DOI: 10.1111/j.1432-1033.1993.tb19908.x. Chemical assay for cyst(e)ine‐rich peptides detects a novel intestinal peptide ZF‐1, homologous to a single zinc‐finger motif

Chemical assay for cyst(e)ine‐rich peptides detects a novel intestinal peptide ZF‐1, homologous to a single zinc‐finger motif

10.1111/j.1432-1033.1993.tb19908.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Cysteine is a relatively infrequent constituent of proteins, which in its thiol or half‐cystine form contributes in a special manner to their three‐dimensional structure. We show that in small cystine‐containing peptides, the Cys content is always higher than the average in proteins in general. This observation makes it possible to search for new peptides by monitoring only their Cys content. We have developed a chemical assay for the detection of cyst(e)ine‐rich peptides in tissue extracts. Using this assay we have isolated from porcine intestine a novel cysteine‐rich peptide, which we denote ZF‐1. ZF‐1 is homologous to a single zinc‐finger motif and has an acetylated N‐terminus. This is the first demonstration of the existence of a processed single zinc‐finger‐like structure. The structural homology of ZF‐1 to the zinc‐finger motif, present in several metal‐binding and DNA‐binding proteins, suggests an important role of this peptide in metal transport and/or modulation of gene expression.

Bibliography

SILLARD, R., JÃRNVALL, H., CARLQUIST, M., & MUTT, V. (1993). Chemical assay for cyst(e)ineârich peptides detects a novel intestinal peptide ZFâ1, homologous to a single zincâfinger motif. European Journal of Biochemistry, 211(1â2), 377â380. Portico.

Authors 4

Rannar SILLARD (first)
Hans JÖRNVALL (additional)
Mats CARLQUIST (additional)
Viktor MUTT (additional)

References 32 Referenced 12

10.1007/978-3-642-71534-1_20
{'key': 'e_1_2_3_3_2', 'first-page': '21', 'volume-title': 'Gut hormones', 'author': 'Mutt V.', 'year': '1978'} / Gut hormones by Mutt V. (1978)
10.1111/j.1432-1033.1972.tb01903.x
{'key': 'e_1_2_3_5_2', 'first-page': '30', 'volume-title': 'Laboratory techniques in biochemistry and molecular biology', 'author': 'Allen G.', 'year': '1981'} / Laboratory techniques in biochemistry and molecular biology by Allen G. (1981)
Sillard R. Rökaeus Å. Xu Y. Carlquist M. Bergman T. Jörnvall H.&Mutt V.(1992)Peptides13 in the press. (10.1016/0196-9781(92)90005-N)
10.1016/0006-291X(77)90523-X
10.1093/nar/18.13.3871
10.1073/pnas.83.8.2516
10.1016/0303-7207(89)90010-5
10.1128/MCB.9.5.2124
10.1038/344876a0
10.1016/0042-6822(87)90399-0
10.1038/344879a0
10.1016/0092-8674(88)90174-2
10.1073/pnas.88.21.9671
10.1146/annurev.bi.61.070192.005201
10.1126/science.3047872
10.1016/S0021-9258(19)50405-7
10.1007/978-1-349-12664-4_1 / Galanin: a new multifunctional peptide in the neuro‐endocrine system by Mutt V. (1991)
10.1073/pnas.84.23.8628
10.1073/pnas.59.4.1321
10.1016/0006-291X(84)91077-5
10.1073/pnas.86.21.8590
10.1126/science.179.4068.77
10.1172/JCI112121
10.1038/332411a0
10.1016/S0021-9258(18)49238-1
10.1016/0006-3002(53)90173-5
10.1073/pnas.89.3.947
10.1073/pnas.73.10.3413
10.1073/pnas.77.7.4011
10.1016/0014-5793(83)80469-4

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 2:58 a.m.)
Deposited	2 years ago (Aug. 31, 2023, 12:21 a.m.)
Indexed	1 year, 5 months ago (March 28, 2024, 3:17 p.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{SILLARD_1993, title={Chemical assay for cyst(e)ine‐rich peptides detects a novel intestinal peptide ZF‐1, homologous to a single zinc‐finger motif}, volume={211}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1993.tb19908.x}, DOI={10.1111/j.1432-1033.1993.tb19908.x}, number={1–2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SILLARD, Rannar and JÖRNVALL, Hans and CARLQUIST, Mats and MUTT, Viktor}, year={1993}, month=jan, pages={377–380} }

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