DOI: 10.1111/j.1432-1033.1994.tb18906.x. The genetic interaction of <i>kar2</i> and <i>wbp1</i> mutations

The genetic interaction of kar2 and wbp1 mutations

10.1111/j.1432-1033.1994.tb18906.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The endoplasmic binding protein BiP and N‐linked glycosylation are proposed to be essential components in the processing pathway of secreted protein. In Saccharomyces cerevisiae, BiP is encoded by the KAR2 gene; WBP1 encodes an essential component of the N‐oligosaccharyltransferase complex. wbp1 mutations result in reduced oligosaccharyltransferase activity and a temperature‐sensitive phenotype. We show that a combination of kar2 and wbp1 mutations results in a synthetic phenotype with a strongyl reduced growth rate at the permissive temperature. To investigate the role of N‐linked glycosylation in BiP function, the processing of non‐glycosylated carboxypeptidase was followed in different kar2 strains at the permissive temperature. In all kar2 strains, the processing of non‐glycosylated carboxypeptidase Y was drastically reduced. A specific BiP/non‐glycosylated carboxypeptidase Y complex was detected in kar2‐159 and kar2‐203 cells whereas the kar2‐1 mutation did not result in such a complex. Our data show that BiP and N‐linked glycosylation are directly involved in the processing of secreted proteins. The results support the hypothesis that BiP stabilizes the folding‐competent and assembly‐competent state of a polypeptide, whereas N‐linked oligosaccharides are structural components required in the folding process after the polypeptide is released from BiP.

Bibliography

TE HEESEN, S., & AEBI, M. (1994). The genetic interaction of kar2 and wbp1 mutations. European Journal of Biochemistry, 222(2), 631â637. Portico.

Authors 2

Stephan TE HEESEN (first)
Markus AEBI (additional)

References 34 Referenced 18

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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 5:19 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:39 a.m.)
Indexed	3 months, 2 weeks ago (May 13, 2025, 9:31 p.m.)
Issued	31 years, 2 months ago (June 1, 1994)
Published	31 years, 2 months ago (June 1, 1994)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	31 years, 2 months ago (June 1, 1994)

Funders 0

None

BibTeX

@article{TE_HEESEN_1994, title={The genetic interaction of kar2 and wbp1 mutations: Distinct functions of binding protein BiP and N‐linked glycosylation in the processing pathway of secreted proteins in Saccharomyces cerevisiae}, volume={222}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1994.tb18906.x}, DOI={10.1111/j.1432-1033.1994.tb18906.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={TE HEESEN, Stephan and AEBI, Markus}, year={1994}, month=jun, pages={631–637} }

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