DOI: 10.1111/j.1432-1033.1993.tb18117.x. An arrestin homolog of blowfly photoreceptors stimulates visual‐pigment phosphorylation by activating a membrane‐associated protein kinase

An arrestin homolog of blowfly photoreceptors stimulates visual‐pigment phosphorylation by activating a membrane‐associated protein kinase

10.1111/j.1432-1033.1993.tb18117.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

An arrestin homolog (Arr2, 49‐kDa protein) of blowfly (Calliphora erythrocephala) retinae undergoes light‐dependent reversible binding to the photoreceptor membrane. In order to characterize this arrestin homolog and to study its function in a well‐defined experimental system, we developed a purification scheme which used microvillar photoreceptor membranes as an affinity binding matrix. Additional purification steps included ammonium sulfate precipitation, gel filtration and binding to heparin‐agarose. The molecular mass of purified Arr2, as judged by SDS/PAGE, is in the range 45–49 kDa. The isoelectric point, as judged by gel isolelectric focussing, is 8.7. Arr2 is specific to the retina, where it is subject to phosphorylation at multiple sites.Binding of purified Arr2 to isolated photoreceptor membranes efficiently activates the light‐induced phosphorylation of visual pigment. Since the assay system used is deficient in rhodopsin phosphatase activity, the arrestin‐stimulated phosphate incorporation into rhodopsin results solely from the activation of a protein kinase. Phosphorylation experiments with highly purified membrane preparations indicate that rhodopsin kinase is tightly associated with the rhabdomeric membrane or the microvillar cytoskeleton. Rhodopsin kinase is released from the membrane or inactivated upon treatment with urea. It is concluded that this arrestin is a regulator protein that controls visual‐pigment phosphorylation by affecting the interaction of metarhodopsin and rhodopsin (metarhodopsin) kinase.

Bibliography

BENTROP, J., PLANGGER, A., & PAULSEN, R. (1993). An arrestin homolog of blowfly photoreceptors stimulates visualâpigment phosphorylation by activating a membraneâassociated protein kinase. European Journal of Biochemistry, 216(1), 67â73. Portico.

Authors 3

Joachim BENTROP (first)
Anette PLANGGER (additional)
Reinhard PAULSEN (additional)

References 33 Referenced 30

10.1111/j.1432-1033.1986.tb10124.x
10.1073/pnas.90.5.1907
10.1021/ja01482a024
10.1111/j.1432-1033.1992.tb17379.x
10.1016/S0021-9258(19)37139-X / J. Biol. Chem. / Activation of bovine rod outer segment phospholipase C by arrestin by Ghalayini A. J. (1992)
10.1007/978-3-642-85769-0_18
10.1007/BF00535444
10.1002/elps.1150090106
10.1073/pnas.87.3.1008
10.1038/227680a0
10.1016/0925-4773(90)90131-5
10.1126/science.6419348
10.1016/S0021-9258(19)41320-3 / J. Biol. Chem / Phosphorylation and dephosphorylation of frog rod outer segment membranes as part of the visual process by Miller J. A. (1975)
{'key': 'e_1_2_3_15_1', 'first-page': '99', 'volume-title': 'Progress in retinal research', 'author': 'Minke B.', 'year': '1991'} / Progress in retinal research by Minke B. (1991)
{'key': 'e_1_2_3_16_1', 'first-page': '502', 'article-title': 'A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels', 'volume': '56', 'author': 'Oakley B. R.', 'year': '1980', 'journal-title': 'Anal. Biochem.'} / Anal. Biochem. / A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels by Oakley B. R. (1980)
{'key': 'e_1_2_3_17_1', 'first-page': '1', 'volume-title': 'Molecular biology of the retina: basic and clinically relevant studies', 'author': 'Pak W. L.', 'year': '1991'} / Molecular biology of the retina: basic and clinically relevant studies by Pak W. L. (1991)
10.1016/S0021-9258(18)71543-3 / J. Biol. Chem. / Regulation of rhodopsin dephosphorylation by arrestin by Palczewski K. (1989)
10.1016/S0021-9258(18)98620-5 / J. Biol. Chem. / Role of the carboxyl‐terminal region of arrestin in binding to phosphorylated rhodopsin by Palczewski K. (1991)
10.1016/S0021-9258(18)98787-9
10.1111/j.1432-1033.1983.tb07869.x
10.1007/BF00610930
10.1007/BF00610929
{'key': 'e_1_2_3_24_1', 'first-page': '227', 'volume-title': 'Proceedings of the yamada conference', 'author': 'Paulsen R.', 'year': '1988'} / Proceedings of the yamada conference by Paulsen R. (1988)
10.1042/bj0350461
10.1083/jcb.116.3.683 / J. Cell Biol. / Differential localizations of and requirements for the two Drosophila ninaC kinase/myosins in photoreceptor cells by Porter J. A. (1992)
10.1038/356336a0 / Nature / Rhodopsin inactivation is a modulated process in Limulus photoreceptors by Richard E. A. (1992)
10.1021/bi00430a052
10.1073/pnas.87.3.1003
10.1146/annurev.cb.07.110191.001113
10.1016/S0091-679X(08)60020-5
10.1073/pnas.83.5.1174
10.1126/science.2158671
10.1016/0014-5793(86)80008-4 / FEBS Lett. / A 48 kDa protein arrests cGMP phosphodiesterase activation in retinal rod disk membranes by Zukerman R. (1986)

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 3:50 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 10:16 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 7, 2024, 4:41 p.m.)
Issued	32 years ago (Aug. 1, 1993)
Published	32 years ago (Aug. 1, 1993)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	32 years ago (Aug. 1, 1993)

Funders 0

None

BibTeX

@article{BENTROP_1993, title={An arrestin homolog of blowfly photoreceptors stimulates visual‐pigment phosphorylation by activating a membrane‐associated protein kinase}, volume={216}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1993.tb18117.x}, DOI={10.1111/j.1432-1033.1993.tb18117.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BENTROP, Joachim and PLANGGER, Anette and PAULSEN, Reinhard}, year={1993}, month=aug, pages={67–73} }

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