DOI: 10.1111/j.1432-1033.1993.tb18452.x. Secretory leucocyte proteinase inhibitor is produced by human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes

Secretory leucocyte proteinase inhibitor is produced by human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes

10.1111/j.1432-1033.1993.tb18452.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The objective of this study was to examine the expression of the secretory leucocyte proteinase inhibitor (SLPI) gene by human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes. RNA was extracted from human articular cartilage chondrocytes, synovial fibroblasts and fibrochondrocytes of the annulus fibrosus. Expression was analyzed by Northern blotting and hybridisation to a SLPI RNA probe. SLPI was isolated from chondrocyte culture supernatant by gelpermeation and cation‐exchange chromatography and examined by a solid‐phase enzyme‐linked immunofiltration assay for SLPI and by SDS/PAGE using Western blotting with polyclonal IgG samples against bronchial mucus leucocyte proteinase inhibitor and SLPI. This identified biochemically and immunologically the major serine proteinase inhibitory protein synthesised by chondrocytes as SLPI. Secretory leucoyte proteinase inhibitor mRNA was detected in RNA preparations from articular cartilage chondrocytes and annulus fibrosus fibrochondrocytes. The results presented here allowed us to deduce that human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes synthesized SLPI whereas human synovial fibroblasts did not.

Bibliography

JACOBY, A. S., MELROSE, J., ROBINSON, B. G., HYLAND, V. J., & GHOSH, P. (1993). Secretory leucocyte proteinase inhibitor is produced by human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes. European Journal of Biochemistry, 218(3), 951â957. Portico.

Authors 5

Arie S. JACOBY (first)
James MELROSE (additional)
Bruce G. ROBINSON (additional)
Valentine J. HYLAND (additional)
Peter GHOSH (additional)

References 23 Referenced 18

10.1172/JCI115255
10.1002/art.1780330911
10.1515/bchm3.1992.373.1.111
10.1042/bj2740269
10.1111/j.1432-1033.1992.tb17108.x / Eur. J. Biochem. / The serine protease inhibitor of cartilage matrix is not a chondrocytic gene product by Böm B. (1992)
10.1016/0003-2697(87)90021-2
10.1016/0049-0172(91)90023-S
{'key': 'e_1_2_3_9_1', 'first-page': '79', 'article-title': 'Human mucus proteinase inhibitor (human MPI)', 'volume': '369', 'author': 'Fritz H.', 'year': '1988', 'journal-title': 'Biol. Chem. Hoppe Seyler'} / Biol. Chem. Hoppe Seyler / Human mucus proteinase inhibitor (human MPI) by Fritz H. (1988)
{'key': 'e_1_2_3_10_1', 'first-page': '198', 'article-title': 'The latent serine proteinases of normal and degenerate (OA) human cartilage', 'volume': '16', 'author': 'Ghosh P.', 'year': '1991', 'journal-title': 'Trans. Orthop. Res. Soc.'} / Trans. Orthop. Res. Soc. / The latent serine proteinases of normal and degenerate (OA) human cartilage by Ghosh P. (1991)
10.1016/0304-4165(79)90276-9
10.1111/j.1462-5822.2007.00901.x
10.1016/0009-8981(87)90383-4
{'key': 'e_1_2_3_14_1', 'first-page': '448', 'volume-title': 'Molecular cloning: a laboratory manual', 'author': 'Maniatis T.', 'year': '1982'} / Molecular cloning: a laboratory manual by Maniatis T. (1982)
10.1016/S0934-8832(11)80090-9
{'key': 'e_1_2_3_16_1', 'article-title': 'A solid phase enzyme linked immunosorbent assay for secretory leucocyte proteinase inhibitor', 'author': 'Melrose J.', 'year': '1993', 'journal-title': 'Clin. Chim. Acta'} / Clin. Chim. Acta / A solid phase enzyme linked immunosorbent assay for secretory leucocyte proteinase inhibitor by Melrose J. (1993)
10.1016/0003-2697(87)90587-2
10.1046/j.1365-2222.1998.00312.x
10.1093/oxfordjournals.jbchem.a132305
10.1093/nar/14.20.7883
10.1073/pnas.76.9.4350
10.3233/BIR-1978-153-409
10.1016/0049-0172(81)90048-2 / Semin. Arthritis Rheum. / The role of serine protease inhibitors in normal and osteoarthritic human articular cartilage by Walton E. A. (1981)
{'key': 'e_1_2_3_24_1', 'first-page': '37', 'volume-title': 'Matrix metalloproteinases and inhibitors', 'author': 'Wilhelm S. M.', 'year': '1992'} / Matrix metalloproteinases and inhibitors by Wilhelm S. M. (1992)

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 4:33 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:11 p.m.)
Indexed	1 year, 2 months ago (June 15, 2024, 1:57 p.m.)
Issued	31 years, 9 months ago (Dec. 1, 1993)
Published	31 years, 9 months ago (Dec. 1, 1993)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	31 years, 9 months ago (Dec. 1, 1993)

Funders 0

None

BibTeX

@article{JACOBY_1993, title={Secretory leucocyte proteinase inhibitor is produced by human articular cartilage chondrocytes and intervertebral disc fibrochondrocytes}, volume={218}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1993.tb18452.x}, DOI={10.1111/j.1432-1033.1993.tb18452.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={JACOBY, Arie S. and MELROSE, James and ROBINSON, Bruce G. and HYLAND, Valentine J. and GHOSH, Peter}, year={1993}, month=dec, pages={951–957} }

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