DOI: 10.1111/j.1432-1033.1992.tb17146.x. Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochrome‐c1

Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochrome‐c1– heme lyase

10.1111/j.1432-1033.1992.tb17146.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Cytochrome c1, a subunit of the mitochondrial ubiquinol–cytochrome‐c reductase, is synthesized on cytosolic ribosomes as a precusor protein of 37 kDa. Maturation to the mature 31‐kDa form involves two proteolytic processing steps of the amino‐terminal presequnce. After removal of the amino‐terminal part by the matrix‐localized processing peptidase, the carboxy‐terminal part of the presequence is cleaved off by an unknown intermembrane space protease. This step depends on covalent linkage of heme to the apoprotein. At least two complementation groups (I and II) can be distinguished among mutants of the yeast Saccharomyces cerevisiae, which are defective in this second proteolytic processing, i.e. they accumulate the intermediate‐sized form of cytochrome c1 instead of for cytochrome c1 [Sadler, I., Suda, K., Schatz, G., Kaudewitz, F. & Haid, A., (1984) EMBO J. 3, 2137–2143]. We report on the molecular cloning and characterization of the CYT2 gene representing complementation group I. It maps on chromosome XI and encodes a mitochondrial protein of about 26 kDa. Extensive similarity to Neurospora crassa and S. cerevisiae cytochrome‐c–heme lyase, as well as the phenotype of cyt2 mutants, strogly suggest that we have identified the gene for cytochrome‐c1–heme lyase.

Bibliography

ZOLLNER, A., RÃDEL, G., & HAID, A. (1992). Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochromeâc1â heme lyase. European Journal of Biochemistry, 207(3), 1093â1100. Portico.

Authors 3

Alfred ZOLLNER (first)
Gerhard RÖDEL (additional)
Albert HAID (additional)

References 41 Referenced 88

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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 2:16 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 5 a.m.)
Indexed	2 months ago (June 16, 2025, 2:05 p.m.)
Issued	33 years ago (Aug. 1, 1992)
Published	33 years ago (Aug. 1, 1992)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	33 years ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{ZOLLNER_1992, title={Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochrome‐c1– heme lyase}, volume={207}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb17146.x}, DOI={10.1111/j.1432-1033.1992.tb17146.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={ZOLLNER, Alfred and RÖDEL, Gerhard and HAID, Albert}, year={1992}, month=aug, pages={1093–1100} }

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