DOI: 10.1111/j.1432-1033.1994.00541.x. Molecular Characterization of <i>Escherichia coli</i> Malate Synthase G

Molecular Characterization of Escherichia coli Malate Synthase G

10.1111/j.1432-1033.1994.00541.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Two genes encoding the enzymes malate synthase G and glycolate oxidase, have been linked to locus glc (64.5 min), responsible for glycolate utilization in Escherichia coli. The gene encoding malate synthase G, for which we propose the notation glcB, has been cloned, sequenced and found to correspond to a 2262‐nucleotide open‐reading frame, which can encode a 723‐amino‐acid polypeptide, clearly different from the isoenzyme malate synthase A, which has 533 amino acids. Northern‐blot experiments indicate that glcB was expressed as an apparently monocistronic transcript, inducible by glycolate. Malate synthase G was purified to near homogeneity. The molecular mass determined by gel filtration yielded a value of 82 kDa for the purified enzyme and the same value as for the crude extract enzyme, indicating a monomeric structure. Despite the lower sequence similarity between malate synthase G and the other reported malate synthases, three out of nine consensus boxes defined in most of these enzymes are conserved in addition to a cysteine residue that has been reported to be important for the catalytic mechanisms.

Bibliography

Molina, I., Pellicer, M., Badia, J., Aguilar, J., & Baldoma, L. (1994). Molecular Characterization of Escherichia coli Malate Synthase G. European Journal of Biochemistry, 224(2), 541â548. Portico.

Authors 5

Isabel Molina (first)
María‐Teresa Pellicer (additional)
Josefa Badia (additional)
Juan Aguilar (additional)
Laura Baldoma (additional)

References 30 Referenced 57

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{'key': 'e_1_2_3_6_2', 'first-page': '221', 'article-title': 'Glycolate oxidoreductase in Escherichia coli', 'volume': '267', 'author': 'Lord J. M.', 'year': '1972', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta / Glycolate oxidoreductase in Escherichia coli by Lord J. M. (1972)
10.1128/jb.81.4.509-518.1961 / J. Bacteriol. / Glyoxylic acid carboligase. An enzyme present in glycolate‐grown Escherichia coli by Krakow G. (1961)
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10.1128/jb.147.1.181-185.1981 / J. Bacteriol. / Metabolism of l‐fucose and l‐rhamnose in Escherichia coli: Differences in induction of propanediol oxidoreductase by Boronat A. (1981)
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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 6:04 a.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 5:09 p.m.)
Indexed	4 months, 2 weeks ago (April 15, 2025, 3:33 a.m.)
Issued	31 years ago (Sept. 1, 1994)
Published	31 years ago (Sept. 1, 1994)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	31 years ago (Sept. 1, 1994)

Funders 0

None

BibTeX

@article{Molina_1994, title={Molecular Characterization of Escherichia coli Malate Synthase G: Differentiation with the Malate Synthase A Isoenzyme}, volume={224}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1994.00541.x}, DOI={10.1111/j.1432-1033.1994.00541.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Molina, Isabel and Pellicer, María‐Teresa and Badia, Josefa and Aguilar, Juan and Baldoma, Laura}, year={1994}, month=sep, pages={541–548} }

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