DOI: 10.1111/j.1432-1033.1992.tb17325.x. X‐ray crystallographic characterisation of type‐2‐depleted ascorbate oxidase from zucchini

X‐ray crystallographic characterisation of type‐2‐depleted ascorbate oxidase from zucchini

10.1111/j.1432-1033.1992.tb17325.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The type‐2 depleted form of ascorbate oxidase from zucchini has been prepared in crystals and characterised by X‐ray crystallography and EPR spectroscopy. The X‐ray structure analysis by difference‐Fourier techniques and refinement shows that, on average, about 1.3 Cu atoms/ascorbate oxidase monomer are removed. The copper is lost from the trinuclear site whereby the EPR‐active type‐2 copper is depleted most; type‐1 copper is not affected. This observation indicates preferential formation of a 1 Cu‐depleted form with the hole equally distributed over all three copper sites. Each of these 1 Cu‐depleted species may represent an anti‐ferromagnetically coupled copper pair which is EPR‐silent and could explain the disappearance of the type‐2 EPR signal.

Bibliography

MESSERSCHMIDT, A., STEIGEMANN, W., HUBER, R., LANG, G., & KRONECK, P. M. H. (1992). Xâray crystallographic characterisation of typeâ2âdepleted ascorbate oxidase from zucchini. European Journal of Biochemistry, 209(2), 597â602. Portico.

Authors 5

Albrecht MESSERSCHMIDT (first)
Wolfgang STEIGEMANN (additional)
Robert HUBER (additional)
Gunter LANG (additional)
Peter M. H. KRONECK (additional)

References 40 Referenced 47

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Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 2:34 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:29 p.m.)
Indexed	1 week, 5 days ago (Aug. 24, 2025, 7:02 p.m.)
Issued	32 years, 11 months ago (Oct. 1, 1992)
Published	32 years, 11 months ago (Oct. 1, 1992)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	32 years, 11 months ago (Oct. 1, 1992)

Funders 0

None

BibTeX

@article{MESSERSCHMIDT_1992, title={X‐ray crystallographic characterisation of type‐2‐depleted ascorbate oxidase from zucchini}, volume={209}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb17325.x}, DOI={10.1111/j.1432-1033.1992.tb17325.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MESSERSCHMIDT, Albrecht and STEIGEMANN, Wolfgang and HUBER, Robert and LANG, Gunter and KRONECK, Peter M. H.}, year={1992}, month=oct, pages={597–602} }

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