DOI: 10.1111/j.1432-1033.1992.tb19827.x. Effect of dexamethasone on mRNA levels for 5‐aminolevulinate synthase in different rat tissues

Effect of dexamethasone on mRNA levels for 5‐aminolevulinate synthase in different rat tissues

10.1111/j.1432-1033.1992.tb19827.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

5‐Aminolevulinate synthase mRNA levels from different tissues were quantitated by Northern blot hybridization analysis utilizing the rat liver 5‐aminolevulinate synthase cDNA clone as probe. A 5‐aminolevulinate synthase mRNA species of size 2.3 kb was seen in all the tissues examined. Densitometric scanning of the autoradiographs demonstrated that the adrenal gland contained the largest amount of 5‐aminolevulinate synthase mRNA. Levels corresponding to approximately 50% of this amount were found in the small intestine, lung, heart, muscle and testes. In the liver and kidney the level was approximately 25% of that found in the adrenal gland. These results demonstrate the housekeeping role of this gene. Dexamethasone treatment for 1 day or 5 days dramatically induced 5‐aminolevulinate synthase mRNA levels in the liver and small intestine, and to a lesser extent in lung, heart, kidney and muscle. Nuclear run‐off experiments suggest that a post‐transcriptional mechanism predominantly contributes to the dexamethasone‐induced increase in 5‐aminolevulinate synthase mRNA levels observed in the liver. Interestingly, in the steroidogenic tissues of the adrenal gland and testes, there was a substantial decrease in 5‐aminolevulinate synthase mRNA levels after dexamethasone administration but the mechanism of this control remains to be investigated.

Bibliography

SRIVASTAVA, G., KWONG, S. K. Y., LAM, K. S. L., & MAY, B. K. (1992). Effect of dexamethasone on mRNA levels for 5âaminolevulinate synthase in different rat tissues. European Journal of Biochemistry, 203(1â2), 59â63. Portico.

Authors 4

Gopesh SRIVASTAVA (first)
Simon K. Y. KWONG (additional)
Karen S. L. LAM (additional)
Brian K. MAY (additional)

References 18 Referenced 7

{'key': 'e_1_2_2_2_2', 'first-page': '282', 'volume': '25', 'author': 'Bottomley S. S.', 'year': '1988', 'journal-title': 'Semin. Hematol.'} / Semin. Hematol. by Bottomley S. S. (1988)
{'key': 'e_1_2_2_3_2', 'first-page': '1305', 'volume-title': 'The metabolic basis of inherited disease', 'author': 'Kappas A.', 'year': '1989'} / The metabolic basis of inherited disease by Kappas A. (1989)
{'key': 'e_1_2_2_4_2', 'first-page': '150', 'volume': '23', 'author': 'May B. K.', 'year': '1989', 'journal-title': 'Semir. Hematol.'} / Semir. Hematol. by May B. K. (1989)
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Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 12:59 a.m.)
Deposited	2 years ago (Sept. 1, 2023, 5:43 p.m.)
Indexed	1 year, 10 months ago (Oct. 12, 2023, 6:15 p.m.)
Issued	33 years, 8 months ago (Jan. 1, 1992)
Published	33 years, 8 months ago (Jan. 1, 1992)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	33 years, 8 months ago (Jan. 1, 1992)

Funders 0

None

BibTeX

@article{SRIVASTAVA_1992, title={Effect of dexamethasone on mRNA levels for 5‐aminolevulinate synthase in different rat tissues}, volume={203}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb19827.x}, DOI={10.1111/j.1432-1033.1992.tb19827.x}, number={1–2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SRIVASTAVA, Gopesh and KWONG, Simon K. Y. and LAM, Karen S. L. and MAY, Brian K.}, year={1992}, month=jan, pages={59–63} }

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