DOI: 10.1111/j.1432-1033.1992.tb17368.x. Characterization of the 56‐kDa subunit of yeast trehalose‐6‐phosphate synthase and cloning of its gene reveal its identity with the product of <i>CIF1</i>, a regulator of carbon catabolite inactivation

Characterization of the 56‐kDa subunit of yeast trehalose‐6‐phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation

10.1111/j.1432-1033.1992.tb17368.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Trehalose‐6‐phosphate synthase is the key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells of yeast. This enzyme was purified from a strain of Saccharomyces cerevisiae lacking vacuolar proteases. It was found to be a multimeric protein of 630 kDa. Monoclonal antibodies were raised against its smallest subunit (56 kDa) and used for screening a yeast cDNA library. This yielded an immunopositive cDNA clone of 1.7 kb, containing an open reading frame of 1485 base pairs. Its sequence, called TPS1 (for trehalose‐6‐phosphate synthase), was represented by a single gene in the yeast genome and was found to be almost identical with the recently sequenced CIF1, a gene important for carbon catabolite inactivation, believed to be allelic with FDP1. A mutant obtained by disruption of TPS1 had a very low activity of trehalose‐6‐phosphate synthase, indicating that TPS1 is an important component of the enzyme. The mutant also showed a growth defect when transferred from glycerol to glucose, a phenotype similar to that of the cif1 and fdp1 mutants deficient in carbon catabolite inactivation. Thus, the smallest subunit of the biosynthetic enzyme trehalose‐6‐phosphate synthase appears to have, in addition, a central regulatory role in the carbohydrate metabolism of yeast.

Bibliography

BELL, W., KLAASSEN, P., OHNACKER, M., BOLLER, T., HERWEIJER, M., SCHOPPINK, P., VANDERZEE, P., & WIEMKEN, A. (1992). Characterization of the 56âkDa subunit of yeast trehaloseâ6âphosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation. European Journal of Biochemistry, 209(3), 951â959. Portico.

Authors 8

Walter BELL (first)
Paul KLAASSEN (additional)
Martin OHNACKER (additional)
Thomas BOLLER (additional)
Marga HERWEIJER (additional)
Peter SCHOPPINK (additional)
Peter VANDERZEE (additional)
Andres WIEMKEN (additional)

References 43 Referenced 199

{'key': 'e_1_2_3_2_1', 'first-page': '13334', 'article-title': 'Proteolysis in eucaryotic cells', 'volume': '259', 'author': 'Achstetter T.', 'year': '1984', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Proteolysis in eucaryotic cells by Achstetter T. (1984)
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10.1128/MCB.2.8.921
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10.1016/0003-2697(76)90527-3
10.1016/S0021-9258(19)77303-7 / J. Biol. Chem. / The biosynthesis of trehalose‐6‐phosphate by Cabib E. (1958)
{'key': 'e_1_2_3_9_1', 'first-page': '447', 'article-title': 'Investigation of the relationship between sst1 and fdp mutations in yeast and their effect on trehalose synthesis', 'volume': '18', 'author': 'Charlab R.', 'year': '1985', 'journal-title': 'Braz. J. Med. Biol. Res'} / Braz. J. Med. Biol. Res / Investigation of the relationship between sst1 and fdp mutations in yeast and their effect on trehalose synthesis by Charlab R. (1985)
10.1016/0014-5793(90)81062-S
10.1016/0014-5793(91)81008-V
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{'key': 'e_1_2_3_13_1', 'first-page': '17', 'article-title': 'Trehalose‐6‐Phosphat‐Synthase aus Bäckerhefe', 'volume': '31', 'author': 'Elander M.', 'year': '1968', 'journal-title': 'Arkiv för Kemi'} / Arkiv för Kemi / Trehalose‐6‐Phosphat‐Synthase aus Bäckerhefe by Elander M. (1968)
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10.1016/S0021-9258(18)94254-7 / J. Biol. Chem / A conserved gene encoding the 57‐kDa subunit of the yeast vacuolar H+ ATPase by Nelson H. (1989)
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10.1007/BF00384607
10.1111/j.1574-6968.1988.tb02724.x
{'key': 'e_1_2_3_30_1', 'volume-title': 'Molecular cloning: a laboratory manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular cloning: a laboratory manual by Sambrook J. (1989)
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{'key': 'e_1_2_3_33_1', 'volume-title': 'Methods in yeast genetics', 'author': 'Sherman F.', 'year': '1986'} / Methods in yeast genetics by Sherman F. (1986)
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10.1111/j.1574-6968.1989.tb03396.x / FEMS Microbiol. Rev. / Trehalose, reserve and/or stress metabolite by Van Laere A. (1989)
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10.1111/j.1574-6968.1989.tb03636.x
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10.1002/j.1460-2075.1987.tb02435.x

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 2:45 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 5:08 a.m.)
Indexed	22 hours, 15 minutes ago (Aug. 26, 2025, 2:44 a.m.)
Issued	32 years, 9 months ago (Nov. 1, 1992)
Published	32 years, 9 months ago (Nov. 1, 1992)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	32 years, 9 months ago (Nov. 1, 1992)

Funders 0

None

BibTeX

@article{BELL_1992, title={Characterization of the 56‐kDa subunit of yeast trehalose‐6‐phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation}, volume={209}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb17368.x}, DOI={10.1111/j.1432-1033.1992.tb17368.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BELL, Walter and KLAASSEN, Paul and OHNACKER, Martin and BOLLER, Thomas and HERWEIJER, Marga and SCHOPPINK, Peter and VANDERZEE, Peter and WIEMKEN, Andres}, year={1992}, month=nov, pages={951–959} }

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