DOI: 10.1111/j.1432-1033.1992.tb17237.x. Precursor of mitochondrial aspartate aminotransferase synthesized in <i>Escherichia coli</i> is complexed with heat‐shock protein DnaK

Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat‐shock protein DnaK

10.1111/j.1432-1033.1992.tb17237.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

On expression of the cDNA encoding the precursor of chicken mitochondrial aspartate aminotransferase (pmAspAT) in Escherichia coli, the bulk of pmAspAT was found to be associated with the 70‐kDa heat‐shock protein DnaK which is closely related to mitochondrial 70‐kDa heat‐shock protein (HSP70). Purification protocols for the DnaK/pmAspAT complex and its individual components were elaborated. The complex dissociated on treatment with MgATP or at pH 5.5. Like the mature enzyme, pmAspAT is a dimer (2 × 47 kDa) and exhibits about a third of its enzyme activity. In the DnaK/pmAspAT complex, one DnaK molecule is bound to each subunit of pmAspAT; this tetramer may further aggregate to an octamer. The complex is catalytically almost as active as free pmAspAT. It could be reconstituted from isolated DnaK and pmAspAT. No complex was formed with mAspAT. Apparently, DnaK binds to the solvent‐exposed presequence of folded pmAspAT without significantly changing the structure and functional properties of its mature moiety.

Bibliography

SCHMID, D., JAUSSI, R., & CHRISTEN, P. (1992). Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heatâshock protein DnaK. European Journal of Biochemistry, 208(3), 699â704. Portico.

Authors 3

Daniel SCHMID (first)
Rolf JAUSSI (additional)
Philipp CHRISTEN (additional)

References 33 Referenced 29

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{'key': 'e_1_2_3_9_1', 'first-page': '97', 'volume': '115', 'author': 'Gehring H.', 'year': '1977', 'journal-title': 'J. Biol. Chem'} / J. Biol. Chem by Gehring H. (1977)
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10.1073/pnas.80.21.6431

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 2:16 a.m.)
Deposited	1 year, 11 months ago (Oct. 1, 2023, 4:41 a.m.)
Indexed	4 months ago (May 6, 2025, 11:52 a.m.)
Issued	33 years ago (Sept. 1, 1992)
Published	33 years ago (Sept. 1, 1992)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	33 years ago (Sept. 1, 1992)

Funders 0

None

BibTeX

@article{SCHMID_1992, title={Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat‐shock protein DnaK}, volume={208}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb17237.x}, DOI={10.1111/j.1432-1033.1992.tb17237.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SCHMID, Daniel and JAUSSI, Rolf and CHRISTEN, Philipp}, year={1992}, month=sep, pages={699–704} }

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