DOI: 10.1111/j.1432-1033.1994.00213.x. Importance of General Regulatory Factors Rap1p, Abf1p and Reb1p for the Activation of Yeast Fatty Acid Synthase Genes <i>FAS1</i> and <i>FAS2</i>

Importance of General Regulatory Factors Rap1p, Abf1p and Reb1p for the Activation of Yeast Fatty Acid Synthase Genes FAS1 and FAS2

10.1111/j.1432-1033.1994.00213.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The fatty acid synthase genes FAS1 and FAS2 of the yeast Saccharomyces cerevisiae are under transcriptional control of pathway‐specific regulators of phospholipid biosynthesis. However, site‐directed mutagenesis of the respective cis ‐acting elements upstream of FAS1 and FAS2 revealed that additional sequences activating both genes must exist. A deletion analysis of the FAS1 promoter lacking the previously characterized inositol/choline‐responsive‐element motif defined a region (nucleotides –760 to –850) responsible for most of the remaining activation potency. Gel‐retardation experiments and in‐vitro DNase footprint studies proved the binding of the general regulatory factors Rap1p, Abf1p and Reb1p to this FAS1 upstream region. Mutation of the respective binding sites led to a drop of gene activation to 8% of the wild‐type level. Similarly, we also demonstrated the presence of a Reb1p‐binding site upstream of FAS2 and its importance for gene activation. Thus, in addition to the previously characterized FAS‐binding factor 1 interacting with the inositol/choline‐responsive‐element motif, a second motif common to the promoter regions of both FAS genes could be identified. Transcription of yeast fatty acid synthase genes is therefore subjected to both the pathway‐specific control affecting genes of phospholipid biosynthesis and to the activation by general transcription factors allowing a sufficiently high level of constitutive gene expression.

Bibliography

SchÃ¼ller, H., SchÃ¼tz, A., Knab, S., Hoffmann, B., & Schweizer, E. (1994). Importance of General Regulatory Factors Rap1p, Abf1p and Reb1p for the Activation of Yeast Fatty Acid Synthase Genes FAS1 and FAS2. European Journal of Biochemistry, 225(1), 213â222. Portico.

Authors 5

Hans‐Joachim Schüller (first)
Armin Schütz (additional)
Sabine Knab (additional)
Brigitte Hoffmann (additional)
Eckhart Schweizer (additional)

References 53 Referenced 34

10.1083/jcb.117.3.515
{'key': 'e_1_2_3_3_2', 'first-page': '3', 'volume-title': 'Microbial Lipids', 'author': 'Schweizer E.', 'year': '1989'} / Microbial Lipids by Schweizer E. (1989)
10.1007/BF00422073
{'key': 'e_1_2_3_5_2', 'first-page': '570', 'article-title': 'Genetic control of fatty acid synthetase biosynthesis and structure in lower fungi', 'volume': '89', 'author': 'Schweizer E.', 'year': '1987', 'journal-title': 'Fat Sci. Technol.'} / Fat Sci. Technol. / Genetic control of fatty acid synthetase biosynthesis and structure in lower fungi by Schweizer E. (1987)
10.1016/S0021-9258(18)61337-7 / J. Biol. Chem. / Complementation of mutations and nucleotide sequence of FAS1 gene encoding β subunit of yeast fatty acid synthase by Chirala S. S. (1987)
10.1016/S0021-9258(18)37757-3 / J. Biol. Chem. / Primary structure of the multifunctional α subunit protein of yeast fatty acid synthase derived from FAS2 gene sequence by Mohamed A. H. (1988)
10.1007/BF00273618
10.1002/j.1460-2075.1992.tb05033.x
10.1093/nar/20.22.5955
10.1073/pnas.83.22.8516
10.1126/science.3303332 / Science / Multiple global regulation control of HIS4 transcription in yeast by Arndt K. (1987)
10.1128/MCB.11.7.3642
{'key': 'e_1_2_3_14_2', 'first-page': '2050', 'article-title': 'The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions', 'volume': '9', 'author': 'Vogel K.', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions by Vogel K. (1989)
10.1073/pnas.89.15.6746 / Proc. Natl Acad. Sci. USA / Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2 by Daignan‐Fornier B. (1992)
10.1016/0092-8674(87)90095-X
10.1128/MCB.8.1.210
10.1101/gad.5.4.616
10.1126/science.2237406
10.1002/j.1460-2075.1987.tb02386.x
10.1002/j.1460-2075.1985.tb04114.x
10.1128/MCB.9.12.5516
10.1093/nar/18.23.7099
10.1016/0378-1119(90)90441-S
10.1101/gad.3.12a.1926
10.1002/j.1460-2075.1989.tb08453.x
10.1073/pnas.88.10.4089
10.1128/MCB.10.10.5226
10.1101/gad.4.4.503
10.1002/j.1460-2075.1992.tb05568.x
{'key': 'e_1_2_3_31_2', 'first-page': '887', 'article-title': 'A yeast ARS‐binding protein activates transcription synergistically in combination with other weak activating factors', 'volume': '10', 'author': 'Buchrnan A. R.', 'year': '1990', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / A yeast ARS‐binding protein activates transcription synergistically in combination with other weak activating factors by Buchrnan A. R. (1990)
10.1128/MCB.10.5.2437 / Mol. Cell. Biol. / The ABF1 factor is the transcriptional activator of the L2 ribosomal protein genes in Saccharomyces cerevisiae by Della Seta F. (1990)
10.1128/MCB.12.9.4197
10.1002/j.1460-2075.1987.tb04776.x
10.1128/MCB.10.9.4872
10.1093/nar/18.18.5393
10.1128/MCB.13.1.543
10.1128/MCB.13.4.2623
{'key': 'e_1_2_3_39_2', 'volume-title': 'Molecular cloning: a laboratory manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular cloning: a laboratory manual by Sambrook J. (1989)
10.1002/yea.320020304
10.1016/0076-6879(87)54085-X
10.1128/MCB.6.10.3320
10.1073/pnas.89.21.10232
10.1073/pnas.73.2.386
10.1007/BF00447039
10.1016/S0021-9258(18)77354-7 / J. Biol. Chem. / Contacts between the factor TUF and RPG sequences by Vignais M.‐L. (1990)
10.1093/nar/22.2.124
{'key': 'e_1_2_3_48_2', 'first-page': '276', 'article-title': 'Identification of the upstream activation sequences responsible for the expression and regulation of the PEM1 and PEA2 genes encoding the enzymes of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae', 'volume': '109', 'author': 'Kodaki T.', 'year': '1991', 'journal-title': 'J. Biochem.'} / J. Biochem. / Identification of the upstream activation sequences responsible for the expression and regulation of the PEM1 and PEA2 genes encoding the enzymes of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae by Kodaki T. (1991)
10.1093/nar/22.3.412
10.1128/MCB.10.2.859
10.1093/nar/17.13.4917
10.1128/MCB.12.12.5516
10.1016/0092-8674(89)90788-5
10.1016/S0021-9258(18)82077-4

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 6:01 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:50 p.m.)
Indexed	3 months, 2 weeks ago (May 16, 2025, 3:27 a.m.)
Issued	30 years, 11 months ago (Oct. 1, 1994)
Published	30 years, 11 months ago (Oct. 1, 1994)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	30 years, 11 months ago (Oct. 1, 1994)

Funders 0

None

BibTeX

@article{Sch_ller_1994, title={Importance of General Regulatory Factors Rap1p, Abf1p and Reb1p for the Activation of Yeast Fatty Acid Synthase Genes FAS1 and FAS2}, volume={225}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1994.00213.x}, DOI={10.1111/j.1432-1033.1994.00213.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Schüller, Hans‐Joachim and Schütz, Armin and Knab, Sabine and Hoffmann, Brigitte and Schweizer, Eckhart}, year={1994}, month=oct, pages={213–222} }

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