DOI: 10.1111/j.1432-1033.1994.tb18608.x. H<sub>2</sub>: heterodisulfide oxidoreductase complex from <i>Methanobacterium thermoautotrophicum</i>

H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum

10.1111/j.1432-1033.1994.tb18608.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The reduction of the heterodisulfide (CoM‐S‐S‐HTP) of coenzyme M (H‐S‐CoM) and N‐7‐mercaptoheptanoylthreonine phosphate (H‐S‐HTP) with H2 is an energy‐conserving step in most methanogenic Archaea. In this study, we show that in Methanobacterium thermoautotrophicum (strain Marburg) this reaction is catalyzed by a stable H2: heterodisulfide oxidoreductase complex of F420‐non‐reducing hydrogenase and heterodisulfide reductase. This complex, which was loosely associated with the cytoplasmic membrane, was purified 17‐fold with 80% yield to apparent homogeneity. The purified complex was composed of six different subunits of apparent molecular masses 80, 51, 41, 36, 21 and 17 kDa, and 1 mol complex, with apparent molecular mass 250 kDa, contained approximately 0.6 mol nickel, 0.9 mol FAD, 26 mol non‐heme iron and 22 mol acid‐labile sulfur. In 25 nM Chaps, the complex partially dissociated into two subcomplexes. The first subcomplex was was composed of the 51‐, 41‐ and 17‐kDa subunits; 1 mol trimer contained 0.7 mol nickel, 10 mol non‐heme iron and 9 mol acid‐labile sulfur and exhibited F420‐non‐reducing hydrogenase activity. The other subcomplex was composed of the 80‐, 36‐ and 21‐kDa subunits; 1 mol trimer contained 0.8 mol FAD, 22 mol non‐heme iron and 15 mol acid‐labile sulfur and exhibited heterodisulfide‐reductase activity. The stimulatory effects of potassium phosphate, a membrane component, uracil derivatives and coenzyme F430 on the H2:heterodisulfide‐oxidoreductase activity of the purified complex are described.

Authors 4

Edgar SETZKE (first)
Reiner HEDDERICH (additional)
Stefanie HEIDEN (additional)
Rudolf K. THAUER (additional)

References 45 Referenced 90

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{'key': 'e_1_2_3_7_1', 'first-page': '311', 'article-title': 'Relatedness of strains ΔH and Marburg of Methanobacterium thermoautotrophicum', 'volume': '2', 'author': 'Brandis A.', 'year': '1981', 'journal-title': 'Zentralbl. Bakteriol. Mikrobiol. Hyg. 1 Abt Orig. C'} / Zentralbl. Bakteriol. Mikrobiol. Hyg. 1 Abt Orig. C / Relatedness of strains ΔH and Marburg of Methanobacterium thermoautotrophicum by Brandis A. (1981)
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{'key': 'e_1_2_3_43_1', 'first-page': '121', 'volume-title': 'Chemistry and biochemistry of flavoenzymes', 'author': 'Williams C. H.', 'year': '1992'} / Chemistry and biochemistry of flavoenzymes by Williams C. H. (1992)
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10.1016/S0021-9258(19)81724-6

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 1:58 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:24 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:24 a.m.)
Issued	31 years, 6 months ago (Feb. 1, 1994)
Published	31 years, 6 months ago (Feb. 1, 1994)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	31 years, 6 months ago (Feb. 1, 1994)

Funders 0

None

BibTeX

@article{SETZKE_1994, title={H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum: Composition and properties}, volume={220}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1994.tb18608.x}, DOI={10.1111/j.1432-1033.1994.tb18608.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SETZKE, Edgar and HEDDERICH, Reiner and HEIDEN, Stefanie and THAUER, Rudolf K.}, year={1994}, month=feb, pages={139–148} }

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