARTMANN (additional)

References 33 Referenced 64

10.1021/bi00462a013
10.1038/303543a0
10.1021/bi00339a012
10.1093/nar/20.7.1451
10.1111/j.1432-1033.1992.tb16670.x
10.1111/j.1432-1033.1992.tb16671.x
10.1021/bi00117a003
10.1093/nar/21.3.531
10.1038/275560a0
10.1016/0092-8674(83)90117-4
10.1021/bi00355a006
10.1073/pnas.89.4.1266
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10.1093/nar/21.15.3521
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10.1128/jb.173.8.2681-2690.1991
10.1016/0022-2836(78)90210-3
10.1073/pnas.88.20.9193
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10.1126/science.1720569
10.1021/bi00131a001
10.1016/0022-2836(91)80194-Y
10.1126/science.3122322
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10.1093/nar/20.22.5963
10.1016/S0021-9258(18)45897-8 / J. Biol. Chem. / Influence of metal ions on the ribonuclease P reaction by Smith D. (1992)
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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 4:49 a.m.)
Deposited	1 year, 11 months ago (Aug. 31, 2023, 3:54 a.m.)
Indexed	1 year, 2 months ago (June 26, 2024, 8:54 p.m.)
Issued	31 years, 7 months ago (Jan. 1, 1994)
Published	31 years, 7 months ago (Jan. 1, 1994)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	31 years, 7 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{CIESIOLKA_1994, title={Lead‐ion‐induced cleavage of RNase P RNA}, volume={219}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1994.tb19913.x}, DOI={10.1111/j.1432-1033.1994.tb19913.x}, number={1–2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={CIESIOLKA, Jerzy and HARDT, Wolf‐Dietrich and SCHLEGL, Judith and ERDMANN, Volker A. and HARTMANN, Roland K.}, year={1994}, month=jan, pages={49–56} }

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